EAGER: PUF-based Probe Design for Secure Access to DNA Storage

EAGER：基于 PUF 的探针设计，用于安全访问 DNA 存储

• 批准号: 2300340
• 负责人: Leonidas Bleris
• 金额: $ 30万
• 依托单位: University of Texas at Dallas
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-02-15 至 2025-01-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2300340&HistoricalAwards=false
• 关键词: EAGER; PUF; based; Probe; Design

A functioning economy depends on the reliability of the goods and services being offered. Consumer goods from prescription or over-the-counter drugs to computers, automobiles, and appliances, all have unique individual or batch numbers. Raw materials used in every industry have similar identification labeling. As the bioeconomy develops, it will be necessary to uniquely identify all organisms in use. This will be important to establish strict biosafety and biosecurity protocols. This project will investigate a strategy to uniquely label individual strains of cells that will be inherited but not able to be replicated or read by a third party. This could help ensure the safety and security of a robust bioeconomy.The project will investigate the use of DNA as the core of an addressable, secure data storage system. Towards that end, the project team will pursue two aims. Aim 1 involves the use of CRISPR-technology to perform genome editing of cell lines for distributed information storage. They propose to investigate the properties of physically unclonable functions (PUFs) in a library of cell lines and characterize different genomic locations and assess the off-target effects of CRISPR editing. The second aim is to develop a prototype on-chip PUF probe. This probe will help determine whether a PUF key array can be implemented effectively.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

运作的经济取决于所提供的商品和服务的可靠性。从处方或非处方药到计算机，汽车和电器的消费品都有独特的个人或批量数。每个行业中使用的原材料都有相似的识别标签。随着生物经济的发展，有必要唯一地识别所有使用的生物。建立严格的生物安全和生物安全方案将很重要。该项目将调查一种策略，以唯一标记将继承但无法被第三方复制或阅读的单个单元素菌株。这可以有助于确保强大的生物经济学的安全和保障。该项目将调查DNA作为可寻址，安全数据存储系统的核心。为此，项目团队将追求两个目标。 AIM 1涉及使用CRISPR技术来对细胞系进行基因组编辑进行分布式信息存储。他们建议在细胞系中研究物理上无吻合功能（PUF）的特性，并表征不同的基因组位置，并评估CRISPR编辑的非目标效应。第二个目的是开发片上PUF探针的原型。该调查将有助于确定是否可以有效地实施PUF密钥阵列。该奖项反映了NSF的法定任务，并使用基金会的知识分子优点和更广泛的审查标准，认为值得通过评估值得支持。