Postdoctoral Fellowship: OPP-PRF: Identifying Central and Peripheral Thermosensors in Eurythermal and Stenothermal Arctic Fishes

博士后奖学金：OPP-PRF：识别广温和窄温北极鱼类的中央和外周热传感器

• 批准号: 2317970
• 负责人: Julia York
• 金额: $ 35.86万
• 依托单位: University of Illinois at Urbana-Champaign
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2024
• 资助国家: 美国
• 起止时间: 2024-03-01 至 2026-02-28
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2317970&HistoricalAwards=false
• 关键词: Postdoctoral; Fellowship; OPP; PRF; Identifying

The Arctic Ocean is warming at a rate up to seven times the global average, endangering and shifting key subsistence and commercial fisheries. Fish populations are stressed by warming and simultaneously by ocean acidification and oxidation. This study aims to characterize the evolution of proteins that integrate signals of warming, changing pH, and oxidation in the sensory systems of three species of Arctic and sub-Arctic fish: the Arctic cod (Boreogadus saida), saffron cod (Eleginus gracilis), and Atlantic cod (Gadus morhua). Studying the evolution of these sensory mechanisms provides insight into the sensitivity and responses of fish to climate change. This work also has implications for understanding protein-induced pain, inflammation, and itch responses in humans. During the course of this research, two undergraduates from underrepresented groups in science will be mentored through a summer research program. A K-12 lesson plan will be developed that combines the insights from this project with Alaska Native science curricula and values and Next Generation Science Standards. A major goal of this work is to generate high-quality genomic and transcriptomic data across stenothermal and eurythermal Arctic fish species, but also across central thermosensory nervous tissue, peripheral thermosensory nervous tissue, non-thermosensory nervous tissue, and non-nervous somatic tissue. These data will be generated using a combination of 3rd (IsoSeq) and 2nd (RNASeq) generation sequencing technologies and be used to characterize transient receptor potential (TRP) channel sequences and expression patterns. TRP channel profiles will be compared using in silico tests of regulatory and structural evolution, including predicting protein structure using AlphaFold2. These profiles will be compared between species, tissues, and to stenothermal Antarctic fishes to investigate central and peripheral mechanisms of temperature sensation in fishes and convergent adaptation to cold. This work is the first investigation into the evolution of molecular thermosensor proteins in any Arctic species and the first to target mechanisms of thermosensation in the central nervous system of fishes. This project explores physiological mechanisms of climate adaptation in key sentinel species and expands the sequencing resources of the polar science community by generating the first long-read genome for Arctic cod, Boreogadus saida.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

北极海的变暖，最多是全球平均水平的七倍，危害关键的生存和商业渔业的七倍。通过海洋酸化和氧化同时加热，鱼类种群压力。这项研究旨在表征蛋白质在三种北极和亚北极鱼类的感觉系统中整合变暖，变化和氧化信号的蛋白质的演变：北极鳕鱼（Boreogadus Saida），saffron cod（eleeginus gracilis）和大西洋Cod（Gadus Morhua）。研究这些感觉机制的演变提供了对鱼类对气候变化的敏感性和反应的见解。这项工作也对了解人类蛋白质引起的疼痛，炎症和瘙痒反应具有影响。在这项研究过程中，将通过夏季研究计划对科学代表性不足的两名本科生进行指导。将制定一个K-12课程计划，将该项目的见解与阿拉斯加本地科学课程和价值观以及下一代科学标准相结合。这项工作的一个主要目的是在占热热和炎热北极鱼类中产生高质量的基因组和转录组数据，但也跨越了中央热敏神经组织，周围热增强神经组织，非心脏感增强神经组织以及非障碍体细胞组织。这些数据将使用第三（ISOSEQ）和第二（RNASEQ）生成测序技术的组合生成，并用于表征瞬态受体电位（TRP）通道序列和表达模式。在调节和结构进化的硅测试中，将比较TRP通道曲线，包括使用AlphaFold2预测蛋白质结构。这些剖面将在物种，组织和stephothermal南极鱼类之间进行比较，以研究鱼类温度感觉的中心和外周机制以及对寒冷的趋同适应性。这项工作是对任何北极物种中分子热传感器蛋白进化的首次研究，也是第一个针对鱼类中枢神经系统中热增效机理的靶向。该项目探索了关键哨兵物种气候适应的生理机制，并通过生成第一个用于北极鳕鱼的长阅读基因组，Boreogadus Saida来扩大了极地科学界的测序资源。该奖项反映了NSF的法定任务，并认为通过基金会的知识优点和广泛的criperia criperia criperia criperia criperia criperia criperia criperia ress criperia rection the奖。