The molecular mechanism of nuclear pore proteins and leukemogenesis

核孔蛋白与白血病发生的分子机制

• 批准号: 24650611
• 负责人: WONG W・RICHARD
• 金额: $ 2.5万
• 依托单位: Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24650611/
• 关键词: 核膜孔複合体; Rae1; Nup98; JARID1A; 白血病; エピジェネティクス; トランスジェニックマウス; 転写因子

Nuclear pore complexes are embedded in the nuclear envelope and act as molecular sieves, selectively facilitating the transport of proteins and RNA in and out of the nucleus. Recently, we showed that NUP98 binding partner, RAE1 involved in the development of NUP98-HOXA9 leukemogenesis. We want to investigate epigenetic mechanism in carcinogenesis through a novel approach of nucleoporins-histone modifiers orchestration. We also plan to compare the difference between the difference of NUP98 fusions, HOX genes (NUP98-HOXA9) and those fusions with PHD domain (NUP98-JARID1A). Through this grant support, we have generated NUP98-JARID1A mice. We are still investigating the NUP98-JARID1A and the leukemogenesis via this mice. Meanwhile, we also published several related NPC papers and presented some of our data in the international and domestic conferences.

核孔复合物嵌入核包膜中并充当分子筛，有选择地促进蛋白质和RNA进出核的转运。最近，我们表明NUP98结合伴侣RAE1参与NUP98-HOXA9白血病的发展。我们希望通过一种新型的核孔蛋白 - 酮修饰仪编排的方法来研究癌变中的表观遗传机制。我们还计划比较NUP98融合，HOX基因（NUP98-HOXA9）的差异与与PHD域（NUP98-JARID1A）的融合之间的差异。通过这种赠款支持，我们产生了NUP98-JARID1A小鼠。我们仍在研究NUP98-JARID1A和通过这只小鼠的白血病发生。同时，我们还发表了几篇相关的NPC论文，并在国际和国内会议上介绍了我们的一些数据。

项目成果

Nucleoporin Nup98 mediates galectin-3 nuclear-cytoplasmic trafficking.

• DOI: 10.1016/j.bbrc.2013.03.052
• 发表时间: 2013-04-26
• 期刊: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
• 影响因子: 3.1
• 作者: Funasaka, Tatsuyoshi;Balan, Vitaly;Raz, Avraham;Wong, Richard W.
• 通讯作者: Wong, Richard W.

ヌクレオポリンNup62 の有糸分裂期での役割

核孔蛋白 Nup62 在有丝分裂过程中的作用

• 作者: Ohnishi H;Tochio H;Kato Z;Kawamoto N;Kimura T;Kubota K;Yamamoto T;Funasaka T;Nakano H;Wong RW;Shirakawa M;Kondo N.;Richard Wong;Richard Wong;藻寄 茜;遠藤 葵;橋爪 智恵子;山越 奈々;Yamakoshi N;Kobayashi A;Tsuka E;Richard Wong;Richard Wong;Richard Wong;Richard Wong;橋爪 智恵子;Wong RW;藻寄 茜;山越 奈々;橋爪 智恵子;遠藤 葵;Richard Wong;Richard Wong;Richard Wong;藻寄 茜;船坂 龍善;塚 恵利子;橋爪 智恵子;山越 奈々;藻寄 茜
• 通讯作者: 藻寄 茜

Nucleoporin Nup358/RanBP2 associates with Karyopherinβ1 to orchestrate accurate chromosome segregation in mitosis

核孔蛋白 Nup358/RanBP2 与核传递蛋白β1 结合以协调有丝分裂中准确的染色体分离

• 发表时间: 2012
• 作者: Ohnishi H;Tochio H;Kato Z;Kawamoto N;Kimura T;Kubota K;Yamamoto T;Funasaka T;Nakano H;Wong RW;Shirakawa M;Kondo N.;Richard Wong;Richard Wong;藻寄 茜;遠藤 葵;橋爪 智恵子;山越 奈々;Yamakoshi N;Kobayashi A;Tsuka E;Richard Wong;Richard Wong;Richard Wong
• 通讯作者: Richard Wong

ヌクレオポリンNup107 の有糸分裂期での役割

核孔蛋白 Nup107 在有丝分裂过程中的作用

• 作者: Ohnishi H;Tochio H;Kato Z;Kawamoto N;Kimura T;Kubota K;Yamamoto T;Funasaka T;Nakano H;Wong RW;Shirakawa M;Kondo N.;Richard Wong;Richard Wong;藻寄 茜;遠藤 葵;橋爪 智恵子;山越 奈々;Yamakoshi N;Kobayashi A;Tsuka E;Richard Wong;Richard Wong;Richard Wong;Richard Wong;橋爪 智恵子;Wong RW;藻寄 茜;山越 奈々;橋爪 智恵子;遠藤 葵;Richard Wong;Richard Wong;Richard Wong;藻寄 茜;船坂 龍善;塚 恵利子;橋爪 智恵子;山越 奈々
• 通讯作者: 山越 奈々

核膜孔複合体因子Nup62の有糸分裂期における働き

核孔复合因子Nup62在有丝分裂过程中的功能

• 作者: Ohnishi H;Tochio H;Kato Z;Kawamoto N;Kimura T;Kubota K;Yamamoto T;Funasaka T;Nakano H;Wong RW;Shirakawa M;Kondo N.;Richard Wong;Richard Wong;藻寄 茜;遠藤 葵;橋爪 智恵子;山越 奈々;Yamakoshi N;Kobayashi A;Tsuka E;Richard Wong;Richard Wong;Richard Wong;Richard Wong;橋爪 智恵子;Wong RW;藻寄 茜
• 通讯作者: 藻寄 茜