EXPERIMENTAL STUDY OF RADIATION-INDUCED DEMENTIA

辐射诱发痴呆的实验研究

基本信息

批准号：
03454346
负责人：
TAKEDA Norio
金额：
$ 2.62万
依托单位：
NIIGATA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1992
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-03454346/
关键词：
Radiation injury Rat Water maze Passive avoidance Metabolism,glucose Myelin basic protein Myelin-associated glycoprotein Myelination 髄鞘形成障害オートラジオグラフィー myelin basic protein myelin-associated glycoprotein glial fibrillary acidic pro

项目摘要

To investigate radiation effects on normal brain in clinical radiotherapy of brain tumor, the effects of X-irradiation on normal brain in both adult and new-born rats have been studied.1. Fischer 344 rats, aged 6 months, received fractionated irradiation (5Gy/day, 2days/week;total dose, 40Gy) to the whole brain. The rats were tested using a water maze task and a passive avoidance task at 6, 9, 12, months after radiation. In comparison with control rats, the irradiated rats showed marked lerning impairment in a passive avoidance task done 12 months after radiation.Local cerebral glucose utilization (LCGU) was mesured by the 14C-2-deoxyglucose method 12 months after radiation. The LCGU of some brain structures was reduced.The rats were sacrificed at 12 months after radiation and examined for histological changes and immunohistochemically. With conventional stains there were no apparent differences between control and irradiatedb rain. A number of GFAP-positive astrocytes were increased in the irradiated brain.2. Neonatal rats, aged 4 to 15 days, received single doses (5, 7.5, 10, 15 Gy) of X-irradiation to the right hemicranium. The rats were sacrificed at 14, 21, 28 and 35 days of age and examined for histological change. With conventional stains, neurons and blood vessels showed no abnormal changes. As compared with unirradiated hemisphere, irradiated hemisphere showed delayed myelination. Immunohistochemistry for myelin basic protein and myelin-associated glycoprotein was sensitive histopathological indicator of radiation effect of oligodendroglias.The delayed myelination was due to direct radiation effect on oligodendroglias.

为探讨脑肿瘤临床放疗中放射线对正常脑组织的影响，本研究采用X线照射对成年及新生大鼠正常脑组织的影响进行了研究。 1． Fischer 344只大鼠，6个月大，接受全脑分次照射（5Gy/天，2天/周；总剂量，40Gy）。在辐射后 6、9、12 个月时，使用水迷宫任务和被动回避任务对大鼠进行测试。与对照大鼠相比，受辐射大鼠在辐射后12个月进行的被动回避任务中表现出明显的学习障碍。辐射后12个月采用14C-2-脱氧葡萄糖法测定局部脑葡萄糖利用率（LCGU）。部分脑结构的LCGU降低。放射后12个月处死大鼠，检查组织学变化和免疫组化。对于常规污渍，对照和辐照雨之间没有明显差异。受照射脑组织中GFAP阳性星形胶质细胞数量增多。2． 4 至 15 天的新生大鼠右半颅接受单剂量（5、7.5、10、15 Gy）X 射线照射。在14、21、28和35天龄时处死大鼠并检查组织学变化。常规染色，神经元和血管未见异常变化。与未受照射的半球相比，受照射的半球表现出髓鞘形成延迟。髓鞘碱性蛋白和髓鞘相关糖蛋白的免疫组织化学是少突胶质细胞辐射效应的敏感组织病理学指标。髓鞘形成延迟是由于辐射对少突胶质细胞的直接作用所致。