Time of development up to the first mitosis

发育至第一次有丝分裂的时间

基本信息

批准号：
03454023
负责人：
YONEDA Mitsuki
金额：
$ 2.37万
依托单位：
KYOTO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1992
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-03454023/
关键词：
DNA synthesis Starfish S-pahse Cell fusion Cell cycle Image analysis BrdU Meiosis 核分裂卵母細胞ブロモウラシル融合核

项目摘要

1. DNA-synthetic period in starfish eggs: Maturing oocytes were fertilized and pulse-labeled with BrdU for detecting S phase in starfish eggs. Fertilized either during or after maturation, eggs entered S-phase before syngamy. In eggs fertilized after maturation, a small area in the zygote nucleus derived probably from the male pronucleus lasted longer to synthesize DNA than the main area. Pattern of BrdU label during S phase was traced and compared with the distribution pattern of chromatin. Image analysis on a computer revealed that the two patterns usually coincide, indicating that DNA synthetic regions in starfish eggs are stretched on a whole length of chromatin.2. Termination of meiotic division in fused oocytes of starfish: Immature oocytes of Asterina pectinifera were fused by treating them with polyethylene glycol following the procedure of Vassetzky et. al. (1986). 1-nethyladenine caused the fused pair to extrude pair of polar bodies (PB1's and PB2's) on schedule of unfused controls, and the pair eventually formed two pronuclei. Two populations of oocytes with different maturation phases were then fused at random. Those pairs that extruded PB2 from one meiotic nucleus within * 8 min of the PB1 extrusion from the other nucleus were observed. In these heterogenous pairs, the PB2 was not formed from the latter nucleus, and both nuclei went through to form pronuclei. Presence of a cytoplasmic factor to stop cell division in maturing oocytes is suggested.

1。海星卵中的DNA合成期：将成熟的卵母细胞受精并用BRDU脉冲标记，以检测海星卵中的S相。在成熟期间或成熟后受精，鸡蛋在合成体前进入了S期。在成熟后受精的卵中，合子核中的一个小面积可能来自雄性原核的持续时间比主要区域更长。 S相期间BRDU标签的模式与染色质的分布模式进行了比较。计算机上的图像分析表明，这两种模式通常重合，表明海星卵中的DNA合成区域在整个染色质上拉伸。2。在融合的卵母细胞中减数分裂分裂的终止：通过通过vassetzky et的手术，用聚乙烯乙二醇处理，通过用聚乙烯乙二醇对其进行融合。 al。（1986）。 1-氯迪尼在未连接的控制仪上使融合对挤出了一对极物体（PB1和PB2），这对最终形成了两个prainuclei。然后将两个具有不同成熟阶段的卵母细胞随机融合。观察到了从另一个核的PB1挤出8分钟内从一个减数分裂核中挤出PB2的对。在这些异源对中，PB2不是由后一个核形成的，并且两个核都形成了原核。建议在成熟的卵母细胞中停止细胞分裂的细胞质因子。