Deciphering molecular mechanisms controlling seed development under low energy stress

破译低能量胁迫下控制种子发育的分子机制

• 批准号: 408153945
• 负责人: Dr. Christoph Weiste
• 金额: --
• 依托单位: Julius-von-Sachs-Institut für Biowissenschaften
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2018
• 资助国家: 德国
• 起止时间: 2017-12-31 至 2022-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/408153945?language=en
• 关键词: Deciphering; molecular; mechanisms; controlling; seed

Deciphering molecular mechanisms controlling seed development under low energy stressSummary:Seed yield is a major agronomic trait that depends on proper seed development. It is well-established that the early developmental stages, which comprise embryogenesis, are largely controlled by transcriptional regulation mediated by the phytohormone auxin. Besides this, recent studies revealed that environmental stresses that converge on energy deprivation, also exclusively interfere with early seed establishment, resulting in growth repression of siliques and seed abortion. Although this low energy stress (LES) response was characterised to be reversibly and dynamically tuned according to the plants` prevailing energy status, the underlying molecular mechanism is yet unknown.In our previous work, we unravelled that central players of the plant‘s energy management system, namely basic leucine ZIPper 11 (bZIP11) related transcription factors (TF), which are controlled by the pivotal low-energy activated Snf1 Related Protein Kinases 1 (SnRK1s) integrate low-energy related stimuli into auxin-driven meristematic growth processes. This is mechanistically accomplished by controlling expression of specific Aux/IAAs, which constitute negative feedback regulators of auxin signalling, auxin transport and in consequence auxin-driven meristem activity. As bZIP expression was found to result in strongly reduced seed set and bZIP11-related transcripts that underlie energy-dependent posttranscriptional regulation, reveal a distinct accumulation within the early ovule, we assume that a SnRK-bZIP growth regulatory system evolved to adjust auxin-mediated seed development according to the plants‘ prevailing energy reserves. Specifically operating during early and not late developmental stages, this low energy-triggered system could enable channelling of residual energy resources to nearly completed seeds, thereby ensuring survival of plant progeny under LES.To test this hypothesis, we will initially characterize starvation-responsive bZIP expression domains and kinetics throughout embryogenesis. At the defined, bZIP-controlled seed stages, we will study effects of bZIP mis-expression on the embryos’ auxin signalling and morphology under high- and low energy conditions, making use of inducible and constitutive gain- and loss-of-function approaches. The obtained results will unravel the impact of bZIP11-related TFs in starvation-responsive seed development. Finally, we will address the underlying molecular mechanisms by deciphering the transcriptional network downstream of the bZIP regulators, applying a combination of RNAseq and ChIPseq techniques. Taken together, the proposed project will provide essential insights how energy starvation is transduced into seed development. The obtained knowledge will strongly foster basic research on plant stress adaptation processes and, due to its impact on seed yield, support the development of stress tolerant crops.

摩尔机制控制种子低耐应力：种子的产量是一个主要的农艺性状，取决于pro的发育。大约是Allyooth的响应（LES）的响应是反向的，并且动态地调整了植物的预售能量状态，基本的分子机制尚不清楚。管理系统，非基本的亮氨酸拉链11（BZIP11）相关的转录因子（TF），由关键的低能SNF1相关蛋白激酶1（SNRK1S）控制，将低能刺激整合到驱动的阶级生长过程中。通过控制特异性/IaA的表达，该特异性/IaAs的表达是生长素转运的负反馈调节剂，其结果是在强烈减少的集合和BZIP11与Ensip依赖能量的转录本中的ult。 SNRK-BZIP生长系统根据植物的Ergy储量来调整生长素介导的种子发育。 ，从而确保LES下的植物后代的存活。在测试thiththithesissississis ize饥饿反应性BZIP ExpressINS和动力学中，整个胚胎发生了BZIP误差的CTS。功能障碍的结果将在饥饿响应籽元素中的影响能量饥饿被引发到种子发展中。