Modulation of skin microbiota through basememt membrane

通过基底膜调节皮肤微生物群

• 批准号: 25670494
• 负责人: SHIMIZU Hiroshi
• 金额: $ 2.41万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2013
• 资助国家: 日本
• 起止时间: 2013-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-25670494/
• 关键词: 皮膚細菌叢; 表皮水疱症; 皮膚基底膜; 細菌叢

Skin microbiota of mice deficient for skin basement membrane protein (BMZ-deficient mice) were analyzed. At P1, BMZ-deficient mice had less abundant skin microbiota compared with the control group mice while the quantity of skin bacteria was comparable between BMZ-deficient mice and the control mice at P4. Visualization of skin microbiota using FISH methods revealed that bacterial signals were located mainly in the cornified layers of BMZ-deficient mice and the control mice. The specificity of the probe to detect bacterial signals was confirmed by the negative FISH signals using a probe reacting with fungal species which are not found in skin. Further analyses will be done on microbiota of adult BMZ-deficient mice skin and human epidermolysis bullosa patients' skin.

分析了缺乏皮肤基底膜蛋白（BMZ缺陷小鼠）的小鼠的皮肤菌群。在P1时，与对照组小鼠相比，缺乏BMZ的小鼠皮肤菌群较少，而皮肤细菌的数量在缺乏BMZ的小鼠和P4处的对照小鼠之间相当。使用鱼类方法对皮肤菌群的可视化表明，细菌信号主要位于缺乏BMZ的小鼠和对照小鼠的层状层中。探针检测细菌信号的特异性通过负鱼信号证实，使用探针与皮肤中未发现的真菌物种反应。将对成年BMZ缺陷小鼠皮肤和人表皮溶液Bullosa患者皮肤的菌群进行进一步的分析。

项目成果

Shedding of the type VII collagen. The International Pre IID

VII 型胶原蛋白的脱落。

• 发表时间: 2013
• 作者: Cipolat S;Hoste E;Natsuga K;Quist S;Watt FM;Nishie W
• 通讯作者: Nishie W

Repeated skin sampling and prolonged incubation period identified cutaneous Mycobacterium chelonae infection on the face in an immunocompetent man

重复的皮肤取样和延长的潜伏期确定了一名免疫功能正常的男性面部皮肤龟分枝杆菌感染

• DOI: 10.1111/bjd.12638
• 发表时间: 2014
• 期刊: Br J Dermatol
• 作者: Muramatsu K;Nomura T;Ito T;Hamade Y;Hirata Y;Fujita Y;Abe R;Shimizu H
• 通讯作者: Shimizu H

A novel NCSTN mutation alone may be insufficient for the development of familial hidradenitis suppurativa

仅一种新的 NCSTN 突变可能不足以导致家族性化脓性汗腺炎的发生

• DOI: 10.1016/j.jdermsci.2014.01.013
• 发表时间: 2014
• 期刊: J Dermatol Sci
• 影响因子: 4.6
• 作者: Nomura Y;Nomura T;Suzuki S;Takeda M;Mizuno O;Ohguchi Y;Abe R;Murata Y;Shimizu H
• 通讯作者: Shimizu H

Epidermal Barriers

• DOI: 10.1101/cshperspect.a018218
• 发表时间: 2014-04-01
• 期刊: COLD SPRING HARBOR PERSPECTIVES IN MEDICINE
• 影响因子: 5.4
• 作者: Natsuga, Ken

Methods Mol Biol

分子生物学方法

• 发表时间: 2013
• 作者: Ishii;K. Amagai;M.
• 通讯作者: M.