RUI: Developing Predictive Guidelines to Stabilize Gold Nanoparticles with Peptoids

RUI：制定用类肽稳定金纳米粒子的预测指南

• 批准号: 2305190
• 负责人: Amelia Fuller
• 金额: $ 33万
• 依托单位: Santa Clara University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-01 至 2026-08-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2305190&HistoricalAwards=false
• 关键词: RUI; Developing; Predictive; Guidelines; Stabilize

With the support of the Macromolecular, Supramolecular, and Nanochemistry Program in the Division of Chemistry, Professor Amelia A. Fuller of Santa Clara University will prepare new molecules and investigate their ability to stabilize gold nanoparticles in water. Gold nanoparticles have unique properties that make them important and versatile materials for medical diagnostics, for example. To function in water-rich environments for these applications, gold nanoparticles need to coated with specially tailored molecules. The molecular coatings are designed to prevent nanoparticles from forming insoluble, non-functional aggregates. Professor Fuller and undergraduate research assistants in her laboratory will investigate the use of a class of molecules called peptoids for their capacity to coat gold nanoparticles. Dozens of peptoids with different molecular properties will be synthesized and purified. The ability of each of these to prevent nanoparticle inactivation through aggregation will be measured. The research team will determine the specific molecular features of peptoids that confer optimal stability to the nanoparticles. Undergraduate students who participate in the research will cultivate their technical and problem-solving skills. Many of these students will be engaged through introductory-level coursework that incorporates research training. These experiences will equip a large cross-section of students with proficiencies they need for successful careers in science. Colloidal gold nanoparticles are versatile materials that have found wide-ranging applications that leverage their extraordinarily sensitive optical properties. To function in environmentally and physiologically relevant aqueous conditions, gold nanoparticles must be surface-modified with capping ligands. Ideal capping ligands form a well-packed layer on the nanoparticle surface that solubilizes the nanoparticle in aqueous media and shields the core from the salts and proteins that cause them to agglomerate. In this project, Professor Amelia A. Fuller and her group at Santa Clara University will prepare new capping ligands based on the peptoid (N-substituted glycine) scaffold, and investigate their ability to stabilize gold nanoparticles in water. Sequence-specific peptoids that incorporate a wide diversity of functional groups will be prepared. Their ability to stabilize gold nanoparticles of varied sizes will be evaluated. Ultimately, the Fuller laboratory aim to identify critical molecular features of peptoid capping ligands that confer optimal stability to gold nanoparticles. If successful, this understanding will enable the use of peptoid-capped nanoparticles in a wide variety of sensing and diagnostic applications.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

在化学系的大分子，超分子和纳米化学计划的支持下，圣克拉拉大学的Amelia A. Fuller教授将准备新分子，并研究其在水中稳定金纳米颗粒的能力。黄金纳米颗粒具有独特的特性，使其成为医学诊断的重要材料。为了在水丰富的环境中为这些应用起作用，金纳米颗粒需要涂有特殊量身定制的分子。分子涂层旨在防止纳米颗粒形成不溶性的非功能骨料。她的实验室中的富勒和本科研究助理教授将调查使用一类称为肽的分子，以涂上金纳米颗粒的能力。具有不同分子特性的数十个肽将被合成和纯化。将测量其中每一种防止纳米颗粒灭活的能力。研究小组将确定肽的特定分子特征，这些肽可以赋予纳米颗粒的最佳稳定性。参加研究的本科生将培养他们的技术和解决问题的技能。这些学生中的许多学生将通过结合研究培训的入门级课程来参与。这些经验将使大量的学生构成他们在科学领域成功职业所需的经验。胶体金纳米颗粒是多功能材料，它们发现了广泛的应用，可利用其非常敏感的光学特性。为了在环境和生理相关的水性条件下发挥作用，金纳米颗粒必须通过封盖配体进行表面修饰。理想的封盖配体在纳米颗粒表面形成了一个充满填充的层，该层在水性介质中溶解了纳米颗粒，并将核心免受盐和蛋白质的影响，从而导致它们凝聚。在这个项目中，Amelia A. Fuller教授及其在圣塔克拉拉大学的小组将根据肽（N-取代的甘氨酸）支架准备新的盖子配体，并研究其在水中稳定金纳米颗粒的能力。将制备结合各种官能团的序列特异性肽。他们将评估它们稳定各种大小的金纳米颗粒的能力。最终，富含实验室的目的是确定肽限制配体的关键分子特征，以赋予金纳米颗粒最佳稳定性。如果成功的话，这种理解将使在各种感应和诊断应用中使用肽限制的纳米颗粒。该奖项反映了NSF的法定任务，并被认为是值得通过基金会的知识分子优点和更广泛影响的评估来审查标准的评估值得支持的。