Molecular identification of the transcriptional activator of carotenoid biosynthesis in the fungus Fusarium fujikuroi

真菌藤黑镰刀菌类胡萝卜素生物合成转录激活因子的分子鉴定

基本信息

批准号：
281458954
负责人：
Dr. Steffen Nordzieke
金额：
--
依托单位：
Dep. of Genetics
依托单位国家：
德国
项目类别：
Research Fellowships
财政年份：
2015
资助国家：
德国
起止时间：
2014-12-31 至 2016-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/281458954?language=en
关键词：
Molecular identification transcriptional activator carotenoid

项目摘要

Carotenoids include over 600 different chemical compounds, bearing a large variety of different characteristics. Many of them are key elements of secondary metabolism of bacteria, plants, and fungi. Due to their individual abilities, they provide a large subset of molecular tasks in protecting individuals against light induced or oxidative damage, which reflects their importance for all classes of eukaryotic life. Animals and humans are unable to produce carotenoids. Nevertheless, they are prerequisites for the formation of Vitamin A, and for the protection of the macula lutea. According to this, humans have to incorporate a sufficient amount of carotenoids through their diets. An undersupply, which is common in developing countries, leads to severe defects in vision, skin composition, and the immune systemFungal systems provide an excellent source for the industrial production of natural carotenoids, thereby avoiding biological inactive isomers which are typical byproducts of chemical synthesis. Additionally, fungi need few requirements, are easy to cultivate and, due to their fermentability, can produce enormous amounts of secondary metabolites. To optimize the industrial production, detailed knowledge about the composition and regulation of fungal carotenoid biosynthesis is important.The plant pathogen Fusarium fujikuroi serves as a fungal model system to study carotenoid biosynthesis since the 1980´s. Due to the already performed research of the host group, the structural genes for all proteins involved in the synthesis of carotenoids are known, whereas the regulation of these genes remains unclear. The aim of this project is to characterize novel regulatory proteins of the carotenoid pathway, such as the predicted CarC, a yet undescribed positive regulator of carotenoid biosynthesis expected to interact with the already described negative regulator CarS. For this, Co Immunoprecipitation experiments, pull-down assays, and a heterologous expression in S. cerevisiae will be utilized, aiming at specific characteristics attributed to CarC. These experiments will lead to a credible list of potential candidates. Genetic deletions of the corresponding genes, followed by in depth analyses of the resulting phenotypes, will serve as control experiments and, simultaneously, provide detailed insights also into potential secondary roles of CarC.Already having a strong background in the field of protein-protein interaction and fungal protein complexes, the applicant is highly qualified to perform the targeted experiments. As the host group is one of the leading facilities in the research on F. fujikuroi and has already characterized the structural genes needed for the carotenoid biosynthesis, the collaboration will close the last scientific gap left in the investigation of the regulation. The obtained results may contribute to optimize the industrial production of carotenoids in fungi, further preventing a Vitamin A undersupply of the population.

类胡萝卜素包括600多种不同的化合物，具有各种不同的特征。其中许多是细菌，植物和真菌的继发代谢的关键要素。由于他们的个人能力，它们提供了大量的分子任务，以保护个体免受诱导的光或氧化损害的影响，这反映了它们对所有类别的真核生活的重要性。动物和人类无法产生类胡萝卜素。然而，它们是形成维生素A的先决条件，也是保护黄斑lutea的前提。据此，人类必须通过饮食融入足够数量的类胡萝卜素。在发展中国家中很常见的供应供应会导致视力，皮肤成分和免疫系统的严重缺陷，从而为自然类胡萝卜素的工业生产提供了一种绝佳的来源，从而避免了典型的化学物质副产物的生物非活性异构体。此外，真菌需要很少的需求，易于培养，并且由于其发酵性，可以产生增强量的二级代谢产物。为了优化工业生产，有关真菌类胡萝卜素生物合成的组成和调节的详细知识很重要。自1980年代以来，植物病原体富士馆是一种研究类胡萝卜素生物合成的真菌模型系统。由于宿主组已经进行了研究，已知所有参与类胡萝卜素合成的蛋白质的结构基因，而这些基因的调节尚不清楚。该项目的目的是表征类胡萝卜素途径的新型调节蛋白，例如预测的CARC，这是一种未描述的类胡萝卜素生物合成的阳性调节剂，预计将与已经描述的负调节器相互作用。为此，将利用CO免疫沉淀实验，下拉测定法和酿酒酵母中的异源表达，旨在归因于归因于CARC的特定特征。这些实验将导致潜在候选人的可靠清单。相应基因的遗传缺失，然后对所得表型进行深入分析，将作为控制实验，简单地提供了对CARC的潜在次要作用的详细见解。在蛋白质 - 蛋白质相互作用和真菌蛋白络合物领域具有强大的背景，该领域具有很强的背景，适用于适用于验证目标实验的符合条件。由于寄主组是F. vujikuroi研究的主要设施之一，并且已经表征了类胡萝卜素生物合成所需的结构基因，因此该协作将缩小调查调查中剩下的最后一条科学差距。获得的结果可能有助于优化真菌中类胡萝卜素的工业生产，从而进一步阻止了人群的维生素A供应。