I-Corps: Translation potential of an efficient method to generate live-attenuated and replication-defective DNA viruses for vaccine development

I-Corps：一种有效方法的转化潜力，可生成用于疫苗开发的减毒活病毒和复制缺陷型 DNA 病毒

• 批准号: 2420924
• 负责人: Dunbar Birnie
• 金额: $ 5万
• 依托单位: Rutgers University New Brunswick
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2024
• 资助国家: 美国
• 起止时间: 2024-04-01 至 2025-03-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2420924&HistoricalAwards=false
• 关键词: Corps; Translation; potential; efficient; method

The broader impact of this I-Corps project is the development of a technology that prepares pathogens (viruses, bacteria, etc.) for use in vaccines through a rapid, simplified, low-cost manufacturing process. The technology works on pathogens that contain DNA (“DNA pathogens”). This solution holds promise because the method can be used to generate vaccines against any DNA pathogen, making the technology a platform relevant in both human and animal health markets (e.g., companion animals and livestock). The resulting vaccines will not need to be kept cold during shipping and storage, so it will be easier to get vaccines to places around the world. This attribute, in addition to the rapidity with which pathogens can be readied for vaccine production, means that the technology has the potential to quickly address epidemics and combat vaccine-preventable diseases worldwide. Long term, the technology will be validated to generate bacterial vaccines, important for antibacterial resistance. This technology should be attractive to researchers and vaccine manufacturers.This I-Corps project utilizes experiential learning coupled with a first-hand investigation of the industry ecosystem to assess the translation potential of the technology. This solution is based on the development of a technology that uses the chemical centanamycin to damage the DNA of pathogens and thereby produce live-attenuated, replication-defective pathogens that can be used to make vaccines. Previous studies have incorporated data using human cytomegalovirus and other pathogens and have demonstrated that a treated pathogen can infect cells but not replicate due to damaged DNA. The platform promises two key technical innovations for the vaccine industry: 1) since the solution is DNA pathogen-agnostic, the technique shows promise across all DNA-containing pathogens and 2) vaccines made with centanamycin-treated pathogens may not need cold storage and shipping. This innovation alone would be significant for addressing vaccine-preventable diseases worldwide.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

I-Corps 项目的更广泛影响是开发一种技术，通过快速、简化、低成本的制造过程来制备用于疫苗的病原体（病毒、细菌等）。该技术适用于含有 DNA 的病原体。 （“DNA 病原体”）。该解决方案具有前景，因为该方法可用于生产针对任何 DNA 病原体的疫苗，从而使该技术成为人类和动物健康市场（例如伴侣动物和牲畜）的相关平台。期间不需要保持寒冷运输和储存，因此可以更容易地将疫苗运送到世界各地，除了可以快速准备好病原体用于疫苗生产之外，还意味着该技术有潜力快速应对流行病和对抗疫苗。 -从长远来看，该技术将被验证以生产细菌疫苗，这对细菌耐药性很重要。该技术应该对研究人员和疫苗制造商有吸引力。该 I-Corps 项目利用经验学习和第一手调查。产业生态系统评估该技术的转化潜力，该解决方案基于一项技术的开发，该技术使用化学物质森那霉素来破坏病原体的 DNA，从而产生可用于制造疫苗的减毒活病原体。研究整合了人类巨细胞病毒和其他病原体的数据，并证明病原体可以感染细胞，但由于 DNA 受损而无法复制。该平台有望为疫苗行业带来两项关键技术创新：1）因为处理的溶液是 DNA。由于该技术与病原体无关，因此在所有含有 DNA 的病原体中都显示出应用前景，并且 2) 用森那霉素处理的病原体制成的疫苗可能不需要冷藏和运输，这一创新本身对于解决全球疫苗可预防疾病的问题就具有重要意义。该奖项反映了 NSF 的成就。法定使命，并通过使用基金会的智力价值和更广泛的影响审查标准进行评估，被认为值得支持。