Pathological Significance of modified nucleic acid base in human tissue.

人体组织中修饰核酸碱基的病理意义。

• 批准号: 24659161
• 负责人: SUGIMURA Haruhiko
• 金额: $ 2.5万
• 依托单位: Hamamatsu University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24659161/
• 关键词: DNA 付加体; 修飾核酸; 分子疫学; etheno DNA adduct; demethylation; TET; TDG; IDH; 塩基修復; DNA付加体; DNA修飾; 発がん; 修復遺伝子; 胃がん; 人体病理

Adductomics analysis of alkane substituted exocyclic etheno DNA adducts in human stomach mucosa having gastric cancer was performed for the cases from Lujiang County, Anhui, China, and from Hamamatsu, Japan. Discrimination analysis using quantitative value of 7 lipid per oxidation derived DNA adducts revealed that two regions showed distinctive adductome profile. 3 of 7 DNA adducts in this category were greater in Japanese gastric mucosa than Chinese gastric mucosa, which may suggest gastric carcinogenesis in Japan favor inflammatory or metabolic background. Furthermore, we quantitated 5'-hydroxymethyl deoxycytidine, and its derivatives carboxyl deoxycytidine, and formyl deoxycytidine in gastric cancer and adjacent non-tumor tissues and found the relationship between TET2 expression and 5hmdC. In this attempt we could not identify the adducts specifically high in endemic area of gastric cancer (Lujiang), thus further investigation is necessary.

对中国安徽省庐江县和日本滨松市的胃癌患者胃粘膜中的烷烃取代的环外乙烯 DNA 加合物进行加合物组学分析。使用每个氧化衍生的 DNA 加合物的 7 个脂质的定量值进行的区分分析表明，两个区域显示出独特的加合物谱。此类 7 个 DNA 加合物中的 3 个在日本胃粘膜中比中国胃粘膜中含量更高，这可能表明日本的胃癌发生有利于炎症或代谢背景。此外，我们还定量了胃癌及癌旁非肿瘤组织中的5'-羟甲基脱氧胞苷及其衍生物羧基脱氧胞苷和甲酰基脱氧胞苷，发现了TET2表达与5hmdC之间的关系。本次尝试未能鉴定出胃癌高发区（庐江）的加合物，因此有必要进一步研究。

项目成果

Internal frontier: the pathophysiology of the small intestine.

内部前沿：小肠的病理生理学。

• DOI: 10.3748/wjg.v19.i2.161
• 发表时间: 2013
• 期刊: World J Gastroenterol.
• 作者: Kurabe N;Sugimura H et al.;Sugimura H and Osawa S
• 通讯作者: Sugimura H and Osawa S

Towards the genetics and genomics of diffuse gastric cancer

弥漫性胃癌的遗传学和基因组学

• 发表时间: 2013
• 作者: Hiromi Sakamoto;Norihisa Saeki;Hidemi Itoh;Keitaro Matsuo;Hidetaka Yamada;Haruhiko Sugimura;Hitoshi Ichikawa;Hiroki Sasaki;Teruhiko Yoshida
• 通讯作者: Teruhiko Yoshida

ヒトアダクトームについて

关于人类内合组

• 作者: Hiromi Sakamoto;Haruhiko Sugimura et al.;椙村春彦他;Sugimura H;Sugimura H et al.;椙村春彦
• 通讯作者: 椙村春彦

DNA Adductome, an ultimate exposome of human tissue.

DNA 加合物组，人体组织的终极暴露组。

• 发表时间: 2012
• 作者: Hiromi Sakamoto;Haruhiko Sugimura et al.;椙村春彦他
• 通讯作者: 椙村春彦他

Breakages at YWHAE, FAM22A, and FAM22B loci in uterine angiosarcoma : a case report with immunohistochemical and genetic analysis

子宫血管肉瘤中 YWHAE、FAM22A 和 FAM22B 基因座的断裂：免疫组织化学和遗传分析的病例报告

• DOI: 10.1016/j.prp.2013.09.009
• 发表时间: 2014
• 期刊: Pathol Res Pract
• 影响因子: 2.8
• 作者: Suzuki S;Sugimura H et al.
• 通讯作者: Sugimura H et al.