Is there gonadal stimulation hormone in sea urchins?

海胆中是否含有性腺刺激激素？

• 批准号: 23658152
• 负责人: TAKAGI Yasuaki
• 金额: $ 2.41万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-23658152/
• 关键词: ウニ; 生殖巣; 生殖腺刺激ホルモン; 器官培養; 生体外器官培養; 内分泌; MYP; リアルタイムPCR; ホルモン; エゾバフンウニ; 卵巣; 精巣; 遺伝子微量定量系; 栄養細胞

Sea urchin gonadal growth is characterized by intragonadal nutrient storage and the use of the stored nutrient for gametogenesis. Before gametogenesis initiation, gonads increase in size by accumulating nutrients such as proteins, lipids and carbohydrates in nutritive phagocytes that fill the gonadal lumina of both sexes. The major protein is termed major yolk glycoprotein or major yolk protein (MYP), which is a glycoprotein having molecular weight of 160-180 kDa, and has significant roles in gametogenesis. However, we have no information about gonadal development mechanisms, as well as the regulation mechanism on the synthesis of MYP in gonads. Therefore, to understand the mechanisms of gonadal development, we developed quantitative PCR system of MYP mRNA expression as well as an organ culture systems using sea urchin gonads. These techniques will enable a endocrinological research on gonadal developments in the sea urchin.

海胆性腺的生长的特征是腹养分储存和储存的养分用于配子发生的特征。在配子发生启动之前，通过积累营养素（例如蛋白质，脂质和碳水化合物）来增加营养吞噬细胞中的营养，使性腺大小增加，这些吞噬细胞充满了两性的性腺。主要蛋白质称为主要蛋黄糖蛋白或主要蛋白蛋白（MYP），该蛋白是一种糖蛋白，其分子量为160-180 kDa，并且在配子发生中具有重要作用。但是，我们没有有关性腺发展机制的信息，也没有有关性腺合成的调节机制。因此，为了了解性腺发育的机制，我们开发了使用海胆性腺的MYP mRNA表达和器官培养系统的定量PCR系统。这些技术将对海胆中性腺发展进行内分泌研究。