EFRI ELiS: Biosynthetic Additive Manufacturing of Living Building Materials

EFRI ELiS：活性建筑材料的生物合成增材制造

• 批准号: 2318057
• 负责人: Jinxing Li
• 金额: $ 200万
• 依托单位: Michigan State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-01 至 2027-08-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2318057&HistoricalAwards=false
• 关键词: EFRI; ELiS; Biosynthetic; Additive; Manufacturing

This award aims to integrate cutting-edge approaches in advanced manufacturing, synthetic biology, and materials science to transform microbe-engineered lignocellulosic biomass into a printable ink for biosynthetic additive manufacturing of 3D-printed living materials and structures for building applications. The integration of carefully designed microbial networks in printable lignocellulose inks will produce hierarchically structured organic-inorganic living composites with enhanced mechanical and thermal properties, increased carbon storage, self-healing capacity, and scalable and modular fabrication. The award’s vision is that the technical innovations will lead to positive social impacts through: fighting climate change with carbon-negative living materials; combating homelessness through smart and modular building materials production; and filling technical gaps in the nation's Biomanufacturing and Bioeconomy initiative. The research thrusts will be tightly coupled with comprehensive educational and outreach components, including new projects and contents in graduate courses, REU/K-12 activities such as Science Festival showcase, iGEM undergraduate team mentorships, living materials art exhibitions, and biomanufacturing outreach workshops to prepare future scientists and engineers from diverse backgrounds in the highly interdisciplinary research fields of biomanufacturing and living materials.The goal of the research is to adopt ecological concepts of biomineralization and repurpose the symbiotic principles of fungal-bacterial interactions to model and design living microbial networks within lignocellulosic-biomass-derived materials as ink for biosynthetic additive manufacturing and in situ modulation of the material's properties. The research objectives of this project include: 1) ink Development: Design, model, and produce biomass-derived, microbe-integrated, and printable biomaterials feedstock for in situ biomineral and biopolymer synthesis; 2) additive Biomanufacturing: 3D printing with living components to optimize the material's performance and customize the construction of living building materials and structures; 3) benchmarking and Optimization: characterize and optimize the performance of the 3D bioprinted materials and structures, including strength, thermal properties, self-healing capacities, fire resistance, and carbon storage. We will finally scale up ink production with local feedstock, and scale up the printability and biomanufacturing for Environmental Impact Analysis and Techno-Economic Assessment. The overarching focus will be on obtaining a better understanding of how the synergized interactions between microbe-consortia and inert components within the 3D-printed physical-biological system can be engineered to enhance the material's performance. The project will allow the exploitation of microbial interdependencies and fungal-bacteria interactions in novel ways to analyze their habitation, reproduction, metabolism, interaction, and biosafety in an engineered space across different time scales and physical dimensions. The project will also allow the PI to develop a new biomanufacturing process that seamlessly integrates 3D printing and the biosynthetic process for tailored materials design based on highly scalable and sustainable biomass materials as feedstock. Therefore, this project will enable novel strategies to magnify biomanufacturing capacity in hybrid living-manmade systems.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该奖项旨在将高级制造，合成生物学和材料科学的尖端方法整合到将微生物工程的木质纤维素生物量转换为可打印的墨水，以用于生物合成的材料，用于生物合成的3D打印材料和结构，用于建筑应用。在可打印的木质纤维素油墨中精心设计的微生物网络的整合将产生层次结构化的有机无机生活组合物，具有增强的机械和热性能，增加碳存储，自我修复能力以及可扩展和模块化制造。该奖项的愿景是，技术创新将通过碳阴性材料与气候变化产生积极的社会影响；通过智能和模块化的建筑材料生产来与无家可归者作斗争；并填补国家生物制造和生物经济计划中的技术空白。 The research thrusts will be tightly coupled with comprehensive educational and outreach components, including new projects and contents in graduate courses, REU/K-12 activities such as Science Festival showcase, iGEM undergraduate team mentality, living materials art exhibitions, and biomanufacturing outreach workshops to prepare future scientists and engineers from divers backgrounds in the highly interdisciplinary research fields of biomanufacturing and living materials.The goal of the research is为了采用生物矿化和重新利用真菌 - 细菌相互作用的共生原理的生态概念，以建模和设计木质纤维素 - 生物源性材料中的活微生物网络，作为生物合成添加剂制造的墨水，并在材料特性的原位调节中。该项目的研究目标包括：1）墨水开发：设计，模型和产生生物质衍生，微生物集成和可打印的生物材料原料，用于原位生物矿物和生物聚合物合成； 2）附加生物制造：3D打印带有生命组件，以优化材料的性能并自定义生存建筑材料和结构的构建； 3）基准测试和优化：表征和优化3D生物打印材料和结构的性能，包括强度，热性能，自我修复能力，耐火性和碳存储。我们最终将通过本地原料扩大墨水的生产，并扩大环境影响分析和技术经济评估的印刷性和生物制造。总体重点将在于更好地了解3D打印物理生物系统中微生物 - 康复论和惰性成分之间的协同相互作用，以增强材料的性能。该项目将允许以新颖的方式利用微生物相互依赖性和真菌 - 细菌相互作用，以分析其在不同时间尺度和物理维度的工程空间中的居住，繁殖，代谢，相互作用和生物安全。该项目还将允许PI开发一个新的生物制造过程，该过程将基于高度可扩展和可持续的生物量材料作为原料的量身定制材料设计，无缝整合3D打印和生物合成过程。因此，该项目将使新的策略能够扩大混合生活效果系统的生物制造能力。该奖项反映了NSF的法定任务，并使用基金会的知识分子优点和更广泛的影响审查标准，通过评估诚实地表示支持。