Transomic analysis of adaptation induced by a synthetic gene circuit

合成基因回路诱导适应的转染色体分析

• 批准号: 25640110
• 负责人: ITO Takashi
• 金额: $ 2.58万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2013
• 资助国家: 日本
• 起止时间: 2013-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-25640110/
• 关键词: 人工遺伝子回路; トランスオミクス; 適応; ヒストンメチル化; ミトコンドリア; レトロトランスポゾン; SAM

The cell can adapt to various environmental changes and stably inherit the adapted states to the daughter cells. It has been long argued that such adaptation stems from the plasticity of gene circuits and that mutations play a role in fixation of the state in the population. We used a synthetic gene circuit to expose yeast cells to a selection pressure and found that they can stably maintain an adapted state even after its withdrawal. To address the mechanism underlying this phenomenon, we took a transomic analysis that integratively examines the layers of the genome DNA, chromatin, and RNAs, and revealed evidence for potential involvement of movable genetic elements, histone modifications and mitochondria.

细胞可以适应各种环境变化，并将适应的状态稳定地遗传给子细胞。长期以来，人们一直认为这种适应源于基因回路的可塑性，并且突变在群体状态的固定中发挥着作用。我们使用合成基因回路将酵母细胞暴露在选择压力下，发现即使在退出后，它们也能稳定地保持适应状态。为了解决这一现象背后的机制，我们进行了转染色体分析，综合检查了基因组 DNA、染色质和 RNA 的各层，并揭示了可移动遗传元件、组蛋白修饰和线粒体潜在参与的证据。

项目成果

人工遺伝子回路によって誘導される適応現象のトランスオミクス解析

人工基因电路引起的适应性现象的跨组学分析

• 发表时间: 2013
• 作者: 梅山大地;伊藤隆司
• 通讯作者: 伊藤隆司