Collaborative Research: From cooperation to exploitation: context-dependent effects of nectar microbes on pollination mutualisms

合作研究：从合作到利用：花蜜微生物对授粉互惠关系的环境依赖性影响

• 批准号: 2211232
• 负责人: Robert Schaeffer
• 金额: $ 79.96万
• 依托单位: Utah State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-06-01 至 2025-05-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2211232&HistoricalAwards=false
• 关键词: Collaborative; Research; cooperation; exploitation; context

Cooperation is ubiquitous across ecological systems; yet how it continues to persist, even in the face of cheating, remains an enigma. This project will explore if and how microbes affect cooperation using plants and the microbes that reside in floral nectar as a model system. Across different systems, mutualistic species have been observed to steal floral resources without providing pollination services in return. This project uses a combination of field experiments across a community of wildflowers, floral visitors, and associated microbes in the Rocky Mountains. The main goals are to characterize (1) drivers of nectar microbial community structure; (2) how nectar microbes influence behaviors displayed by floral visitors; and (3) how microbes and the visitor behaviors affect plant reproduction. Broader impacts include student training, public outreach, and improved understanding of a major ecosystem service: pollination. The project will train undergraduate students, a graduate student, and a postdoctoral researcher. Public outreach is planned through a partnership with a non-profit museum, as well as through work with middle schools and summer science camps.Interactions among plants, pollinators, and nectar robbers have long served as an important model system for exploring ecological shifts between states of cooperation and conflict. Yet, despite their extensive study, the mechanisms that underlie shifts between these states, and constancy within, remain surprisingly unclear. Nectar microbes represent understudied but potentially significant interactors that may shape the foraging conditions under which floral visitors may decide to cooperate or cheat. This project will (1) employ the use of culture-dependent and culture-independent approaches to characterize the nectar microbiome of five montane wildflowers after legitimate vs. nectar robber floral visits; (2) assess how nectar microbes influence floral odor, nectar chemistry, and visitor behaviors; and (3) measure feedbacks of the microbes and floral visitor community on components of plant reproduction. By studying nectar microbes, their impact on floral phenotype, and whether they reinforce or facilitate the breakdown of pollination, we will gain a broader understanding of the factors that shape plant reproduction and pollination mutualisms.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

在生态系统中，合作无处不在。然而，即使面对作弊，它仍然如何继续存在仍然是一个谜。该项目将探讨微生物是否以及如何使用植物和含有花蜜作为模型系统的植物和微生物影响合作。在不同的系统中，已经观察到共同的物种可以窃取花卉资源，而无需提供授粉服务。该项目结合了在岩石山脉的野花，花卉游客和相关的微生物社区中进行的现场实验。主要目标是表征（1）花蜜微生物群落结构的驱动因素； （2）花蜜微生物如何影响花卉游客展示的行为； （3）微生物和访客行为如何影响植物的繁殖。更广泛的影响包括学生培训，公共宣传以及对主要生态系统服务的了解：授粉。该项目将培训本科生，研究生和博士后研究员。公众宣传是通过与非营利博物馆的合作伙伴关系以及与中学和夏季科学训练营的合作来计划的。植物，传粉媒介和花蜜强盗之间的互动长期以来一直是探索合作状态与冲突状态之间生态转变的重要模型系统。然而，尽管他们进行了广泛的研究，但这些状态与内部恒定的转变的机制仍然令人惊讶地不清楚。花蜜微生物代表经过研究的研究，但潜在的重要相互作用者可能会影响花卉游客决定合作或作弊的觅食条件。该项目将（1）利用与文化依赖性和与文化无关的方法来表征合法与花蜜强盗花卉访问之后五个山地野花的花蜜微生物组； （2）评估花蜜微生物如何影响花香，花蜜化学和游客行为； （3）测量微生物和花卉游客社区的反馈，以植物繁殖的组成部分。通过研究花蜜微生物，它们对花卉表型的影响以及它们是增强还是促进授粉的分解，我们将对塑造植物繁殖和授粉互助的因素有更广泛的了解。这奖反映了NSF的法定任务，并认为通过基金会的知识优点和广泛的crietia crietia crietia criteria criperia criperia criperia criperia rectiria criperia criperia criperia criperia均值得通过评估。

项目成果

The Floral Microbiome and Its Management in Agroecosystems: A Perspective

花卉微生物组及其在农业生态系统中的管理：一个视角

• DOI: 10.1021/acs.jafc.2c02037
• 发表时间: 2022
• 期刊: Journal of Agricultural and Food Chemistry
• 影响因子: 6.1
• 作者: Burgess, Emily C.;Schaeffer, Robert N.
• 通讯作者: Schaeffer, Robert N.