SBIR Phase II: Bioelectrical Cell Enrichment, Sorting, and Recovery with On-Chip Sample Prep and Monitoring

SBIR 第二阶段：通过片上样品制备和监测进行生物电细胞富集、分选和回收

• 批准号: 2222933
• 负责人: Alexandra Hyler
• 金额: $ 95.26万
• 依托单位: CYTORECOVERY, INC.
• 依托单位国家: 美国
• 项目类别: Cooperative Agreement
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-02-15 至 2025-01-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2222933&HistoricalAwards=false
• 关键词: SBIR; Phase; II; Bioelectrical; Cell

The broader impact/commercial potential of this Small Business Innovation Research (SBIR) Phase II project is a label-free, biological sample preparation and cell isolation platform for recovery of rare cells from a variety of sample inputs. Specifically, by carrying out sample preparation, manipulation, and monitoring on a single chip platform for selective cell recovery, the sample handling steps will be greatly reduced, thereby ensuring the maintenance of cell viability, improving sample consistency, and sustaining native cell behaviors. This recovered sample is essential for the development of cell-based therapies in regenerative medicine and cancer management. Personalized medicine also requires precision cell recoveries.This Small Business Technology Transfer (STTR) Phase I project will develop an electrically functional microfluidic sample manipulation platform for phenotype-selective recovery of cells, based on their biophysical attributes. Specifically, microfluidic designs will be integrated to swap cells from complex biological inputs into an optimized buffer to enable cell manipulation. Also, instrumentation will be developed for on-chip monitoring of the sample during various stages of the phenotype-selective cell recovery process. Further, a variety of sample inputs will be validated on the designed devices to ensure performance, consistency, and reliability for translation into commercial product lines.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该小企业创新研究 (SBIR) 第二阶段项目的更广泛影响/商业潜力是一个无标记的生物样品制备和细胞分离平台，用于从各种样品输入中回收稀有细胞。具体来说，通过在单芯片平台上进行选择性细胞回收的样品制备、操作和监测，将大大减少样品处理步骤，从而确保细胞活力的维持、提高样品一致性并维持天然细胞行为。这种回收的样本对于再生医学和癌症管理中基于细胞的疗法的开发至关重要。个性化医疗还需要精确的细胞回收。这个小型企业技术转让 (STTR) 第一阶段项目将开发一种电功能微流体样品操作平台，用于根据细胞的生物物理属性对细胞进行表型选择性回收。具体来说，微流体设计将被集成，以将细胞从复杂的生物输入交换到优化的缓冲液中，以实现细胞操作。此外，还将开发用于在表型选择性细胞恢复过程的各个阶段对样品进行片上监测的仪器。此外，各种样本输入将在设计的设备上进行验证，以确保转化为商业产品线的性能、一致性和可靠性。该奖项反映了 NSF 的法定使命，并被认为值得通过使用基金会的智力优势和更广泛的影响审查标准。

项目成果

High-Frequency Dielectrophoresis Reveals That Distinct Bio-Electric Signatures of Colorectal Cancer Cells Depend on Ploidy and Nuclear Volume

高频介电泳揭示结直肠癌细胞的独特生物电特征取决于倍性和核体积

• DOI: 10.3390/mi14091723
• 发表时间: 2023-09-01
• 期刊: Micromachines
• 影响因子: 3.4
• 作者: Duncan JL;Bloomfield M;Swami N;Cimini D;Davalos RV
• 通讯作者: Davalos RV

Dielectrophoretic enrichment of live chemo-resistant circulating-like pancreatic cancer cells from media of drug-treated adherent cultures of solid tumors

从药物处理的实体瘤贴壁培养物的培养基中介电泳富集活的化疗耐药循环样胰腺癌细胞

• DOI: 10.1039/d3lc00804e
• 发表时间: 2024-01-30
• 期刊: Lab on a Chip
• 影响因子: 6.1
• 作者: Rane, Aditya;Jarmoshti, Javad;Siddique, Abdullah;Adair, Sara;Torres;Honrado, Carlos;Bauer, Todd W.;Swami, Nathan S.
• 通讯作者: Swami, Nathan S.