MRI: Acquisition of a High Pressure Stopped-Flow Instrument for Studying Biological Systems under Extreme Conditions

MRI：购买高压停流仪器，用于研究极端条件下的生物系统

• 批准号: 2213116
• 负责人: George Makhatadze
• 金额: $ 17.48万
• 依托单位: Rensselaer Polytechnic Institute
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-10-01 至 2025-09-30
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2213116&HistoricalAwards=false
• 关键词: MRI; Acquisition; Pressure; Stopped; Flow

An award is made to Rensselaer Polytechnic Institute to support the purchase of high-pressure stopped flow (HPSF) spectrophotometer/fluorimeter system. The instrument is the first of its kind in the US and will be housed in the RPI Analytical Biochemistry Core Facility. This will ensure that the unique cutting-edge capabilities of the instrument will be accessible to undergraduate students, graduate students, and postdoctoral fellows, not only at RPI but also to a broader community in the NY Capital Region and beyond. A number of laboratories will use the instrument to address questions of basic biochemistry, biology and evolutionary adaptations to extreme environments. The instrument will also be used in a number of hands-on workshops that will include undergraduate and graduate students. The participation in these workshops will be extended to the laboratories from NSF-supported Research Coordination Network on Extreme Biophysics (RCN-EB). In addition, the research that is enabled by this instrument will be incorporated in community outreach programs to encourage students into STEM fields and reaches out to talented underrepresented minorities and young women in the NY Capital Region. Hydrostatic pressure is an important environmental variable that plays an essential role in biological adaptation for many extremophilic organisms that inhabit the depth of the oceans and subsurface crusts. The HPSF instrument will support research activities of a group of investigators interested in understanding the molecular details of the effects of high hydrostatic pressure on the biological function and functional dynamics of biological macromolecules. The ability to experimentally monitor enzyme kinetics as a function of temperature and pressure using the HPSF instrument is critical for understanding of how hydrostatic pressure modulates the activity of the enzymes. This in turn will lead to a better understanding of adaptation of organisms to extreme pressures. High-hydrostatic pressure can also influence the energy landscape for protein folding. High-energy states of proteins are implicated in catalysis, binding, aggregation, allostery, and folding, thus connecting the enzymatic function with their dynamic properties. The results of these research efforts will be disseminated through active participation of students and faculty at scientific conferences, symposia and workshops, through peer-reviewed publications and public outreach presentations.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

伦斯勒理工学院获得奖励，支持购买高压停流 (HPSF) 分光光度计/荧光计系统。 该仪器是美国首台此类仪器，将安装在 RPI 分析生物化学核心设施中。这将确保不仅 RPI 的本科生、研究生和博士后能够使用该仪器独特的尖端功能，而且纽约首都地区及其他地区的更广泛社区也能使用该仪器的独特尖端功能。 许多实验室将使用该仪器来解决基本生物化学、生物学和极端环境的进化适应问题。 该仪器还将用于许多包括本科生和研究生在内的实践研讨会。 这些研讨会的参与范围将扩大到 NSF 支持的极端生物物理学研究协调网络 (RCN-EB) 的实验室。此外，该工具支持的研究将纳入社区外展计划，以鼓励学生进入 STEM 领域，并接触纽约首都地区有才华的、代表性不足的少数族裔和年轻女性。静水压力是一个重要的环境变量，对于栖息在海洋和地下地壳深处的许多极端微生物的生物适应起着至关重要的作用。 HPSF 仪器将支持一组研究人员的研究活动，这些研究人员有兴趣了解高静水压对生物大分子的生物功能和功能动力学影响的分子细节。 使用 HPSF 仪器对酶动力学作为温度和压力的函数进行实验监测的能力对于了解静水压力如何调节酶的活性至关重要。 这反过来将导致更好地了解生物体对极端压力的适应。 高静水压也会影响蛋白质折叠的能量格局。 蛋白质的高能态涉及催化、结合、聚集、变构和折叠，从而将酶功能与其动态特性联系起来。 这些研究工作的成果将通过学生和教师积极参与科学会议、研讨会和讲习班，通过同行评审的出版物和公共宣传演示来传播。该奖项反映了 NSF 的法定使命，并通过使用基金会的智力价值和更广泛的影响审查标准。