Auxiliary factors for chlorophyll biosynthesis and their potential contribution to the assembly of chlorophyll into chlorophyll-binding proteins

叶绿素生物合成的辅助因子及其对叶绿素组装成叶绿素结合蛋白的潜在贡献

• 批准号: 254831670
• 负责人: Professor Dr. Bernhard Grimm
• 金额: --
• 依托单位: Arbeitsgruppe Pflanzenphysiologie
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2014
• 资助国家: 德国
• 起止时间: 2013-12-31 至 2021-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/254831670?language=en
• 关键词: Auxiliary; factors; chlorophyll; biosynthesis; their

Chlorophyll biosynthesis provides the essential pigments for photosynthesis in photoautotrophic organisms. While almost all enzymatic steps of have been elucidated, the spatio-temporal organization of the pathway as well as its functional coupling to the assembly of chlorophyll into photosynthetic proteins are still open questions. In continuation, the objective of this subproject is (i) the in-depth elucidation of the functional and structural roles of LIL3 (light-harvesting-like protein 3) and Pitt-homologous plastid-localized tetratricopeptide-repeat proteins (cpTPRs) for the organization of the late steps of chlorophyll biosynthesis and (ii) the examination of the functional roles of additional auxiliary factors which are required either for chlorophyll biosynthesis or the subsequent link to the assembly of chlorophyll-binding proteins, such as OHP (one-helix-protein), YCF54 (hypothetical chloroplast open reading frame 54) and CPL (Chaperone-like protein of POR1 (CPP1)-like protein). Their interaction partners are aimed to be newly identified or confirmed in wild-type and mutant plants by means of advanced biochemical methods, such as protein affinity chromatography and mass spectrometry, or fluorescence and electron microscopy (in part in cooperation with partners of the Research Unit). Thereby, these physiological and biochemical analyses contribute to the central objectives of the Research Unit to validate assembly factors required for the stepwise assembly of photosynthetically active protein complexes in thylakoid membranes.

叶绿素生物合成为光自营养生物的光合作用提供了必不可少的色素。尽管几乎所有的酶促步骤都已阐明，但该途径的时空组织及其功能耦合与叶绿素组装成光合蛋白的组合仍然是空的问题。在延续过程中，该副本的目的是（i）对LIL3（轻度收获样蛋白3）的功能和结构作用的深入阐明和PITT-同源性质体质体质肽重新肽重新肽蛋白（CPTPR）（CPTPR）的其他步骤，以II的作用，以及II的作用。叶绿素生物合成所需的辅助因子或随后与叶绿素结合蛋白组装的链接，例如OHP（OHP（单螺旋 - 蛋白质），YCF54（假设叶绿体开放式阅读框架54）和CPL（CPL）和CPL（CPL）（CPL）（CPL）（CPL）（por1（Cpp1）（Cpp1）（CPP1） - 蛋白质蛋白。他们的相互作用伙伴的目的是通过先进的生化方法在野生型和突变植物中新鉴定或确认，例如蛋白质亲和力色谱和质谱法，或荧光和电子显微镜（部分与研究单位的伙伴合作）。因此，这些生理和生化分析有助于研究单元的核心目标，以验证逐步组装诸如类囊体膜中光合作用活性蛋白复合物所需的组装因子。