Influence of ionic liquids on enzyme-catalyzed reactions

离子液体对酶催化反应的影响

基本信息

批准号：
282610332
负责人：
Privatdozent Dr.-Ing. Christoph Held
金额：
--
依托单位：
Lehrstuhl für Thermodynamik (TH)
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2015
资助国家：
德国
起止时间：
2014-12-31 至 2021-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/282610332?language=en
关键词：
Influence ionic liquids enzyme catalyzed

项目摘要

Enzyme-catalyzed reactions become more and more important for the development of new alternative synthetic routes in biotechnological processes. However, the reaction equilibrium of many enzyme-catalyzed reactions is often far on the side of the substrates, since the conversion is thermodynamically limited. In such equilibrium-limited reactions, the substrate concentrations are typically increased in order to maximize yields. However, this method is often not or only partially applicable due to low solubility of hydrophobic substrates in aqueous reaction media. The conversion of equilibrium limited enzymatic reactions can be optimized by the presence of ionic liquids (ILs) in the reaction medium. In this proposal ILs will be used as additives to aqueous reaction media in order to shift the reaction equilibrium to the product side. In addition, substrate solubilities can significantly be increased upon addition of ILs in the reaction medium, thus conversion and selectivity of enzyme-catalyzed reactions can be systematically improved. Furthermore, the ILs can affect the catalytic enzyme activity. For investigations of the reaction equilibrium the enzyme activity has to be ensured under the respective reaction conditions (temperature, pH, IL-concentration). Individual studies of all three effects already exist in the literature, however so far they have only been considered separately for a low number of reactions and not systematically. This research project will contribute by a systematic thermodynamic study of these phenomena (reaction equilibrium, substrate solubility, enzyme activity) to a better understanding of the influence of ILs on enzyme-catalyzed reactions. These properties will be determined experimentally; reaction equilibria and substrate solubilities will also be predicted with a thermodynamic model. The enzyme activity is tested for the respective reaction conditions in order to verify the attainment of thermodynamic equilibrium. For a better understanding of the IL-effect on the enzyme activity the effect of ILs on the thermodynamic stability and on the secondary structure of the enzymes of the investigated reactions will be measured.In order to achieve general knowledge, two different enzyme-catalyzed reactions are considered, each having industrial relevance. In addition, four ILs are studied with respect to their influence on the equilibrium of enzyme-catalyzed reactions, substrate solubility and enzyme activity. These ILs differ in basicity / hydrophobicity / molar mass in order to investigate basically the influence of these properties on reaction equilibrium, substrate solubility and enzyme activity.This research project will contribute significantly to the understanding of the influence of additives of different chemical natures on the thermodynamics of enzyme-catalyzed reactions. In addition, there will be specific quantitative results on the use of ILs as additives for biological reactions.

酶催化反应对于生物技术过程中新的替代合成路线的开发变得越来越重要。然而，许多酶催化反应的反应平衡通常远远偏向底物一侧，因为转化率在热力学上是有限的。在此类平衡限制反应中，通常增加底物浓度以使产量最大化。然而，由于疏水底物在水性反应介质中的溶解度低，该方法通常不适用或仅部分适用。平衡限制酶促反应的转化可以通过反应介质中离子液体 (IL) 的存在来优化。在该提案中，离子液体将用作水性反应介质的添加剂，以使反应平衡向产物侧移动。此外，在反应介质中添加ILs可以显着增加底物溶解度，从而可以系统地提高酶催化反应的转化率和选择性。此外，IL 可以影响催化酶的活性。为了研究反应平衡，必须在各自的反应条件（温度、pH、IL 浓度）下确保酶活性。文献中已经存在对这三种效应的单独研究，但到目前为止，它们仅针对少量反应进行单独考虑，而不是系统地考虑。该研究项目将通过对这些现象（反应平衡、底物溶解度、酶活性）进行系统的热力学研究，有助于更好地了解离子液体对酶催化反应的影响。这些特性将通过实验确定；反应平衡和底物溶解度也将通过热力学模型进行预测。在各自的反应条件下测试酶活性，以验证热力学平衡的实现。为了更好地了解 IL 对酶活性的影响，将测量 IL 对所研究反应的热力学稳定性和酶的二级结构的影响。为了获得一般知识，两种不同的酶催化反应被考虑，每一个都具有工业相关性。此外，还研究了四种离子液体对酶催化反应平衡、底物溶解度和酶活性的影响。这些离子液体的碱度/疏水性/摩尔质量不同，以便从根本上研究这些特性对反应平衡、底物溶解度和酶活性的影响。该研究项目将有助于了解不同化学性质的添加剂对反应平衡的影响。酶催化反应的热力学。此外，使用离子液体作为生物反应添加剂也会有具体的定量结果。

项目成果

期刊论文数量（6）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Oil desulfurization using deep eutectic solvents as sustainable and economical extractants via liquid-liquid extraction: Experimental and PC-SAFT predictions

DOI：
10.1016/j.fluid.2018.03.018
发表时间：
2018-07
期刊：
Fluid Phase Equilibria
影响因子：
2.6
作者：
Samah E. E. Warrag-Samah-E.-E.-Warrag-31880789;Clarissa Pototzki;N. R. Rodriguez;M. van Sint Annaland;M. Kroon;C. Held;G. Sadowski;C. Peters
通讯作者：
Samah E. E. Warrag-Samah-E.-E.-Warrag-31880789;Clarissa Pototzki;N. R. Rodriguez;M. van Sint Annaland;M. Kroon;C. Held;G. Sadowski;C. Peters

Measuring and Predicting Thermodynamic Limitation of an Alcohol Dehydrogenase Reaction