Research on the relationship between environments and heredity

环境与遗传关系的研究

基本信息

批准号：
01480202
负责人：
HIGURASHI Makoto
金额：
$ 4.42万
依托单位：
Dept. of Maternal and Child Health, Univ. of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1991
项目状态：
已结题

项目摘要

Down syndrome, Klinefelter syndrome, and Recklinghausen disease are hereditary disorders characterized by congenital malformations, and a high risk of malignancy. Cultured lymphocytes from the patients with these disorders showed more chromosomal sensitivity after irradiation or to mitomycin-C than do normal control subjects.The chromosomal sensitivity to mitomycin-C (MMC) and cell-cycle kinetics in cells from patients with Klinefelter syndrome, and Recklinghausen disease were studied by techniques of sisterchromatid exchanges (SCEs). In Klinefelter syndrome the frequencies of MMC-induced SCEs increased in proportion to the increase in MMC, concentration in both patient and normal control cells. At low levels of MMC there were no significant differences in SCE frequencies between the patient and normal control cells, but at MMC concentrations of 3X10^<-8>M (p<0.05) and 1X10^<-7> M (p<0.01), significant increases in the frequency of MMC-induced SCEs were observed in cells from patients compared to cells from normal controls. Although the analysis of cell-cycle kinetics both after various culture times and after treatment with MMC revealed that there were no significant differences between the patient and normal control cells, patients with Klinefelter syndrome showed a tendency to cell-cycle delays after treatment with MMC in comparison with normal controls. In Recklinghausen disease, the sensitivity to MMC showed the heterogeneity in response.

唐氏综合症，克莱恩·费尔特综合症和Recklinghausen疾病是以先天性畸形为特征的遗传疾病，具有高恶性风险。与正常对照受试者相比，来自这些疾病患者的培养的淋巴细胞表现出更大的染色体敏感性或对丝裂霉素-C的染色体敏感性。对丝裂霉素-C（MMC）（MMC）的染色体敏感性和细胞周期动力学的染色体敏感性和klinefelter综合症患者的细胞中的细胞中的细胞中的细胞敏感性通过姐妹进行了姐妹，从而对姐妹进行了研究。在KlineFelter综合征中，MMC诱导的SCE的频率与MMC的增加，患者和正常对照细胞的浓度成比例增加。在低水平的MMC下，患者和正常对照细胞之间的SCE频率没有显着差异，但是在MMC浓度为3x10^<-8> m（P <0.05）和1x10^<-7> m（P <0.01）时，MMC诱导的SCES的频率显着增加，在MMC诱导的SCE中的频率与正常对照组相比。尽管在各种培养时间和使用MMC治疗后对细胞周期动力学的分析表明，患者和正常对照细胞之间没有显着差异，但KlineFelter综合征患者与正常对照组相比，KlineFelter综合征的患者在用MMC治疗后出现细胞周期延迟的趋势。在Recklinghausen病中，对MMC的敏感性显示出反应的异质性。