Fundermental study on clinical application of BMP for endodontics

BMP在牙髓治疗中的临床应用基础研究

• 批准号: 06454515
• 负责人: KAWAKAMI Toshiyuki
• 金额: $ 3.58万
• 依托单位: Matsumoto Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06454515/
• 关键词: BMP; evaluation; Image analysis; histological findings; squalane; osteoinduction; carrier; Mouse; マウス

BMP is an important bioactive protein whose bioactivity is facilitated by the delivery system. In clinical application of endodontics, it is necessary to use fluid carrier for BMP.Squalane is easily emulsified and has the property of spreading well. Thus, squalane might be effective in a BMP delivery system. Squalane/BMP (bovine derived partially purified) composite were implanted under the perimuscular membrane of ddY mice. For control, BMP only were used. The implants were roentgenographically and histologically examined at 1 to 4 weeks. According to the roentgenographical analysis, squalane/BMP composite specimens showed widespread heterotopic bone formation, and the amount was about 40% greater than that in the controls by a statistically analysis. Histological examination revealed varying amounts of new bone formed by perichondral ossification by 2 weeks. Observation of the bone of 3- and 4-week specimens indicated that bone marrow had already formed. These data suggest that squalane is effective for the slow local release of BMP.

BMP是一种重要的生物活性蛋白，其生物活性由递送系统促进。在牙齿牙髓病的临床应用中，有必要将流体载体用于BMP。平方很容易被乳化，并且具有良好的特性。因此，Squalane可能在BMP递送系统中有效。在DDY小鼠的周围膜下植入了孢子/BMP（牛衍生的部分纯化的牛）复合材料。为了控制，仅使用BMP。植入物在1到4周时在摩恩晶和组织学上进行了检查。根据roentgenographical分析，Squalane/BMP复合标本显示出广泛的异位骨形成，并且通过统计分析的对照组中的量比对照组的量大约40％。组织学检查表明，围绕蛋白座的骨化形成2周，形成的新骨变化了。对3周和4周标本的骨骼观察表明，骨髓已经形成。这些数据表明，Smocalane对BMP的局部释放有效。

项目成果

Kawakami,T.et al.: "A histopathological evaluation of the safety of squalane fluid applied subcutaneously." Med.Sci.Res.23. 521-523 (1995)

Kawakami,T.et al.：“皮下使用角鲨烷液安全性的组织病理学评估。”

武井則之,他: "スクアラン・BMP混合物の骨形成能の評価" 歯学基礎誌. 37補冊. 200 (1995)

Noriyuki Takei 等人：“角鲨烷/BMP 混合物的成骨潜力的评估”Basic Journal of Dentistry 37 增刊 200 (1995)。

Kawakami, T.et al.: "Evaluation of heterotopic bone formation induced by squalane/bone morphogenetic protein composite." Clinical Orthopaedics and Related Research. (in Press).

Kawakami, T.等人：“角鲨烷/骨形态发生蛋白复合物诱导的异位骨形成的评估。”

川上敏行: "スクアラン・BMP混合物による骨形成過程の病理組織学的観察" 歯科基礎医学会雑誌. 36. 203-203 (1994)

Toshiyuki Kawakami：“角鲨烷/BMP混合物对骨形成过程的组织病理学观察”日本基础牙科医学会杂志36. 203-203（1994）。

Kawakami, T. et al.: "A histopathological evaluation of the safety of squalane fluid applied subcutaneously." Med. Sci. Res.23:. 521-523, (1995)

Kawakami, T. 等人：“皮下使用角鲨烷液安全性的组织病理学评估。”