Collaborative Research: PurSUiT: A Backbone for the Peracarida

合作研究：PurSUiT：Peracarida 的支柱

• 批准号: 2321306
• 负责人: Sarah Gerken
• 金额: $ 45.33万
• 依托单位: University of Alaska Anchorage Campus
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2024
• 资助国家: 美国
• 起止时间: 2024-03-15 至 2028-02-29
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2321306&HistoricalAwards=false
• 关键词: Collaborative; Research; PurSUiT; Backbone; Peracarida

Peracarids are an extremely large and diverse group of crustacean animals that are found everywhere on Earth, from deserts to the deepest ocean trenches. The most familiar peracarids are the terrestrial isopods, commonly known as roly polies, that are frequently found under rocks or plant pots. There are deep sea and freshwater versions of roly polies. They come in the most amazing body shapes and sizes - peracarids do just about everything except fly. Despite being found everywhere, very little is known about the relationships within Peracarida, limiting understanding of how the group came to be so species-rich and variable. The central goal of this project is to resolve the relationships within Peracarida. At least 30 undergraduate students, three graduate students, and a postdoctoral researcher will be trained in molecular laboratory techniques, bioinformatics, peracarid taxonomy, identification and description of new species, data management, and career development. Three workshops will be held to train 31 graduate students and early career professionals in peracarid systematics, collection, identification, preservation, bioinformatics, and data management. Project outreach will include social media, outreach to schools in diverse districts (Anchorage, Los Angeles, and western Alabama), museum exhibits, and bilingual (Spanish/English) outreach in traditional broadcast media and a short documentary film.The central goal of this project is to provide a robust phylogenetic framework for Peracarida based on broad taxon sampling. Currently, no such framework exists, which impedes research into important questions raised by the extreme diversity displayed across peracarid morphology, ecology, systematics and life history. The project will use a target capture phylogenomic approach with probe design informed by three new genomes and 50 new transcriptomes, as well as publicly available transcriptomes and genomes. Biodiversity across the order will be assessed using an integrative taxonomic approach combining DNA barcoding of COI, 16S and 18S and traditional morphological approaches. Beyond the immediate scope of the current proposal, this work is expected to catalyze research across Peracarida by providing a phylogenetic framework and a comprehensive set of phylogenomic tools specialized for Peracarida.This project is jointly funded by the Division of Environmental Biology and the Established Program to Stimulate Competitive Research (EPSCoR).This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

甲壳类动物是一种极其庞大且多样化的甲壳类动物，从沙漠到最深的海沟，地球上到处都有它们的身影。最熟悉的甲虫是陆生等足类动物，通常被称为“不倒翁”，经常在岩石或花盆下发现。不倒翁聚合物有深海和淡水版本。它们的体型和大小都非常惊人——除了飞以外，甲壳类几乎无所不能。尽管随处可见，但人们对虾螈内部的关系知之甚少，这限制了人们对这个群体如何变得如此物种丰富和多变的了解。该项目的中心目标是解决 Peracarida 内部的关系。至少 30 名本科生、三名研究生和一名博士后研究员将接受分子实验室技术、生物信息学、囊虫分类学、新物种鉴定和描述、数据管理和职业发展方面的培训。将举办三场讲习班，培训 31 名研究生和早期职业专业人员，涉及甲虫系统学、收集、鉴定、保存、生物信息学和数据管理。项目外展将包括社交媒体、对不同地区（安克雷奇、洛杉矶和阿拉巴马州西部）的学校的外展、博物馆展览以及传统广播媒体和短片的双语（西班牙语/英语）外展。该项目的中心目标该项目的目的是基于广泛的分类单元抽样为 Peracarida 提供一个强大的系统发育框架。目前，尚不存在这样的框架，这阻碍了对囊虫形态学、生态学、系统学和生活史方面表现出的极端多样性所提出的重要问题的研究。该项目将使用目标捕获系统发育学方法，并根据三个新基因组和 50 个新转录组以及公开的转录组和基因组进行探针设计。将使用 COI、16S 和 18S DNA 条形码与传统形态学方法相结合的综合分类方法来评估整个目的生物多样性。除了当前提案的直接范围之外，这项工作预计将通过提供系统发育框架和一套专门针对 Peracarida 的系统发育工具来促进整个 Peracarida 的研究。该项目由环境生物学司和既定计划共同资助刺激竞争性研究 (EPSCoR)。该奖项反映了 NSF 的法定使命，并通过使用基金会的智力优点和更广泛的影响审查标准进行评估，被认为值得支持。