NSF PRFB FY 2023: Does long-term selection for growth on recalcitrant vs labile carbon impact Streptomyces nutrient use or inhibitory phenotypes?

NSF PRFB 2023 财年：顽固性碳与不稳定碳的长期选择是否会影响链霉菌的营养利用或抑制表型？

• 批准号: 2305753
• 负责人: Brett Lane
• 金额: $ 24万
• 依托单位: Lane, Brett
• 依托单位国家: 美国
• 项目类别: Fellowship Award
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-06-01 至 2026-05-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2305753&HistoricalAwards=false
• 关键词: NSF; PRFB; FY; 2023; Does

This action funds an NSF Postdoctoral Research Fellowship in Biology for FY 2023, Integrative Research Investigating the Rules of Life Governing Interactions Between Genomes, Environment, and Phenotypes. The fellowship supports research and training of the fellow that will contribute to the area of Rules of Life in innovative ways. This project will explore the impact of soil carbon complexity on microbial growth and interactions between microbes competing for limited carbon resources. Soil carbon can be broadly categorized as either readily available for microbial consumption (labile carbon) or difficult for microbial populations to metabolize (recalcitrant carbon). In order to utilize recalcitrant carbon, microbes must first break these complex compounds into their building blocks while also defending these building blocks from theft by neighboring microbes. To this end, microbes which specialize in the metabolism of recalcitrant carbon utilize antimicrobial compounds to prevent their neighbors from stealing these resources. These antimicrobial compounds are also believed to play a significant role in the suppression of soil-inhabiting, plant-pathogenic, organisms. As rising global temperatures are anticipated to increase the relative proportion of recalcitrant carbon within the soil, soil-inhabiting microbes are anticipated to alter their production of and resistance to antimicrobial compounds to further their ability to compete for these complex resources. Through this fellowship, the PI will further our understanding of how the adaptation of microbes to increasingly prominent recalcitrant carbon will influence interactions between soil-inhabiting microbes. The results of this research will be vital in promoting soil health and will ensure the protection of food crops from soil-inhabiting pathogens. Furthermore, this fellowship will further the training of the fellow, research personnel, graduate, and undergraduate students at the University of Minnesota as well as promote the participation and training of underrepresented groups in science.In this research, isolates of Streptomyces, a genus of prominent, soil-borne, antibiotic-producing bacteria, will undergo long-term selection for growth in petridishes containing a sole carbon source of varying recalcitrance. Isolates will be dotted on plates and grown until they exhibit sporulation. Spores will then be transferred to subsequent plates for a total of fifty generations of selection on either recalcitrant or labile carbon. It is anticipated that growth on recalcitrant carbon sources, in contrast to labile carbon, will impart selection for the utilization of a wider range of carbon sources and will increase the frequency and intensity of constitutive antibiotic production. Following fifty generations of growth, the fellow will characterize changes in isolate nutrient use and inhibitory phenotypes as well as genomic and transcriptional changes associated with carbon metabolism and secondary metabolite production. The results of this project will provide insight into the genomic and transcriptional modifications resulting from selection for growth on carbon sources of varying recalcitrancy. This project will further the fellow’s training in microbial ecology and genomic analysis as well as mentorship and scientific communication. The fellow will also recruit undergraduate researchers to train the next generation of scientists. In addition, the fellow will develop and conduct outreach events to broaden K-12 participation in science.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该行动为2023财年生物学的NSF博士后研究奖学金提供了资金，该研究综合研究调查了有关基因组，环境和表型之间相互作用的生活规则。奖学金支持对研究员的研究和培训，这些研究和培训将以创新的方式为生活规则做出贡献。该项目将探讨土壤碳复杂性对微生物生长的影响以及竞争有限碳资源的微生物之间的相互作用。土壤碳可以广泛地归类为可用于微生物消耗（不稳定碳），或者很难使微生物种群代谢（顽固碳）。为了利用顽固的碳，微生物必须首先将这些复杂化合物分解为其构建块，同时还通过相邻的微生物捍卫这些构成块。为此，专门研究顽固碳代谢的微生物利用抗菌化合物来防止其邻居窃取这些资源。这些抗菌化合物还被认为在抑制土壤居住，植物病原和生物体中起着重要作用。随着预计全球温度的升高将增加土壤中顽固碳的相对比例，预计土壤居住的微生物将改变其产量和对抗菌化合物的抵抗力，以进一步争夺这些复杂资源。通过这一奖学金，PI将进一步了解微生物如何适应突出的顽固碳的适应将影响土壤居住的微生物之间的相互作用。这项研究的结果对于促进土壤健康至关重要，并将确保粮食作物免受土壤居住的病原体的保护。此外，这项团契将进一步培训明尼苏达大学的研究人员，研究人员，毕业生和本科生，并促进科学中代表性不足的群体的参与和培训。在这项研究中，链霉菌的分离株，杰出的，土壤繁殖的人，抗生素生产的petrister selection属于成长的属属，是一位属于抗生素的成长，这些属于抗生素的成长，这些属于抗生素的成长，这些杂物的成长量是替代的。不同的顽固性。分离株将点缀在板上并生长，直到它们表现出孢子形成。然后，将孢子转移到随后的板上，在顽固或不稳定碳上进行总计五十代选择。可以预计，与不稳定的碳相反，顽固碳源上的生长将赋予使用多种碳源的选择，并会增加组成型抗生素产生的频率和强度。在五十代生长之后，该研究员将表征孤立营养用途和抑制性表型的变化，以及与碳代谢和继发代谢产生相关的基因组和转录变化。该项目的结果将提供有关选择因碳源不同的碳来源而导致的基因组和转录修饰的洞察力。该项目将进一步进一步研究微生物生态学和基因组分析以及指导和科学交流的培训。该研究员还将招募本科研究人员来培训下一代科学家。此外，该研究员将开发和进行外展活动，以扩大K-12的参与。该奖项反映了NSF的法定任务，并使用基金会的知识分子优点和更广泛的影响标准，被视为通过评估而被视为珍贵的支持。