NSF PRFB FY 2023: Does long-term selection for growth on recalcitrant vs labile carbon impact Streptomyces nutrient use or inhibitory phenotypes?

NSF PRFB 2023 财年：顽固性碳与不稳定碳的长期选择是否会影响链霉菌的营养利用或抑制表型？

• 批准号: 2305753
• 负责人: Brett Lane
• 金额: $ 24万
• 依托单位: Lane, Brett
• 依托单位国家: 美国
• 项目类别: Fellowship Award
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-06-01 至 2026-05-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2305753&HistoricalAwards=false
• 关键词: NSF; PRFB; FY; 2023; Does

This action funds an NSF Postdoctoral Research Fellowship in Biology for FY 2023, Integrative Research Investigating the Rules of Life Governing Interactions Between Genomes, Environment, and Phenotypes. The fellowship supports research and training of the fellow that will contribute to the area of Rules of Life in innovative ways. This project will explore the impact of soil carbon complexity on microbial growth and interactions between microbes competing for limited carbon resources. Soil carbon can be broadly categorized as either readily available for microbial consumption (labile carbon) or difficult for microbial populations to metabolize (recalcitrant carbon). In order to utilize recalcitrant carbon, microbes must first break these complex compounds into their building blocks while also defending these building blocks from theft by neighboring microbes. To this end, microbes which specialize in the metabolism of recalcitrant carbon utilize antimicrobial compounds to prevent their neighbors from stealing these resources. These antimicrobial compounds are also believed to play a significant role in the suppression of soil-inhabiting, plant-pathogenic, organisms. As rising global temperatures are anticipated to increase the relative proportion of recalcitrant carbon within the soil, soil-inhabiting microbes are anticipated to alter their production of and resistance to antimicrobial compounds to further their ability to compete for these complex resources. Through this fellowship, the PI will further our understanding of how the adaptation of microbes to increasingly prominent recalcitrant carbon will influence interactions between soil-inhabiting microbes. The results of this research will be vital in promoting soil health and will ensure the protection of food crops from soil-inhabiting pathogens. Furthermore, this fellowship will further the training of the fellow, research personnel, graduate, and undergraduate students at the University of Minnesota as well as promote the participation and training of underrepresented groups in science.In this research, isolates of Streptomyces, a genus of prominent, soil-borne, antibiotic-producing bacteria, will undergo long-term selection for growth in petridishes containing a sole carbon source of varying recalcitrance. Isolates will be dotted on plates and grown until they exhibit sporulation. Spores will then be transferred to subsequent plates for a total of fifty generations of selection on either recalcitrant or labile carbon. It is anticipated that growth on recalcitrant carbon sources, in contrast to labile carbon, will impart selection for the utilization of a wider range of carbon sources and will increase the frequency and intensity of constitutive antibiotic production. Following fifty generations of growth, the fellow will characterize changes in isolate nutrient use and inhibitory phenotypes as well as genomic and transcriptional changes associated with carbon metabolism and secondary metabolite production. The results of this project will provide insight into the genomic and transcriptional modifications resulting from selection for growth on carbon sources of varying recalcitrancy. This project will further the fellow’s training in microbial ecology and genomic analysis as well as mentorship and scientific communication. The fellow will also recruit undergraduate researchers to train the next generation of scientists. In addition, the fellow will develop and conduct outreach events to broaden K-12 participation in science.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

这项行动资助了 2023 财年 NSF 生物学博士后研究奖学金，即研究基因组、环境和表型之间相互作用的生命规则的综合研究。该奖学金支持将为规则领域做出贡献的研究员的研究和培训。该项目将探讨土壤碳复杂性对微生物生长的影响以及争夺有限碳资源的微生物之间的相互作用。土壤碳可大致分为微生物可利用的碳。消耗（不稳定碳）或微生物群体难以代谢（顽固碳）为了利用顽固碳，微生物必须首先将这些复杂化合物分解成其组成部分，同时还要防止这些组成部分被邻近微生物窃取。专门从事顽固碳代谢的微生物利用抗菌化合物来防止其邻居窃取这些资源，这些抗菌化合物也被认为在抑制碳的产生方面发挥着重要作用。由于全球气温上升预计会增加土壤中顽固碳的相对比例，因此土壤微生物预计会改变其抗菌化合物的产生和耐药性，以增强其竞争能力。通过这项研究，项目负责人将进一步了解微生物对日益突出的顽固碳的适应将如何影响土壤中微生物之间的相互作用。这项研究的结果对于促进微生物的发展至关重要。此外，该奖学金还将进一步培训明尼苏达大学的研究员、研究人员、研究生和本科生，并促进以下人员的参与和培训。在这项研究中，链霉菌（一种重要的土传抗生素产生细菌属）的分离株将在含有不同顽抗性的唯一碳源的培养皿中进行长期选择。分离物将被点在平板上并生长，直到它们表现出孢子形成，然后将孢子转移到后续平板上，在顽抗性或不稳定碳源上进行总共五十代的选择，与不稳定碳源相反。碳，将为更广泛的碳源的利用提供选择，并将增加组成型抗生素生产的频率和强度，经过五十代的生长，该研究员将分离养分利用和抑制表型的变化描述为以及与碳代谢和次级代谢物产生相关的基因组和转录变化。该项目的结果将提供对不同顽抗性碳源的生长选择所导致的基因组和转录修饰的深入了解。该研究员还将招募本科生研究人员来培训下一代科学家。此外，该研究员还将开展和开展外展活动，以扩大 K-12 对科学的参与。通过使用基金会的智力价值和更广泛的影响审查标准进行评估，NSF 的法定使命被认为值得支持。