Postdoctoral Fellowship: MPS-Ascend: Elucidation of Peptide Scrambling in Collision Induced Dissociation: Energetic and Mechanistic Analysis via Guided Ion Beam Mass Spectrometry

博士后奖学金：MPS-Ascend：阐明碰撞诱导解离中的肽加扰：通过引导离子束质谱进行能量和机理分析

• 批准号: 2316669
• 负责人: Evan Perez
• 金额: $ 30万
• 依托单位: Perez, Evan Hunt
• 依托单位国家: 美国
• 项目类别: Fellowship Award
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-08-01 至 2026-07-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2316669&HistoricalAwards=false
• 关键词: Postdoctoral; Fellowship; MPS; Ascend; Elucidation

Evan H. Perez is awarded an NSF Mathematical and Physical Sciences Ascending Postdoctoral Research Fellowship (NSF MPS-Ascend) to conduct a program of research and activities related to broaden participation by groups underrepresented in STEM. This fellowship to Dr. Perez supports the research project entitled “MPS-Ascend: Elucidation of Peptide Scrambling in Collision Induced Dissociation: Energetic and Mechanistic Analysis via Guided Ion Beam Mass Spectrometry” under the mentorship of a sponsoring scientist. The host institution for the fellowship is the University of Utah, and the sponsoring scientist is Dr. Peter B. Armentrout.This proposal seeks to deepen the understanding of peptide fragmentation and rearrangement mechanisms by using Guided Ion Beam Mass Spectrometry to observe energy-resolved collision events and model these energies using electronic structure calculations. The proposal will use polyglycine polymer ions to perform competitive threshold analysis and electronic structure calculations to elucidate the key chemical pathways for the observed fragmentation events. The proposed research will investigate the role of the peptide length from tetraglycine up to hexaglycine in the formation of macrocyclic structures which precedes the intramolecular fragmentation events known as “scrambling.” The work will implement traveling wave ion mobility SLIM (Structures for Lossless Ion Manipulation) experiments to allow for conformation specific fragmentation analysis as well as conformer analysis of the fragment ions. The proposed studies will enable comparison of fragmentation methods (hard single collision versus soft collisional heating) and correlation of conformation with mechanism. Successful implementation of this work may lead to improved understanding of peptide sequencing by mass spectrometry which could have significant impacts on determining protein sequences and advancing the proteomics field.The PI plans to mentor undergraduate and graduate students from underrepresented minority groups in research projects involving training in mass spectrometric techniques and learning quantum chemical calculations. The PI intends to participate with the STEM Action Center of the University of Utah in potential recruiting activities and mentoring of students from underrepresented groups.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

埃文·佩雷斯（Evan H. Perez）被授予NSF数学和物理科学，上升博士后研究奖学金（NSF MPS-ASCEND），以开展一项与STEM中代表性不足的团体扩大参与的研究和活动计划。佩雷斯博士的这一奖学金支持了题为“ MPS-ASCEND：碰撞引起的解离的肽的阐明：通过有导离子光束质谱法的能量和机械分析”的研究项目。奖学金的主机机构是犹他大学，赞助科学家是Peter B. ArmentRout博士。该提案旨在通过使用带导的离子光束质谱法来加深对肽碎片化和重排机制的理解，以观察能量溶解的碰撞事件，并使用电子结构来模拟这些能源。提案将使用聚糖聚合物离子进行竞争性阈值分析和电子结构计算，以阐明观察到的片段化事件的关键化学途径。拟议的研究将研究肽长度从四甘氨酸到己酰胺的作用，直至己酰胺在形成大分子内碎片事件之前的大环结构中的作用，称为“乱拼”。这项工作将实施无损离子操纵的行动波离子迁移率纤细（结构）实验，以允许片段离子的特定于会议特定的碎片分析以及构象异构体分析。拟议的研究将使碎裂方法（硬碰撞与软碰撞加热）以及构象与机制的相关性进行比较。这项工作的成功实施可能会导致通过质谱法对肽测序的了解得以提高，这可能会对确定蛋白质序列和推进蛋白质组学领域产生重大影响。PI计划在涉及质谱技术和学习量子化学量子化学量量的研究项目中，指导来自代表性不足的少数群体的本科生和研究生的PI计划。 PI打算与犹他大学的STEM行动中心一起参加潜在的招募活动和来自代表性不足的团体的学生的心理。该奖项反映了NSF的法定任务，并被认为是值得通过基金会的知识分子优点和更广泛的影响审查标准通过评估来进行评估的。