Molecular mechanism of site-specificity of Caligus fugu on fins of puffer fish

河豚鱼鳍上河豚位点特异性的分子机制

• 批准号: 23658162
• 负责人: OHTSUKA Susumu
• 金额: $ 2.5万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-23658162/
• 关键词: トラフグ類; ウオジラミ類; 宿主特異性; 寄生部位特異性; 鰭; サブトラクティブPCR法; カワハギ類; ペンネラ科; 寄生; サブトラクティブハイブリダイゼーション法; ペンネラ類; 国際情報交換; 「国際情報交換」（連合王国）; トラフグ; PCR; 生活史; 感染; Pseudocaligus fugu; copepodid; フグ; 細胞表面ディスプレイ法; レセプター; リガンド

(1) The life cycle and developmental stages of some parasitic copepods have been newly revealed by the present investigation. The number of post-naupliar stages of caligids is evidently six. Reinfection of adults is implied in some caligids. The whole life cycle of a pennelid Peniculus minuticaudae infecting fins of filefish is totally disclosed: the hatching stage is copepodids; no host switching is found. It is likely that fish fins are feasibly infected by infective stages of parasitic copepods but so limited for fecundity of adult females. (2) Results of bioassay indicate that attractants for the infective copepodid of Caligus fugu is water-soluble. At least 10 genes coding secretory proteins or enzymes were obtained by suppression subtractive hybridization, which was applied to isolate genes expressed higher in fins compared to skin. These are likely to be candidate genes related to the attachment site-specificity of the infective stage.

(1)本次调查新揭示了一些寄生桡足类的生命周期和发育阶段。卡利吉德的无节幼体后阶段的数量显然是六。某些caligids 暗示着成人的再感染。完整揭示了感染锉鱼鳍的潘尼科小尾小尾虾的整个生命周期：孵化阶段是桡足类；未发现主机切换。鱼鳍很可能被寄生桡足类的感染阶段所感染，但成年雌性的繁殖力却非常有限。 (2)生物测定结果表明，河豚感染性桡足类引诱剂是水溶性的。通过抑制消减杂交获得了至少 10 个编码分泌蛋白或酶的基因，该杂交用于分离在鳍中表达高于皮肤的基因。这些可能是与感染阶段的附着位点特异性相关的候选基因。

项目成果

Occurrene of caligid copepods (Crustacea) in plankton samples collected from Japan and Thailand, with the description of a new species

从日本和泰国采集的浮游生物样本中存在桡足类（甲壳纲），并描述了一个新物种

• 发表时间: 2012
• 期刊: Species Diversity
• 作者: B. A. Venmathi Maran;S. Ohtsuka;P. Jitchum
• 通讯作者: P. Jitchum

The caligid life cycle: new evidence from Leophtheirus elegans reconciles the cycles of Caligus and Lepeophtheirus (Copepodoa : Caligidae)

Caligid 的生命周期：来自秀丽隐杆线虫的新证据调和了 Caligus 和 Lepeophtheirus 的周期（Copepodoa ： Caligidae）

• 发表时间: 2013
• 期刊: Parasite
• 影响因子: 2.9
• 作者: B. A. Venmathi Maran;S. Y. Moon;S. Ohtsuka;S.-Y. Oh;H. Y. Soh;J.-G. Myoung;A. Iglikowska;G. A. Boxshall
• 通讯作者: G. A. Boxshall

Candidate key molecule(s) determining host specificity of parasite on fugu, Takifugu rubripes

确定河豚、红鳍东方鲀寄生虫宿主特异性的候选关键分子

• 发表时间: 2012
• 作者: S. Tasumi;A. Yamaguchi;Y. Hirabayashi;S. Kido;K. Kobayashi;W. Kai;S. Hosoya;S. Tsutsui;O. Nakamura;H. Suetake;K. Kikuchi;Y. Suzuki
• 通讯作者: Y. Suzuki

竹原ステーションにおける展開中の研究

竹原站正在进行的研究

トラフグのゲノム育種

虎河豚基因组育种

• 发表时间: 2011
• 作者: 水野初;津山尚宏;原田隆範;升島努;西村善文;鈴木譲・菊池潔
• 通讯作者: 鈴木譲・菊池潔