Exploring natural analogues of future oceans to determine the adaptive potential of marine fishes to climate change

探索未来海洋的自然类似物，以确定海洋鱼类对气候变化的适应潜力

• 批准号: 22KJ3084
• 负责人: Izumiyama Michael
• 金额: $ 1.6万
• 依托单位: Okinawa Institute of Science and Technology Graduate University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2023
• 资助国家: 日本
• 起止时间: 2023-03-08 至 2025-03-31
• 项目状态: 未结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-22KJ3084/
• 关键词: environmental DNA; CO2 seep; climate change

During this fiscal year we conducted a sampling trip in collaboration with the researchers from the IRD (Research Institute for Development, France) collected fishes from mangrove lagoon in Borake New Caledonoia. This site is subjected to low pH during the outgoing tide which simulate predicted conditions of OA by the end of the century. We collected five species for a total of 149 fishes. The species collected include three species of damselfish Dascyllus aruanus, Chromis viridis, Pomacentrus moluccensis, and two species of cardinal fish Zoramia leptacantha, and Zoramia viridiventer. RNA was extracted from the brain and gills of the samples and sequenced and we are currently working on the data analysis. We also extracted DNA from 136 fin clips of Pomacentrus coelestis collected in and around a CO2 seep in Shikine, Japan to examine the population structure of fishes found at the seep. Results from environmental DNA (eDNA) collected at the Shikine CO2 seep was presented at the Australia & New Zealand Environmental DNA Conference. Hobart, Tasmania February 2023.

在这个财政年度，我们与IRD（法国发展研究所）的研究人员合作进行了一次抽样旅行，从新喀里多亚的Mangoon Lagoon收集了鱼类。在即将发出的潮汐期间，该地点的pH值较低，这模拟了预测到本世纪末的OA条件。我们收集了五种物种，共有149种鱼类。收集到的物种包括三种daSsellish dascylis aruanus，Chromis viridis，pomacentrus moluccensis，以及两种红衣主教鱼类琐菌leptacantha和琐罗亚氏菌。从样品的大脑和ill中提取RNA并进行了测序，我们目前正在研究数据分析。我们还从日本Shikine的二氧化碳渗水中及其周围收集的136个FIN片片中提取了DNA，以检查渗水发现的鱼类的种群结构。在澳大利亚和新西兰环境DNA会议上提出了在Shikine CO2渗水上收集的环境DNA（EDNA）的结果。霍巴特，塔斯马尼亚州2023年2月。

项目成果

Community shifts at volcanic CO2 seeps, an eDNA prospective

火山二氧化碳渗漏处的群落变化，eDNA 的展望

• 发表时间: 2023
• 作者: Huang Tsung-Yen;Hirota Masato;Sasaki Daiki;Kalra Rajkumar Singh;Chien Hsiao-Chiao;Tamai Miho;Sarkar Shukla;Mi Yang;Miyagi Mio;Seto Yu;Ishikawa Hiroki;Yang Mi;丹上麻里江;丹上麻里江;戸塚史織;戸塚史織;戸塚史織;玉城福子;玉城福子;玉城 福子;Michael Izumiyama
• 通讯作者: Michael Izumiyama