Investigation of interactions between the organelle motion and filamentous structures within cells by cytomatomagnetometry.

通过细胞磁力测量研究细胞内细胞器运动和丝状结构之间的相互作用。

基本信息

批准号：
04452212
负责人：
NEMOTO Iku
金额：
$ 1.73万
依托单位：
Tokyo Denki University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1993
项目状态：
已结题

项目摘要

We studied the feasibility and efficacy of a mangetometric method for investigations of intracellular motions, particularly the possible interactions between phagosome membrane and filamentous strucures within the cell. The present investigation has focued on showing that this method can be a good probe in investiating the elementary processes in phagosome motions. Cytomagnetometry, as we call it, has the following advantages. 1)Intracellular motions within living cells can be measured ; 2)Measurement itself is relatively easy ; 3)Magnetic particles can be manipulated by an external force non-invasively and thus can be subject to a large variety of experiments ; 4)The measurement is done on a large number of cells at a time, resulting in enough averaging.It has now become clear that cytomagnetometry yields data which reflect intracellular motions of phagosomes and by manipulating the magnetic particles within cells one can obtain data which also reflect the rheological properties.Our experiments focused upon the hypothesis of phagosome motion through interaction of actinfilaments and myosin on the phagosome membrane. The results show that actin filaments do play some role in phagosme motion, although the results were not as clear cut and decisive as we had hoped. Experiments performed to see viscocity generation by actin filaments yielded intriguing but interesting results which further suggest actin's involvement in phagosome motions.We also built a mathematical model in which phagosome motions are caused by association of myosin on the phagosome membrane with an actin filament surrounding the phagosome and its decomposition caused by ATP hydrolysis. Association and decomposition were assumed to be Poisson processes and it was shown that it caused a rotationary random walk and also that the association acts as friction to external forces.

我们研究了一种用于研究细胞内运动的曼特测量方法的可行性和功效，尤其是细胞内吞噬体膜与丝状质量之间的可能相互作用。本调查的重点是表明该方法可以很好地调查吞噬体运动的基本过程。我们所说的细胞磁法具有以下优势。 1）可以测量活细胞内的细胞内运动； 2）测量本身相对容易； 3）磁性颗粒可以通过非侵入性来操纵，因此可以进行多种实验； 4）测量一次是对大量细胞进行的，导致足够平均。现在已经清楚地表明，细胞电磁法会产生反映吞噬体的细胞内运动的数据吞噬膜。结果表明，肌动蛋白丝确实在Phagosme运动中发挥了一定作用，尽管结果并不像我们希望的那样明确和果断。进行的实验是通过肌动蛋白丝观察粘多性产生的实验产生了有趣但有趣的结果，这进一步暗示了肌动蛋白参与吞噬体运动。我们还建立了一个数学模型，在该模型中，吞噬体运动是由肌球蛋白在吞噬膜上与吞噬细丝的肌动蛋白在吞噬膜上的结合而引起的，该模型与吞噬型含量及其吞噬作用。假定关联和分解是泊松过程，结果表明它引起了旋转随机行走，并且该关联起到了对外力的摩擦。