Characterisation of the role of PAM71, PAM71L and AtCGL20 in the biogenesis and function of photosystem II and the NAD(P)H dehydrogenase

PAM71、PAM71L 和 AtCGL20 在光系统 II 和 NAD(P)H 脱氢酶的生物发生和功能中的作用的表征

• 批准号: 197471519
• 负责人: Professor Dr. Dario Leister
• 金额: --
• 依托单位: Lehrstuhl für Molekularbiologie der Pflanzen
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2011
• 资助国家: 德国
• 起止时间: 2010-12-31 至 2019-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/197471519?language=en
• 关键词: Characterisation; role; PAM71; PAM71L; AtCGL20

The assembly of the thylakoid multiprotein complexes photosystem II (PSII) and NAD(P)H dehydrogenase (NDH) proceeds through a number of distinct intermediate states and requires auxiliary proteins, the so-called assembly factors. We have previously identified and characterized the PSII assembly factor PAM68, and found in preparatory experiments that the sequence-related PAM68HL protein behaves like a NDH assembly factor. In addition, we found that lines with reduced expression of TerC, a protein essential for photosynthesis and plant development, as well as lines lacking the chloroplast protein PAM71, display phenotypes characteristic for PSII assembly mutants. We propose to characterize the proteins TerC and PAM71 and their association with PSII subunits and distinct assembly states, together with a detailed characterisation of the corresponding loss-of-function lines. To obtain a comprehensive picture of PSII assembly and the sequential action of PSII assembly factors, we will systematically analyse the interactions of TerC, PAM71 and PAM68 with PSII subunits, as well as with other PSII assembly factors, by the split-ubiquitin system, coim-munoprecipitation and bimolecular fluorescence complementation. Moreover, the role of PAM68HL in NDH assembly will be characterized by analysing loss-of-function lines and identifying interactors of PAM68HL by coimmunoprecipitation and bimolecular fluorescence complementation.

类囊体多蛋白复合物光系统 II (PSII) 和 NAD(P)H 脱氢酶 (NDH) 的组装经过许多不同的中间状态，需要辅助蛋白，即所谓的组装因子。我们之前已经鉴定并表征了 PSII 组装因子 PAM68，并在预备实验中发现序列相关的 PAM68HL 蛋白的行为类似于 NDH 组装因子。此外，我们发现 TerC（一种光合作用和植物发育必需的蛋白质）表达减少的品系，以及缺乏叶绿体蛋白 PAM71 的品系，显示出 PSII 组装突变体的特征表型。我们建议表征蛋白质 TerC 和 PAM71 及其与 PSII 亚基和不同组装状态的关联，以及相应功能丧失系的详细表征。为了全面了解 PSII 组装和 PSII 组装因子的顺序作用，我们将通过分裂泛素系统 coim 系统分析 TerC、PAM71 和 PAM68 与 PSII 亚基以及其他 PSII 组装因子的相互作用。 -免疫沉淀和双分子荧光互补。此外，PAM68HL 在 NDH 组装中的作用将通过分析功能丧失细胞系并通过免疫共沉淀和双分子荧光互补鉴定 PAM68HL 的相互作用物来表征。

项目成果

The Arabidopsis Protein CGL20 Is Required for Plastid 50S Ribosome Biogenesis1[OPEN]

拟南芥蛋白 CGL20 是质体 50S 核糖体生物发生所必需的 1[打开]

• DOI: 10.1104/pp.19.01502
• 发表时间: 2020-01-14
• 期刊: Plant Physiology
• 影响因子: 7.4
• 作者: Bennet Reiter;Evgenia Vamvaka;G. Marino;T. Kleine;P. Jahns;C. Bolle;D. Leister;Thilo Rühle
• 通讯作者: Thilo Rühle

Chlorophyll Fluorescence Video Imaging: A Versatile Tool for Identifying Factors Related to Photosynthesis

叶绿素荧光视频成像：识别光合作用相关因素的多功能工具

• DOI: 10.3389/fpls.2018.00055
• 发表时间: 2018
• 期刊: Frontiers in Plant Science
• 影响因子: 5.6
• 作者: Rühle T;Reiter B;Leister D
• 通讯作者: Leister D