CAREER: Tunable Graphene Microdevices for Multiplexed Detection of Biomolecules Beyond Diffusion Limit

职业：可调谐石墨烯微器件，用于超越扩散极限的生物分子的多重检测

基本信息

批准号：
2236997
负责人：
Seyedehaida Ebrahimi
金额：
$ 50万
依托单位：
Pennsylvania State Univ University Park
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
2023
资助国家：
美国
起止时间：
2023-04-01 至 2028-03-31
项目状态：
未结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=2236997&HistoricalAwards=false
关键词：
CAREER Tunable Graphene Microdevices Multiplexed

项目摘要

Existing and emerging electrochemical biosensors can achieve high sensitivity, specificity, and stability in detecting biochemical molecules. However, label-free and tunable sensors for detecting multiple biomolecules (multiplexed detection) with high sensitivity and short measurement time are still beyond maturity. The goal of this program is to lay the foundations for engineering novel graphene-based biosensors to address these needs with biogenic amine neurotransmitters as testbed. The research component of this CAREER program will significantly advance the field of electrochemical sensing by 1) enhancing our understanding of fundamental questions involving convergence of material-device-readout knobs in design of electrochemical sensors, revealing the effect of interface engineering and electrical gating on tuning sensor response, and elucidating the impact of multimodal sensing and data fusion on enhancing accuracy, specificity, and reliability, and 2) utilizing this knowledge in innovating a new class of reliable, multiplexed, and tunable devices. The research outcomes will provide a foundation for educational activities focused on training graduate and undergraduate students, including minorities and underrepresented groups. Multiple initiatives are integrated to increase public engagement in biosensing science and technology, including creating new laboratory modules for a biosensor-themed course, a summer workshop for teachers from districts underrepresented in STEM, and a summer camp for pre-college female students.This program aims at creating tunable and multiplexed electrochemical biosensors with high sensitivity, specificity and rapid response by developing new data-fused hybrid droplet-graphene microdevices. This CAREER program integrates both experimental and modeling investigations which include 1) elucidating the correlation between plasma-assisted functionalization of graphene and analyte-device interface engineering to enhance sensitivity and tune specificity of graphene microdevice array; 2) developing a learning-based multimodal electrochemical system for reliable classification of bioanalytes and multiplexing; 3) understanding the fundamental limits of sensitivity versus response time in a time-evolving system; 4) elucidating the fundamental mechanisms for electrical gating as an in-situ knob to tune the reaction kinetics at the graphene-analyte interface and hence the sensor response; and 5) demonstrating the application of this system with a well-studied drug screening neurosecretion cell model. This project is anticipated to have a long-term impact on biosensor engineering by elucidating how interface and device engineering influence detection limit and response time in time-evolving electrochemical systems and how convergence with multimodal readout can significantly enhance accuracy, specificity, and reliability of label-free diagnostics. In addition, the outcomes will advance utilization of the growing field of 2D material functionalization and devices in diagnostics, bioelectronics, and life science.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

现有和新兴的电化学生物传感器可以在检测生化分子时获得高灵敏度，特异性和稳定性。但是，用于检测多种生物分子（多路复用检测）的无标签和可调传感器具有高灵敏度和短测量时间的传感器仍然超出了成熟度。该程序的目的是为工程新型石墨烯的生物传感器奠定基础，以用生物胺神经递质作为测试床满足这些需求。该职业课程的研究组成部分将通过1）通过1）来大大推动电化学感测的领域，加强我们对材料 - 设备读取的旋钮融合在电化学传感器设计中的融合的理解，从而揭示接口工程的效果，并揭示了对传感器响应的效果，并揭示了对传感器响应的影响，并提高了多种模块化和数据融合的影响，并提高了多种模块化的影响，并提高了且准确性的范围，并提高了且准确性的范围。利用这些知识来创新新的可靠，多路复用和可调设备。研究成果将为专注于培训研究生和本科生（包括少数民族和代表性不足的群体）的教育活动提供基础。多项倡议旨在增加公众参与生物传感科学和技术，包括为以生物传感器为主题的课程创建新的实验室模块，为STEM中代表性不足的地区的教师提供夏季研讨会，以及为校长的夏令营，为校长的夏令营，以创建可调性和多重响应的高度敏感性和多重响应性的良好性及其多样性的良性，并通过良好的敏感性以及具有多种功能性的数据，并具有多种敏感性，并具有多种敏感性，并具有多种敏感性，并具有多种敏感性，并具有多种敏感性的生物良好性，并具有多种敏感性的生物效果，并具有良好的良好性能，并具有多样化的良性良好性。液滴 - 透明烯的微发频。该职业计划既集成了实验和建模研究，其中包括1）阐明石墨烯和分析物 - 设备接口工程的等离子体辅助功能之间的相关性，以增强石墨烯微型电视阵列的敏感性和调谐特异性； 2）开发一种基于学习的多模式电化学系统，用于可靠的生物分析和多重分类； 3）了解时间不断发展的系统中灵敏度与响应时间的基本限制； 4）阐明电子门控作为原位旋钮的基本机制，以调整石墨烯 - 分析物界面的反应动力学，从而调整传感器响应； 5）证明该系统使用精心研究的药物筛选神经分泌细胞模型。预计该项目通过阐明界面和设备工程如何影响检测限制和时间改进的电化学系统的响应时间以及与多模式读数的收敛如何显着提高准确性，特异性和无标签诊断的可靠性，从而对生物传感器工程产生长期影响。此外，结果将提高诊断，生物电子学和生命科学中2D物质功能化和设备的不断增长的领域的利用。该奖项反映了NSF的法定任务，并被认为是值得通过基金会的知识分子优点和更广泛的影响审查标准通过评估来进行评估的。