Molecular genetic analysis of hindlimb development in zebrafish

斑马鱼后肢发育的分子遗传学分析

• 批准号: 263801744
• 负责人: Professor Dr. Gerrit Begemann
• 金额: --
• 依托单位: Lehrstuhl für Tierphysiologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2014
• 资助国家: 德国
• 起止时间: 2013-12-31 至 2018-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/263801744?language=en
• 关键词: Molecular; genetic; analysis; hindlimb; development

Despite the complexity of human congenital limb formations and their relative abundance, the molecular mechanisms underlying and specific to pelvic fin/hindlimb formation in the zebrafish have largely been unexplored. We propose to unravel the molecular regulation of zebrafish pelvic fin/hindlimb formation using various genetic approaches. To this end, (I) we will clone and examine a novel mutant with severe defects in the formation of pelvic fins and associated endoskeleton, (II) we will test the hypothesis that RA signaling is required for pelvic fin initiation and will examine an unexpected involvement in patterning the pelvic fin endoskeleton, and (III) we will develop transgenic tools that allow us to manipulate RA signaling, and ultimately any signaling pathway, in developing pelvic fins in a tissue-specific manner and at any given time. Together, our work should provide new and significant insights into the regulation of vertebrate hindlimb development. Our findings should be relevant for a better understanding of the underlying mechanisms leading to congenital deformities of tetrapod limbs.

尽管人类先天性肢体形成及其相对丰度的复杂性，但斑马鱼中的分子机制和特异性是骨盆鳍/后肢形成的特异性。我们建议使用各种遗传方法阐明斑马鱼骨盆鳍/后肢形成的分子调节。 To this end, (I) we will clone and examine a novel mutant with severe defects in the formation of pelvic fins and associated endoskeleton, (II) we will test the hypothesis that RA signaling is required for pelvic fin initiation and will examine an unexpected involvement in patterning the pelvic fin endoskeleton, and (III) we will develop transgenic tools that allow us to manipulate RA signaling, and ultimately any signaling途径，以组织特异性的方式和任何给定时间开发骨盆鳍。我们的工作一起应该为脊椎动物后肢发展的调节提供新的重要见解。我们的发现应该与更好地理解导致四肢先天性畸形的潜在机制有关。