Rapid Determination of Phytate and Other Inositol Phosphates

快速测定植酸盐和其他肌醇磷酸盐

• 批准号: 01880018
• 负责人: YOSHIDA Tsutomu
• 金额: $ 1.15万
• 依托单位: Tachikawa College of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-01880018/
• 关键词: Phytate; Ion exchange chromatography; Inositol phosphates; Mineral; イノシト-ル リン酸

Sodium phytate added into the diets usually reduce the availability of minerals such as Ca, Mg, Fe, Zn in the gastrointestinal tract of rats. However almost 60% of phytate is hydrolysed in the small intestine and the cecum of rats. Hydrolysis of phytate(IP_6)was usually discussed about amount of phytate left in the rat feces. Effects of inositol penta-(IP_5), tetra(IP_4), tri-(IP_3), di-(IP_2), mono-(IP_1)phosphates on mineral availability in the gastrointestinal tracts have not been discussed, because there is no rapid determination of these inositol phosphatase. The objective of this research was to establish a direct injection method, for phytate and other inositol phosphates determination. An indirect photometric ion chromatographic method using a HPLC system with UV detector(matsunaga, et. al., 1988)was carried out for the determination of phytate and other inositol phosphates. Sodium phytate was boiled in 6N-HCl for 2 hours then evaporated to dryness after washing with H 0. Precipitate was disolved in H 0 and was applied to an anion exchange HPLC system. The inositol hexa-phosphate could be separated from other inositol phosphtes(IP_5 -IP_1). Ther were peaks which assumed to be IP_5, and IP_4-IP_1(sometimes IP_5, IP_4, IP_3-IP_1). They were not identified because no standards were available. Momen and Kinugoshi tofe with different kinds of coagurant were analyzed for IP_6 by using this method. Inositol hexaphosphate in samples of Momen tofu was able to separated and has good analytical values, but not in samples of Kinugoshi tofu.

在饮食中添加的植物钠通常会降低大鼠胃肠道中的矿物质，例如Ca，Mg，Fe，Zn。然而，将近60％的植物植物在小肠和大鼠的小肠中被水解。通常讨论有关大鼠粪便中剩余的植物量的水解（IP_6）。肌醇penta-（IP_5），TETRA（IP_4），TRI-（IP_3），DI-（IP_2），单核 - （IP_1）磷酸盐对胃肠道矿物质的可用性的影响，因为尚未迅速确定这些N insitol磷酸化酶。这项研究的目的是建立一种直接注射方法，用于植酸和其他肌醇磷酸盐的测定。使用HPLC系统具有紫外检测器的间接光度离子色谱法（Matsunaga等，1988）用于测定植酸和其他肌醇磷酸盐。将磷酸钠在6N-HCL中煮沸2小时，然后用H 0洗涤后蒸发至干燥。将沉淀物溶解在H 0中，并将其应用于阴离子交换HPLC系统。肌醇HexA-磷酸盐可以与其他肌醇磷酸盐分离（IP_5 -IP_1）。那是假定为IP_5的峰和IP_4-IP_1（有时IP_5，IP_4，IP_3-IP_1）。由于没有标准，因此未被确定。使用此方法分析了IP_6的Momen和Kinugoshi Tofe，并分析了IP_6。 Momen豆腐样品中的肌醇六磷酸盐能够分离并且具有良好的分析值，但在kinugoshi豆腐样品中却没有。