Basic study of heart rate variability using chick embryo

利用鸡胚进行心率变异性的基础研究

• 批准号: 04807120
• 负责人: KOBAYASHI Kenji
• 金额: $ 1.28万
• 依托单位: Takushoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04807120/
• 关键词: heart beat; cardiotacogram; heart rate variability (HRV); PVDF-sensor; chick embryo; embryonic movement; FHR-deceleration; fetal heart rate (FHR); 胎児心拍数; PVDF

To make clear the relation of fetal heart rate variability (FHRV) with the environmental conditions, simulation experiments using chick embryo were made in following subjects.1) picking up heart beat noninvasively from chick embryo2) producing the cardiotacogram of chick embryo3) control temperature and humidity in experimental box4) measurement of HRV of chick embryo while changing temperature5) dependency of O_2 and CO_2 on HRV of chick embryo6) dependency of embryo movement on HRV of chick embryo7)influence of other environmental factors (intensive magnetic field, electro-magnetic wave, ultrasonic wave) to HRV of chick embryoThe subjects of 1), 2) and 3) which is bearing basic instrumentation were realized. Heart beat was detected by the two methods of optical plethysmograph and phonocardiograph from 4 days after incubation, and the heart rate variability was obtained in relation to change of the environmental factors such as temperature, O_2 and CO_2 concentration and embryonic movement. The results of the simulation experiments by chick embryo showed the analogous phenomena to fetal heart rate variability (FHRV) of human being.

为了清楚胎儿心率变异性（FHRV）与环境条件的关系，在以下受试者中进行了模拟实验。1）1）从雏鸡胚胎中无创的心脏跳动。实验盒中的湿度4）测量雏鸡胚胎的HRV同时变化温度5）O_2和CO_2对雏鸡胚胎的HRV的依赖性6）胚胎运动对雏鸡胚胎的HRV的依赖性对其他环境因素的影响（密集的磁场，电磁场，电磁场，电磁场） ，超声波）至1）和3）胚胎受试者的HRV实现了基本仪器。从孵育后的4天开始，通过光学量大分学和Phonocartiograph的两种方法检测到了心跳，并且与温度，O_2和CO_2浓度和胚胎运动等环境因素的变化有关，获得了心率变异性。雏鸡胚胎模拟实验的结果表明，与人类的胎儿心率变异性（FHRV）相似。

项目成果

野木 一広,長谷川 淳,小林 健二,近藤 俊吾: "発生期の心拍数変動の測定とその要因" 信学技報(MBE). 92-53. 15-20 (1992)

Kazuhiro Nogi、Jun Hasekawa、Kenji Kobayashi、Shungo Kondo：“发育过程中心率变异性的测量及其因素”IEICE 技术报告 (MBE) 92-53 (1992)。

Kobayashi, K.: "Heart Rate Measurement of Chick Embryo for Interpretation of Fetal Heart Rate Variability." Methods of Information in Medicine. vol.33 (Scheduled). (1994)

Kobayashi, K.：“鸡胚心率测量，用于解释胎儿心率变异性。”

Nogi, K.: "Measurements of the Embryonic Heart Rate Variability using Egg.(in Japanese)" Japanese Journal of Medical Electronics and Biological Engineering. vol.30, suppl.343 (1992)

Nogi, K.：“使用鸡蛋测量胚胎心率变异性。（日语）”日本医学电子和生物工程杂志。

長谷川淳: "鶏胚を用いた発生期の心拍数変動シミュレーション" 医用電子と生体工学(特別号). 31. 365 (1993)

长谷川淳：“使用鸡胚胎模拟发育过程中的心率波动”《医疗电子和生物工程》（特刊）31. 365 (1993)。

K.Kobayashi: "Heart Rate Measurementm of chick Embryo for Interpretation of Fetal Heart Rate Variability." Methods of Information in Medicine. 33(掲載予定). 1/94 (1994)

K.Kobayashi：“用于解释胎儿心率变异性的鸡胚心率测量。”医学信息方法 33（即将出版）。