Functions of CURT1 proteins in thylakoid grana formation

CURT1蛋白在类囊体基粒形成中的功能

• 批准号: 254831856
• 负责人: Professor Dr. Dario Leister
• 金额: --
• 依托单位: Lehrstuhl für Molekularbiologie der Pflanzen
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2014
• 资助国家: 德国
• 起止时间: 2013-12-31 至 2017-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/254831856?language=en
• 关键词: Functions; CURT1; proteins; thylakoid; grana

Chloroplasts of land plants characteristically contain grana, cylindrical stacks of thylakoid membranes. The mechanisms leading to the formation of grana, and in particular the factors that generate the extreme curvature in grana margins are largely unknown. In previous work we have identified a small family of proteins, designated CURT1, which is required for grana formation. Because overexpresion of CURT1 proteins increases the number of membrane layers in grana, the CURT1 proteins are highly enriched in grana margins, and CURT1 proteins can induce tubulation of liposomes in vitro, we conclude that these proteins are directly involved in inducing/maintained the membrane curvature in margins. The five central questions which will be addressed in this project are: 1. By which mechanism do CURT1 proteins bend membranes? 2. What is the role of phosphorylation of thylakoid proteins (including CURT1) in grana stacking? 3. How has the grana stacking function of CURT1 protein evolutionary developed? 4. Is CURT1 sufficient in vivo to mediate grana stacking? Which other factors are involved? 5. What is the physiological relevance, in particular under natural conditions, of grana stacking? To address these questions we will apply methods from molecular genetics, molecular biology, biochemistry and electron microscopy (EM) and study several model species, in particular Arabidopsis thaliana, Synechocystis 6803 and Chlamydomonas reinhardtii.

土地植物的叶绿体特征在于绿叶膜的圆柱形堆栈。导致Grana形成的机制，尤其是在Grana边缘中产生极端曲率的因素在很大程度上未知。在先前的工作中，我们已经确定了一个指定的curt1的一小蛋白质，这是Grana形成所必需的。由于curt1蛋白的过度说明增加了Grana的膜层的数量，因此Curt1蛋白高度富集在Grana缘中，CORT1蛋白可以在体外诱导脂质体的脂质体，我们得出结论，这些蛋白质直接参与培养/维持膜层层中的膜层。该项目将要解决的五个核心问题是：1。curt1蛋白质弯曲膜的机制？ 2。类囊体蛋白（包括Curt1）在Grana堆叠中的磷酸化是什么？ 3。curt1蛋白进化的Grana堆叠功能如何发展？ 4。curt1在体内足以介导Grana堆叠？涉及哪些其他因素？ 5。谷物堆积的生理相关性，特别是在自然条件下是什么？为了解决这些问题，我们将采用分子遗传学，分子生物学，生物化学和电子显微镜（EM）的方法，并研究几种模型物种，特别是拟南芥，synechocystis 6803和renhardtii的衣原体。