Apoptosis in the process of carcinogenesis of oral precancerous lesions

口腔癌前病变癌变过程中的细胞凋亡

基本信息

批准号：
06454572
负责人：
NAGUMO Masao
金额：
$ 4.1万
依托单位：
Showa University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06454572/
关键词：
leukoplakia carcinogenesis apoptosis Le^y antigen P53 protein TGF-alpha epithelial dysplasia oncogene Le^y抗原 Tunel法抗癌剤 SNP 遺伝子異形成 P53 bcl-2

项目摘要

We investigated the expression of lewis^y (le^y) antigen, TGF-alpha, P53 protein, bcl-2 protein and PCNA immunohistochemically in the specimens which were obtained from patients with leukoplakia, cancer developed from leukoplakia and oral squamous cell carcinoma. Further, occurrence of apoptosis in those specimens was studied by detecting the fragmentation of DNA using Tunel method. In addition, we examined in vitro whether apoptosis could be induced in oral epithelial cells by anticancer drugs and sodium nitroprusside (SNP). We also examined the changes in the expression of genes during apoptosis.The results obtained were as follows :1.Le^y antigen and bcl-2 protein in oral cancer were expressed more weakly than those in leukoplakia. On the contrary, TGF-alpha, P53 protein and PCNA in oral cancer were expressed more strongly than those in leukoplakia.2.The expression of Le^y antigen and bcl-2 protein decreased with the grade of epithelial dysplasia in leukoplakia, whereas the expression of TGF-alpha, P53 protein and PCNA increased with the grade of epithelial dysplasia.3.The expression of Le^y antigen and bcl-2 protein decreased with malignant transformation.4.DNA fragmentation detected by Tunel method occurred more frequently in leukoplakia than in oral cancer.5-Fu, cisplatin and SNP could induced apoptosis in oral epithelial cells (NA) and mRNA expression of c-myc, c-myb, p53 was downregulated during apoptosis.

我们研究了Lewis^Y（Le^Y）抗原，TGF-Alpha，p53蛋白，Bcl-2蛋白和PCNA免疫组织化学中从白细胞中获得的标本中的癌症，从白细胞和口服鳞状细胞癌从白细胞中获得的癌症。此外，通过使用TUNEL方法检测DNA的碎片研究了这些标本中凋亡的发生。此外，我们在体外检查了是否可以通过抗癌药物和亚硝普钠（SNP）在口服上皮细胞中诱导凋亡。我们还检查了凋亡过程中基因表达的变化。所获得的结果如下：1.LE^Y抗原和Bcl-2蛋白在口腔癌中的表达比白细胞癌更弱。相反，口腔癌中的TGF-Alpha，p53蛋白和PCNA的表达比白细胞中的表达更强。2。le^y抗原和Bcl-2蛋白的表达随着白细胞增生的等级的降低，而在白细胞中的上皮性肿瘤等级，而TGF-Alpha和PCNA的表达却增加 dysplasia.3.The expression of Le^y antigen and bcl-2 protein decreased with malignant transformation.4.DNA fragmentation detected by Tunel method occurred more frequently in leukoplakia than in oral cancer.5-Fu, cisplatin and SNP could induced apoptosis in oral epithelial cells (NA) and mRNA expression of c-myc, c-myb, p53 was downregulated during apoptosis.