Construction of systematic tools forgenome analysesto determinegenes responsible for various disease model in rats

构建用于基因组分析的系统工具以确定导致大鼠各种疾病模型的基因

• 批准号: 24650238
• 负责人: IMOTO Issei
• 金额: $ 2.5万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24650238/
• 关键词: リサーチバイオリソース; ラット疾患モデル動物; エクソーム; 次世代シーケンサー; 責任遺伝子変異; ゲノム情報; 連鎖解析; ラット; 疾患モデル動物

In order to identify genes responsible for phenotypes observed in disease-model rats and determine molecular mechanisms producing those phenotypes, we modified our self-made rat exon capture system, which can easily analyze larger sets of exons in a liquid phase manner. We constructed an effective pipeline to analyze next-generation sequencing data with larger information of polymorphism and gene annotation data in rats. Through combining those systems with linkage analyses, we constructed effective and systematic tools for rat genome analysis. We finally applied this tool to a newly established rat model with unique phenotype, and validated usefulness of our system by collecting candidate variations possibly responsible for its phenotype.

为了鉴定与疾病模型大鼠中观察到的表型有关的基因并确定产生这些表型的分子机制，我们修改了自制的大鼠外显子捕获系统，该系统可以轻松地以液相方式分析更大的外显子组。我们构建了一个有效的流程来分析具有更大信息的大鼠多态性和基因注释数据的下一代测序数据。通过将这些系统与连锁分析相结合，我们构建了有效且系统的大鼠基因组分析工具。我们最终将该工具应用于新建立的具有独特表型的大鼠模型，并通过收集可能对其表型负责的候选变异来验证我们系统的有用性。

项目成果

徳島大学医学部人類遺伝学分野

德岛大学医学部人类遗传学系

Genetic variations in primary breast cancer and its metastatic tumor.

原发性乳腺癌及其转移性肿瘤的遗传变异。

• 发表时间: 2012
• 作者: Hosoda F;et al.
• 通讯作者: et al.

SIX1 promotes epithelial-mesenchymal transition in colorectal cancer through ZEB1 activation

• DOI: 10.1038/onc.2011.646
• 发表时间: 2012-11-01
• 期刊: ONCOGENE
• 影响因子: 8
• 作者: Ono, H.;Imoto, I.;Inazawa, J.
• 通讯作者: Inazawa, J.

DNA Methylation Signatures of Peripheral Leukocytes in Schizophrenia

• DOI: 10.1007/s12017-012-8198-6
• 发表时间: 2013-03-01
• 期刊: NEUROMOLECULAR MEDICINE
• 影响因子: 3.5
• 作者: Kinoshita, Makoto;Numata, Shusuke;Ohmori, Tetsuro
• 通讯作者: Ohmori, Tetsuro

Stabilization of phenotypic plasticity through mesenchymal-specific DNA hypermethylation in cancer cells

• DOI: 10.1038/onc.2011.373
• 发表时间: 2012-04-01
• 期刊: ONCOGENE
• 影响因子: 8
• 作者: Kurasawa, Y.;Kozaki, K.;Inazawa, J.
• 通讯作者: Inazawa, J.