Studies on the Preseut state of Laboratory Animal Allergy in Japan and its Preventive measures

日本实验动物过敏现状及预防措施研究

基本信息

批准号：
60480486
负责人：
CHUHEI Yamauchi
金额：
$ 4.35万
依托单位：
Kagoshima,University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1985
资助国家：
日本
起止时间：
1985 至 1987
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-60480486/
关键词：
Laboratory animal Allergy Allergen Animal room Dust particle 空調

项目摘要

Among 5,641 Persons in laboratory animal facilities of 137 research institutes, 1,304(23%) had one or more allergic symptoms associated with laboratory animals(LAA). Of allected individuals, 78% had nasal, 46% skin, 43% eye, 34% pulmonary symptoms. High incidence of LAA was found in the group of subjects who had daily contact with laboratory animals. The subjects of 70% developed LAA symptoms within three years of exposure. The individial which had the family history of allergy and with a history of previous allergy tended to developed LAA.The positive rate of some sensitive subjects to guinea-pigs by skin test using extracted substances were 100% for urine, 83% for epithelium and saliva and 67% for serum prepared from guinea-pigs. Urine antigen was protein of low molecular weight, and a part of this antigen was common to that of epithelium, saliva, and serum. The levels of total igE and RAST in the subjects tended to high than that of non-sensitive control group.In the animal room equiped with conventional turbulent flow air conditioning system, most of the protein was contained in dust particules over 1 <mu>m in size. For keeping air cleanliness in the working area of the animal room, we have developed a one-way flow air conditioning system in which the air flows the working area of the animal room through the rack and cages to exhaust side. The particle level in the working area of an animal room of turbulent flow system reached about class 10,000, however, the level of added sliding doors or curtains in front of the rack in the one-way-flow room was maintainedat class 100 with almost no dust particles over 1<mu> in size, except cage changing. Therefore, the wokers should be to wear a facemask when they work in animal room.

在137个研究所的实验动物设施中的5,641名人员中，1,304人（23%）患有一种或多种与实验动物（LAA）相关的过敏症状。在所有受影响的人中，78% 有鼻部症状，46% 有皮肤症状，43% 有眼部症状，34% 有肺部症状。日常接触实验动物的受试者中左心耳发生率较高。 70% 的受试者在接触后三年内出现左心耳症状。有过敏家族史和既往有过敏史的个体容易发生LAA。部分敏感对象对豚鼠进行提取物皮试，尿液阳性率为100%，上皮和唾液阳性率为83%豚鼠血清为 67%。尿抗原是低分子量蛋白质，该抗原的一部分与上皮、唾液和血清的抗原相同。受试者的总igE和RAST水平均高于非敏感对照组。在配备传统紊流空调系统的动物房中，大部分蛋白质包含在1μm以上的尘埃颗粒中。在尺寸上。为了保持动物房工作区域的空气清洁，我们开发了一种单向流空调系统，空气从动物房的工作区域流经架子和笼子到排气侧。紊流系统动物房工作区域的颗粒物等级达到10000级左右，而单向流房的架子前面加装推拉门或窗帘后，颗粒物等级基本维持在100级，几乎没有。尺寸超过 1μ> 的灰尘颗粒，更换笼子除外。因此，工作人员在动物房工作时应佩戴口罩。