EAGER: PUF-based Probe Design for Secure Access to DNA Storage

EAGER：基于 PUF 的探针设计，用于安全访问 DNA 存储

• 批准号: 2300340
• 负责人: Leonidas Bleris
• 金额: $ 30万
• 依托单位: University of Texas at Dallas
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-02-15 至 2025-01-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2300340&HistoricalAwards=false
• 关键词: EAGER; PUF; based; Probe; Design

A functioning economy depends on the reliability of the goods and services being offered. Consumer goods from prescription or over-the-counter drugs to computers, automobiles, and appliances, all have unique individual or batch numbers. Raw materials used in every industry have similar identification labeling. As the bioeconomy develops, it will be necessary to uniquely identify all organisms in use. This will be important to establish strict biosafety and biosecurity protocols. This project will investigate a strategy to uniquely label individual strains of cells that will be inherited but not able to be replicated or read by a third party. This could help ensure the safety and security of a robust bioeconomy.The project will investigate the use of DNA as the core of an addressable, secure data storage system. Towards that end, the project team will pursue two aims. Aim 1 involves the use of CRISPR-technology to perform genome editing of cell lines for distributed information storage. They propose to investigate the properties of physically unclonable functions (PUFs) in a library of cell lines and characterize different genomic locations and assess the off-target effects of CRISPR editing. The second aim is to develop a prototype on-chip PUF probe. This probe will help determine whether a PUF key array can be implemented effectively.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

经济的正常运转取决于所提供的商品和服务的可靠性。从处方药或非处方药到计算机、汽车和电器，消费品都有唯一的个体或批次号。每个行业使用的原材料都有类似的识别标签。随着生物经济的发展，有必要唯一地识别所有正在使用的生物体。这对于建立严格的生物安全和生物安保协议非常重要。该项目将研究一种策略，以独特地标记单个细胞株，这些细胞株将被遗传，但第三方无法复制或读取。这有助于确保强大的生物经济的安全性。该项目将研究使用 DNA 作为可寻址、安全数据存储系统的核心。为此，项目团队将追求两个目标。目标 1 涉及使用 CRISPR 技术对细胞系进行基因组编辑，以实现分布式信息存储。他们提议研究细胞系库中物理不可克隆功能（PUF）的特性，表征不同的基因组位置，并评估 CRISPR 编辑的脱靶效应。第二个目标是开发原型片上 PUF 探针。该调查将有助于确定 PUF 密钥阵列是否可以有效实施。该奖项反映了 NSF 的法定使命，并通过使用基金会的智力价值和更广泛的影响审查标准进行评估，被认为值得支持。