Morphological analysis of cardiac malformations in developing WKY/NCrj rat embryos raised in whole embryo

全胚培养WKY/NCrj大鼠胚胎心脏畸形的形态学分析

基本信息

批准号：
05670671
负责人：
NAKAGAWA Masao
金额：
$ 1.34万
依托单位：
Shiga University of Medical Science
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1994
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05670671/
关键词：
Whole embryo culture Sixth aortic arch HNK-1 N-CAM WKY NCrj rat Neural crest cell cardiac anomaly N-CAM

项目摘要

1.Morphological analysis of the heart in WKY/NCrj and control rat embryos raised in whole embryo culture.1) Sixth aortic arches were detected in both WKY/NCrj and control rat embryos cultured for 12 hours from 11.5 days of gestation. No morphological differences of the sixth aortic arches were observed between these two strains of embryos during 48 hours of culture.2) As the embryos cultured over 48 hours showed developmental delay compared with age-matched controls, this whole embryo culture system appeared useful only when performed for 48 hours.2.Immunolocalizations of extracellular molecules including migrating neural crest cell markers in the heart of WKY/NCrj and control rat embryos.HNK-1, N-CAM,fibronectine and cytotactin were immunopositive in the sixth aortic arches of both WKY/NCrj and control rat embryos at 11.5,12.5 and 13.5 day of gestation. The distributional patterns of those markers were almost same between WKY/NCrj and control rat embryos. N-CAM showed a chain of immunopositive tissues from nerve fibers around the foregut to the truncus arteriosus via the sixth aortic arch, which indicated that the sixth aortic arch was one of the pathways neural crest cells migrate through from splanchnic mesenchymal tissues to the truncus arteriosus. However, this study also suggested that cardiac malformations in WKY/NCrj rat might not be induced by hypoplastic sixth aortic arches or abnormal migrations of neural crest cells through this pathway.Conclusively, the hypoplastic sixth aortic arches in WKY/NCrj rat embryos at 14.5 day of gestation were supposed to be secondarily brought by an abnormal truncal division. Therefore, we are planning to clarify the distributional patterns or migratory pathways of cardiac neural crest cells which may play an important role in truncal division.

1.在WKY/NCRJ和整个胚胎培养中饲养的对照大鼠胚胎中心脏的术分析。1）在WKY/NCRJ和对照大鼠胚胎中都检测到第六个主动脉弓，从妊娠11.5天开始培养12小时。在培养的48小时内，这两种胚胎之间没有观察到第六个主动脉弓的形态学差异。2）2）由于与年龄匹配的对照相比，在48小时内培养的胚胎显示出发育迟缓，整个胚胎培养系统仅在48小时进行48小时时才能出现。对照大鼠的胚胎。HHNK-1，N-CAM，纤连蛋白和细胞肌动蛋白在WKY/NCRJ的第六个主动脉弓和对照大鼠胚胎的妊娠11.5,12.5和13.5天的第六个主动脉弓中是免疫阳性的。这些标记的分布模式在WKY/NCRJ和对照大鼠胚胎之间几乎相同。 N-CAM通过第六个主动脉弓从前肢周围的神经纤维到动脉的神经纤维的一系列免疫阳性组织链，这表明第六个主动脉弓是途径的神经crest细胞之一，从斑点间质质组织迁移到Truncus anteriosus。然而，这项研究还表明，WKY/NCRJ大鼠的心脏畸形可能不会由六型主动脉弓或神经crest细胞通过该途径的异常迁移引起，从而确定的是，在遗传下，WKY/NCRJ大鼠胚胎中的wky/ncrj大鼠胚胎中的六个主动脉弓可被遗传过。因此，我们计划阐明可能在截短分裂中起重要作用的心脏神经rest细胞的分布模式或迁移途径。