I-Corps: Commercialization of a real-time, palm-size, low-cost, and wireless SARS-COV-II detector

I-Corps：实时、手掌大小、低成本、无线 SARS-COV-II 探测器的商业化

• 批准号: 2230853
• 负责人: Zheng Tian
• 金额: $ 5万
• 依托单位: University of Arkansas
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-08-01 至 2024-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2230853&HistoricalAwards=false
• 关键词: Corps; Commercialization; real; time; palm

The broader impact/commercial potential of this I-Corps project is the development of a wireless nano-biosensor for detection of live coronavirus particles and potentially other pathogens. Currently, a positive result from an enzyme-linked immunosorbent assay (ELISA)- or polymerase chain reaction (PCR)-based COVID test does not determine if the coronavirus particles are live (infectious) or dead (or neutralized or noninfectious). The proposed technology is designed to detect interactions of live coronavirus particles with target cells, antibodies, and drugs in real-time. Those with a negative result from the proposed technology have confirmation that they are free of live, infectious coronavirus particles and would not need to quarantine. This technology may provide a tool for effectively combatting the COVID-19 virus and future pandemics/epidemics as well as other infections.This I-Corps project is based on the development of a nano-biosensing methodology for pathogen detection initially focused on SARS COV-II. The proposed technology integrates a bio-ceramic nanowire/graphene core/shell nanocomposite with a radio frequency identification (RFID) tag on a wireless communication chip surface. The hardware of the nano-biosensing system is composed of three parts: a radio frequency nano-sensor, a radio frequency reader, and a handheld device or smart phone. The proposed nano-biosensor is made by coating the biosafe nanocomposite on a disposable RFID tag to maximize the sensitivity. Further, nano-biosensors may be integrated to simultaneously detect samples in a multi-well plate, which is versatile and effective in the detection of virus particles, viral antigens, and antibodies, as well as in evaluating virus-neutralizing antibodies and vaccine efficacy.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该I-Corps项目的更广泛的影响/商业潜力是开发用于检测活冠状病毒颗粒和潜在其他病原体的无线纳米生物传感器。当前，酶连接的免疫吸附测定（ELISA）或基于聚合酶链反应（PCR）的共vid测试的阳性结果并不能确定冠状病毒颗粒是活的（感染性）或死亡（或中性化或中性化或非感染）。 该提出的技术旨在检测活冠状病毒颗粒与靶细胞，抗体和药物的相互作用。拟议技术的负面结果的人已经确认，他们没有生命，感染性冠状病毒颗粒，而不需要隔离。该技术可以提供一种工具，用于有效地对抗COVID-19病毒和未来的大流行病和其他感染。该I-Corps项目基于开发最初针对SARS COV-II的病原体检测的纳米生物传感方法。 提出的技术在无线通信芯片表面上集成了具有射频识别（RFID）标签的生物陶瓷纳米线/石墨烯核心/壳纳米复合材料。 纳米生物传感系统的硬件由三个部分组成：射频纳米传感器，射频读取器以及手持设备或智能手机。提出的纳米生物传感器是通过在一次性RFID标签上涂上生物安全纳米复合材料来制成的，以最大程度地提高灵敏度。此外，可以将纳米生物传感器整合到多孔板中的样品，该板在检测病毒颗粒，病毒抗原和抗体方面具有多功能且有效，并在评估病毒中性的抗体和疫苗的效果方面进行了评估，这反映了NSF的构图，并且在评估病毒中性抗体方面的启示。优点和更广泛的影响审查标准。