MRI: Acquisition of a FACSMelody Flow Cytometer and Cell Sorter

MRI：购买 FACSMelody 流式细胞仪和细胞分选仪

• 批准号: 2216005
• 负责人: Kinta Serve
• 金额: $ 26.3万
• 依托单位: Idaho State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-10-01 至 2025-09-30
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2216005&HistoricalAwards=false
• 关键词: MRI; Acquisition; FACSMelody; Flow; Cytometer

An award is made to Idaho State University to acquire a Fluorescence-Activated Cell Sorter (FACS) flow cytometer. This instrument will greatly enhance the research and educational opportunities across multiple departments and colleges at Idaho State University and at near-by undergraduate institution BYU-I. The instrument will be housed in a multi-user core facility and accessed by researchers and teachers in the Biological Sciences, Pharmaceutical Sciences, and Anthropology. Hands-on experimental design modules will be developed and utilized in various undergraduate and graduate courses, and within secondary education teacher-training courses. By incorporating this equipment into classes, students at all levels of education will gain hands-on experience in authentic research activities. This equipment will also be accessible to community partners and incorporated into university-wide outreach and STEM training activities to promote recruitment, retention, and professional development of a diverse workforce.This instrument will be utilized by a variety of researchers, from undergraduate students to professors. The ease of instrument set-up and diversity of applications across multiple disciplines will enhance current research capabilities and open opportunities for new and innovative projects. Researchers will use this machine to answer questions pertaining to 1) immune cell responses to environmental contaminants and physiologic stimuli, 2) cellular protein expression during organismal and cellular development, 3) plant genome/ploidy quantification, 4) hormone receptor expression in animal tissues, 5) gene expression in prokaryotes, 6) cell division and death, and 7) microparticle identification in archeologic samples (i.e., teeth, pottery). Many of these areas rely on the sorting functions of this machine to isolate live cells or small particles for further experimentation. Data generated using this instrument will be disseminated to the public via presentations and peer-reviewed papers, and data will impact a wide array of fields while providing cutting-edge training for students in environmental sciences, developmental biology, evolutionary biology, eukaryotic and prokaryotic cell biology, and anthropology.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

爱达荷州立大学获得荧光激活细胞分选仪 (FACS) 流式细胞仪的奖励。该工具将极大地增加爱达荷州立大学和附近本科院校杨百翰大学-I 多个系和学院的研究和教育机会。该仪器将安装在多用户核心设施中，供生物科学、药物科学和人类学领域的研究人员和教师使用。实践实验设计模块将在各种本科生和研究生课程以及中等教育教师培训课程中开发和使用。通过将这些设备融入课堂，各级教育的学生将在真实的研究活动中获得实践经验。该设备还将向社区合作伙伴开放，并纳入全校范围内的推广和 STEM 培训活动，以促进多元化劳动力的招聘、保留和专业发展。该仪器将被从本科生到教授的各种研究人员使用。仪器设置的简便性和跨学科应用的多样性将增强当前的研究能力，并为新的创新项目提供机会。研究人员将使用该机器回答以下问题：1) 免疫细胞对环境污染物和生理刺激的反应，2) 有机体和细胞发育过程中的细胞蛋白表达，3) 植物基因组/倍性定量，4) 动物组织中的激素受体表达， 5) 原核生物中的基因表达，6) 细胞分裂和死亡，以及 7) 考古样本（即牙齿、陶器）中的微粒鉴定。其中许多领域都依靠该机器的分选功能来分离活细胞或小颗粒以进行进一步的实验。使用该仪器生成的数据将通过演示文稿和同行评审论文向公众传播，数据将影响广泛的领域，同时为环境科学、发育生物学、进化生物学、真核和原核细胞的学生提供前沿培训该奖项反映了 NSF 的法定使命，并通过使用基金会的智力价值和更广泛的影响审查标准进行评估，被认为值得支持。