Role of Heat Shock Proteins in Physiology and Pathology of the Female Genital Tract

热激蛋白在女性生殖道生理学和病理学中的作用

• 批准号: 05454447
• 负责人: KONISHI Ikuo
• 金额: $ 4.42万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05454447/
• 关键词: Heat shock protein; HSP70; HSP90; Female genital tract; Endometrium; Endometrial cancer; Cervical cancer; Ovarian cancer

Ovarian sex steroids are involved in growth and differentiation of normal and neoplastic cells in the female genital tract. Although heat shock proteins HSP70 and HSP90 are known to modulate the intracellular interaction between the sex steroid and the respective receptor protein, little is known about expression and localization of HSPs in the human female genital tract. This study revealed that both HSP70 and HSP90 are expressed and specifically localized in the cells of normal endometrium, myometrium, fallopian tube, and cervix. Expression level of HSP70 changes in endometrium and myometrium during the menstrual cycle.Topological correlation between the HSPs and sex steroid receptors suggests that HSPs play an important role in the receptor-activation pathway in the endometrium. Endometrial hyperplasias/carcinomas and cervical carcinomas show an aberrant HSP70 expression. Ovarian cancers strongly express HSP70 along with p53 protein, which is inversely correlated with sex steroid receptor status. Consequently, both HSP70 and HSP90 are essential proteins in physiology and pathology of the female genital tract, and further studies are necessary to verify the exact function of these HSPs in the respective cells.

卵巢性类固醇参与女性生殖道正常细胞和肿瘤细胞的生长和分化。尽管已知热休克蛋白 HSP70 和 HSP90 可以调节性类固醇和相应受体蛋白之间的细胞内相互作用，但人们对 HSP 在人类女性生殖道中的表达和定位知之甚少。这项研究表明，HSP70 和 HSP90 均表达并特异性定位于正常子宫内膜、子宫肌层、输卵管和子宫颈的细胞中。子宫内膜和子宫肌层中HSP70的表达水平在月经周期中发生变化。HSPs与性类固醇受体之间的拓扑相关性表明HSPs在子宫内膜的受体激活途径中发挥着重要作用。子宫内膜增生/癌和宫颈癌显示异常的 HSP70 表达。卵巢癌强烈表达 HSP70 和 p53 蛋白，这与性类固醇受体状态呈负相关。因此，HSP70和HSP90都是女性生殖道生理和病理学中必需的蛋白质，需要进一步的研究来验证这些HSP在各自细胞中的确切功能。

项目成果

Koshiyama,M.,et al.: "Immunohistochemical analysis of p53 protein and 72kDa heat shock protein(HSP70)expression in ovarian carcinomas." Virchows Archiv. 427(in press). (1995)

Koshiyama, M., et al.：“卵巢癌中 p53 蛋白和 72kDa 热休克蛋白 (HSP70) 表达的免疫组织化学分析。”

Konishi, I., et al: "Sex steroid receptors, LH/hCG receptors, and oncogene expression in endometrial carcinomas" Gynecol Endocrinol. Vol.9 (in press). (1995)

Konishi, I. 等人：“子宫内膜癌中的性类固醇受体、LH/hCG 受体和癌基因表达”Gynecol Endocrinol。

Koshiyama,M.,et al.: "Immunohistochemical localization of heat shock proteins HSP70 and HSP90 in." Journal of Clinical Endocrinology and Metabolism. 80(in press). (1995)

Koshiyama, M., et al.：“热休克蛋白 HSP70 和 HSP90 的免疫组织化学定位。”

小西郁生: "子宮内膜の増殖・分化および癌化における性ステロイド受容体とストレス蛋白の発現" ホルモンと臨床. (掲載予定). (1994)

Ikuo Konishi：“性类固醇受体和应激蛋白在子宫内膜增殖、分化和癌变中的表达”《激素与临床科学》（待出版）。

小西 郁生: "子宮内膜の増殖・分化および癌化における性ステロイド受容体とストレス蛋白の発現" ホルモンと臨床. 42. 489-497 (1994)

Ikuo Konishi：“性类固醇受体和应激蛋白在子宫内膜增殖、分化和癌变中的表达”激素和临床研究 42. 489-497 (1994)。