EXPERIMENTAL STUDY OF RADIATION-INDUCED DEMENTIA

辐射诱发痴呆的实验研究

基本信息

批准号：
03454346
负责人：
TAKEDA Norio
金额：
$ 2.62万
依托单位：
NIIGATA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1992
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-03454346/
关键词：
Radiation injury Rat Water maze Passive avoidance Metabolism,glucose Myelin basic protein Myelin-associated glycoprotein Myelination 髄鞘形成障害オートラジオグラフィー myelin basic protein myelin-associated glycoprotein glial fibrillary acidic pro

项目摘要

To investigate radiation effects on normal brain in clinical radiotherapy of brain tumor, the effects of X-irradiation on normal brain in both adult and new-born rats have been studied.1. Fischer 344 rats, aged 6 months, received fractionated irradiation (5Gy/day, 2days/week;total dose, 40Gy) to the whole brain. The rats were tested using a water maze task and a passive avoidance task at 6, 9, 12, months after radiation. In comparison with control rats, the irradiated rats showed marked lerning impairment in a passive avoidance task done 12 months after radiation.Local cerebral glucose utilization (LCGU) was mesured by the 14C-2-deoxyglucose method 12 months after radiation. The LCGU of some brain structures was reduced.The rats were sacrificed at 12 months after radiation and examined for histological changes and immunohistochemically. With conventional stains there were no apparent differences between control and irradiatedb rain. A number of GFAP-positive astrocytes were increased in the irradiated brain.2. Neonatal rats, aged 4 to 15 days, received single doses (5, 7.5, 10, 15 Gy) of X-irradiation to the right hemicranium. The rats were sacrificed at 14, 21, 28 and 35 days of age and examined for histological change. With conventional stains, neurons and blood vessels showed no abnormal changes. As compared with unirradiated hemisphere, irradiated hemisphere showed delayed myelination. Immunohistochemistry for myelin basic protein and myelin-associated glycoprotein was sensitive histopathological indicator of radiation effect of oligodendroglias.The delayed myelination was due to direct radiation effect on oligodendroglias.

为了研究脑肿瘤临床放疗对正常大脑的辐射影响，已经研究了X辐照对成人和新生大鼠正常脑的影响。1。 Fischer 344只大鼠，年龄6个月，接受了分离的辐射（5GY/DAY，2天/周；总剂量，40GY）。使用水迷宫任务和辐射后6、9、12的被动避免任务对大鼠进行测试。与对照大鼠相比，辐照大鼠在辐射后12个月完成的被动回避任务中显示出明显的降低损害。局部脑葡萄糖利用率（LCGU）通过14c-2-脱氧葡萄糖方法触发，射门后12个月。减少了一些大脑结构的LCGU。在辐射后12个月处死大鼠，并检查组织学变化和免疫组织化学。对于常规的污渍，对照和辐照b降雨之间没有明显的差异。辐照大脑中的许多GFAP阳性星形胶质细胞增加。2。 4至15天的新生大鼠接受了右半颅骨的单剂量（5、7.5、10、15 Gy）。将大鼠在14、21、28和35天大时被处死，并检查组织学变化。使用常规的污渍，神经元和血管没有异常变化。与未辐照的半球相比，辐照半球显示髓鞘延迟。髓磷脂碱性蛋白和与髓磷脂相关的糖蛋白的免疫组织化学是少突胶质素的辐射效应的敏感组织病理学指标。延迟的髓鞘化是由于直接辐射对寡聚的辐射作用引起的。