Search for dynamic biochemical markers in visual system.

寻找视觉系统中的动态生化标记。

基本信息

批准号：
02454410
负责人：
MIZUNO Aritake
金额：
$ 3.84万
依托单位：
The Jikei University School of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1994
项目状态：
已结题

项目摘要

The purpose of this project was a search for biochemical markers in visual pathways. It was rather difficult to search these markers under physiological condition such as visual deprivation. We found three biochemical markers under pathological condition. One was uric acid in retina and optic nerve under ischemia. We could measure tissue uric acid by HPLC method. After occlusion of carotid and vertebral arteries of rat for 20 min, the blood flow was reperfused. The elevation of uric acid level showed biphasic profile at 20 min after occlusion and at 60 min after reperfusion. The second elevation must be due to oxygen toxicity by reperfusion. The optic nerve was more affected than the retina. The optic nerve was more susceptible to ischemia than the retina. Another makers were actin and tubulin in frog retina. The frog optic nerve undergo regeneration after optic nerve dissection. We found transient increase in actin and tubulin gene expression after optic nerve dissection in frog but not in rat The third experiment we have done was visual deprived experiment using new born rat. Rats born under complete darkness were divided several groups; keeping in 12 hours cycle dark and light condition, keeping in complete darkness for 3 weeks, and keeping in 3 week darkness and giving 10 or 60 min light stimulation. Fixed frozen section of the brain involving visual cortex and lateral geniculate nucleus were subjected for in situhybridization. However little expression of c-fos and Jun proto-oncogene and no different between animal groups were observed.

该项目的目的是寻找视觉通路中的生化标记。在视觉剥夺等生理条件下寻找这些标记是相当困难的。我们在病理条件下发现了三种生化标志物。一是缺血状态下视网膜和视神经中的尿酸。我们可以通过HPLC方法测量组织尿酸。大鼠颈动脉和椎动脉闭塞20分钟后，血流重新灌注。尿酸水平升高在闭塞后20分钟和再灌注后60分钟呈现双相曲线。第二次升高一定是由于再灌注引起的氧中毒。视神经比视网膜受到的影响更大。视神经比视网膜更容易出现缺血。另一种制造者是青蛙视网膜中的肌动蛋白和微管蛋白。青蛙视神经在视神经解剖后进行再生。我们发现青蛙视神经解剖后肌动蛋白和微管蛋白基因表达短暂增加，而大鼠则没有。我们所做的第三个实验是使用新生大鼠进行视觉剥夺实验。在完全黑暗的环境下出生的老鼠被分成几组；保持12小时循环黑暗和光照条件，保持完全黑暗3周，保持3周黑暗并给予10或60分钟的光刺激。对涉及视觉皮层和外侧膝状核的大脑固定冰冻切片进行原位杂交。然而，c-fos 和 Jun 原癌基因的表达很少，并且动物组之间没有观察到差异。