Influence of ionic liquids on enzyme-catalyzed reactions

离子液体对酶催化反应的影响

基本信息

批准号：
282610332
负责人：
Privatdozent Dr.-Ing. Christoph Held
金额：
--
依托单位：
Lehrstuhl für Thermodynamik (TH)
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2015
资助国家：
德国
起止时间：
2014-12-31 至 2021-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/282610332?language=en
关键词：
Influence ionic liquids enzyme catalyzed

项目摘要

Enzyme-catalyzed reactions become more and more important for the development of new alternative synthetic routes in biotechnological processes. However, the reaction equilibrium of many enzyme-catalyzed reactions is often far on the side of the substrates, since the conversion is thermodynamically limited. In such equilibrium-limited reactions, the substrate concentrations are typically increased in order to maximize yields. However, this method is often not or only partially applicable due to low solubility of hydrophobic substrates in aqueous reaction media. The conversion of equilibrium limited enzymatic reactions can be optimized by the presence of ionic liquids (ILs) in the reaction medium. In this proposal ILs will be used as additives to aqueous reaction media in order to shift the reaction equilibrium to the product side. In addition, substrate solubilities can significantly be increased upon addition of ILs in the reaction medium, thus conversion and selectivity of enzyme-catalyzed reactions can be systematically improved. Furthermore, the ILs can affect the catalytic enzyme activity. For investigations of the reaction equilibrium the enzyme activity has to be ensured under the respective reaction conditions (temperature, pH, IL-concentration). Individual studies of all three effects already exist in the literature, however so far they have only been considered separately for a low number of reactions and not systematically. This research project will contribute by a systematic thermodynamic study of these phenomena (reaction equilibrium, substrate solubility, enzyme activity) to a better understanding of the influence of ILs on enzyme-catalyzed reactions. These properties will be determined experimentally; reaction equilibria and substrate solubilities will also be predicted with a thermodynamic model. The enzyme activity is tested for the respective reaction conditions in order to verify the attainment of thermodynamic equilibrium. For a better understanding of the IL-effect on the enzyme activity the effect of ILs on the thermodynamic stability and on the secondary structure of the enzymes of the investigated reactions will be measured.In order to achieve general knowledge, two different enzyme-catalyzed reactions are considered, each having industrial relevance. In addition, four ILs are studied with respect to their influence on the equilibrium of enzyme-catalyzed reactions, substrate solubility and enzyme activity. These ILs differ in basicity / hydrophobicity / molar mass in order to investigate basically the influence of these properties on reaction equilibrium, substrate solubility and enzyme activity.This research project will contribute significantly to the understanding of the influence of additives of different chemical natures on the thermodynamics of enzyme-catalyzed reactions. In addition, there will be specific quantitative results on the use of ILs as additives for biological reactions.

酶催化的反应对于生物技术过程中新的替代合成途径的发展变得越来越重要。但是，由于转化率在热力学上有限，因此许多酶催化反应的反应平衡通常在底物的一侧。在这种平衡限制的反应中，底物浓度通常会增加，以最大化产量。但是，由于水反应培养基中疏水性底物的溶解度较低，这种方法通常不适合或仅部分适用。平衡有限的酶促反应的转化可以通过反应培养基中的离子液体（ILS）的存在来优化。在此提案中，ILS将用作水反应介质的添加剂，以将反应平衡转移到产物侧。另外，在反应介质中添加IL时，底物溶解度可以显着增加，因此可以系统地改善酶催化反应的转化和选择性。此外，ILS可以影响催化酶活性。为了研究反应平衡，必须在各自的反应条件下（温度，pH，IL浓度）确保酶活性。文献中已经存在所有三种影响的个体研究，但是到目前为止，它们仅针对低的反应而不是系统地考虑了它们。该研究项目将通过对这些现象（反应平衡，底物溶解度，酶活性）的系统热力学研究做出贡献，以更好地理解ILS对酶催化反应的影响。这些特性将通过实验确定；反应平衡和底物溶解度也将通过热力学模型预测。为了验证热力学平衡的达到，测试了各自的反应条件的酶活性。为了更好地了解IL对酶活性的IL效应，将测量ILS对热力学稳定性和对所研究反应酶的二级结构的影响。为了实现通用知识，考虑了两种不同的酶催化反应，考虑了每种酶催化反应，每种反应都具有工业相关性。此外，研究了四个IL关于它们对酶催化反应，底物溶解性和酶活性的影响的影响。这些IL在碱度 /疏水性 /摩尔质量方面有所不同，以便研究这些特性对反应平衡，底物溶解度和酶活性的影响。这项研究项目将显着促进对不同化学方法的添加剂对酶热力学的影响的影响。此外，将使用IL作为生物反应的添加剂将有特定的定量结果。

项目成果

期刊论文数量（6）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Oil desulfurization using deep eutectic solvents as sustainable and economical extractants via liquid-liquid extraction: Experimental and PC-SAFT predictions

DOI：
10.1016/j.fluid.2018.03.018
发表时间：
2018-07
期刊：
Fluid Phase Equilibria
影响因子：
2.6
作者：
Samah E. E. Warrag-Samah-E.-E.-Warrag-31880789;Clarissa Pototzki;N. R. Rodriguez;M. van Sint Annaland;M. Kroon;C. Held;G. Sadowski;C. Peters
通讯作者：
Samah E. E. Warrag-Samah-E.-E.-Warrag-31880789;Clarissa Pototzki;N. R. Rodriguez;M. van Sint Annaland;M. Kroon;C. Held;G. Sadowski;C. Peters

Measuring and Predicting Thermodynamic Limitation of an Alcohol Dehydrogenase Reaction