SBIR Phase I: Rapid, End-to-end Sample Preparation for Sequencing Applications

SBIR 第一阶段：用于测序应用的快速、端到端样品制备

• 批准号: 2222688
• 负责人: Ryan Montes
• 金额: $ 27.42万
• 依托单位: MICROPURE GENOMICS INC.
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-01-01 至 2024-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2222688&HistoricalAwards=false
• 关键词: SBIR; Phase; Rapid; End; end

The broader impact/commercial potential of this Small Business Innovation Research (SBIR) Phase I project consists of advancing methods for preparing deoxyribonucleic acid (DNA) for sequencing. Prior to sequencing a biological sample, DNA must be liberated from cells and separated from proteins and other unwanted debris, and then mixed with specialty buffers and chemical agents. This skilled task is currently carried out by trained scientists using largely manual manipulations of the samples and expensive equipment. The alternative method proposed in this project will speed-up diagnoses from genomic sequencing by significantly reducing preparation time while also making preparation more reliable through automation. Notably, the proposed approach is expected to prepare DNA without reducing its length; consequently, the process should be ideally suited for preparing samples for emerging long-read sequencing technologies. These improvements have the potential to decrease the burden and costs associated with DNA sequencing, hence expanding the benefits of DNA sequencing technology to wider segments of society.This Small Business Innovation Research (SBIR) Phase I project relies upon a process for trapping genomic material in a small flow cell through which an electric field and pressure-driven flow are simultaneously applied. The process is highly selective towards strands of DNA or (ribonucleic acid) RNA; proteins and other, unwanted debris that enters the flow cell passes through. Also, the process is relatively gentle, so the length of sample should not be shortened as a result. This project will advance the technology to the marketplace by: (1) Completing cartridge design details and fabrication, including evaluation of material options; (2) Building a custom research and development platform for interfacing with the cartridges; (3) Developing methods for DNA sample extraction and transfer; (4) Developing the library preparation protocols using mixing and heating while toggling the Electro-Hydrodynamic Trapping; and, (5) Integrating the entirety of the preparation process into a single cartridge and validating the process performance.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

这项小型企业创新研究（SBIR）I期项目的更广泛的影响/商业潜力由准备脱氧核糖核酸（DNA）进行测序的方法组成。在对生物样品进行测序之前，必须将DNA从细胞中释放，并与蛋白质和其他不需要的碎屑分离，然后与特殊的缓冲液和化学剂混合。这项熟练的任务目前是由训练有素的科学家使用样品和昂贵设备的手动操纵来执行的。该项目中提出的替代方法将通过显着减少制备时间来加快基因组测序的诊断，同时还通过自动化使制备更可靠。值得注意的是，所提出的方法有望在不减小其长度的情况下制备DNA。因此，该过程应理想地适合为新兴的长阅读测序技术准备样品。这些改进有可能减轻与DNA测序相关的负担和成本，从而将DNA测序技术的好处扩大到社会的更广泛的细分市场。这项小型企业创新研究（SBIR）I阶段项目依赖于将基因组材料捕获基因组材料的过程同时应用电场和压力驱动流动的小流动池。该过程对DNA或（核糖核酸）RNA的链高度选择性。进入流量细胞的蛋白质和其他不需要的碎片通过。同样，该过程相对较温和，因此不应缩短样品的长度。该项目将通过以下方式将技术推向市场，（1）完成墨盒设计细节和制造，包括评估材料选项； （2）建立一个定制的研发平台，用于与墨盒接口； （3）开发用于DNA样品提取和转移的方法； （4）使用混合和加热制定图书馆制备方案，同时切换电动力捕获； （5）将整个准备过程整合到单个墨盒中并验证过程性能。该奖项反映了NSF的法定任务，并使用基金会的知识分子优点和更广泛的影响审查标准，被认为值得通过评估来获得支持。