TRTech-PGR: Advancing Developmental Regulator (DR)-enabled and Genotype-independent Plant Transformation and Genome Editing (DR-CRISPR)

TRTech-PGR：推进发育调节器 (DR) 启用且与基因型无关的植物转化和基因组编辑 (DR-CRISPR)

• 批准号: 2206920
• 负责人: Feng Zhang
• 金额: $ 119.75万
• 依托单位: University of Minnesota-Twin Cities
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-08-01 至 2025-07-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2206920&HistoricalAwards=false
• 关键词: TRTech; PGR; Advancing; Developmental; Regulator

A major challenge for plant science is to produce sufficient food and plant-derived products for an ever-growing population despite a changing climate. Substantial public research investments have led to significant advances in decoding plant genetic and genomic information. Translation of this knowledge to create improved crops often requires genetic engineering or genome editing technology to develop or combine desired traits. At present, efficient genetic modifications are only achieved in a small number of crop species or varieties due to limitations of current plant transformation methods. The overall goal of this project is to develop an efficient and open-source genetic transformation and modification system for the agronomically important crop, soybean. The developed tools and resources can be easily extended to different soybean varieties and will be disseminated to the public research community for soybean crop improvement. Genome edited materials produced through this project will be used for public education in partnership with the K-12 Plant Genome Engineering Outreach and Market Science Programs organized at University of Minnesota.Recent progress in genome editing technology is poised to advance plant functional genomics and accelerate translational research for crop improvement. However, ubiquitous applications of genome editing are often hindered by the lack of efficient genetic transformation in many crop species. Recently, an emerging technology has provided the potential to significantly improve genotype-flexible plant regeneration and transformation by ectopic expression of developmental regulator (DR) genes in several crop species, such as maize, wheat and sorghum. However, additional work is needed to thoroughly investigate and extend the DR-mediated regeneration and transformation technology to other crops. This project is designed to develop a facile, genotype-independent and open-source plant transformation platform for efficient genetic engineering and genome editing in soybean. Research will be conducted to: 1) identify a suite of DR genes (or combinations of them) that enhance Agrobacterium-mediated transformation efficiency in soybean; 2) achieve a better understanding of the mechanisms underlying DR-mediated regeneration in soybean through gene regulatory network analysis; 3) develop efficient and open source DR-enabled genome editing toolkits that can be broadly applicable to different soybean varieties; and, 4) disseminate knowledge and train the next generation of scientists in cutting-edge plant transformation and genome editing technologies. The tools and resources developed in this proposal could be readily extended to other related crop species and will be made publicly available.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

植物科学的一个主要挑战是，尽管气候变化，但仍可为不断增长的人群生产足够的食品和植物衍生的产品。大量的公共研究投资导致了解码植物遗传和基因组信息的重大进展。这种知识的翻译以创造改进的农作物通常需要基因工程或基因组编辑技术来发展或结合所需的特征。目前，由于当前植物转化方法的局限性，仅在少数农作物或品种中实现有效的遗传修饰。该项目的总体目标是为农艺上重要的作物大豆开发有效的开源遗传转化和修饰系统。开发的工具和资源很容易扩展到不同的大豆品种，并将被传播到公共研究界以进行大豆作物的改善。通过该项目生产的基因组编辑的材料将与K-12植物基因组工程外展和市场科学计划合作进行公共教育。在明尼苏达大学组织的基因组编辑技术的结果有望促进植物功能基因组学和加速作物改进。但是，基因组编辑的普遍应用通常由于许多作物物种缺乏有效的遗传转化而阻碍。最近，一项新兴技术为在几种农作物，例如玉米，小麦和高粱等几种作物物种中的异位表达（例如发育调节剂（DR）基因的异位表达）显着改善了基因型富含基因型的植物再生和转化的潜力。但是，需要额外的工作来彻底研究并将DR介导的再生和转化技术扩展到其他农作物。该项目旨在开发一个易于基因型独立和开源植物转化平台，用于在大豆中有效的基因工程和基因组编辑。研究将进行：1）确定一套DR基因（或它们的组合），以提高大豆介导的农杆菌介导的转化效率； 2）通过基因调节网络分析更好地了解大豆中DR介导的再生的机制； 3）开发有效的开源DR基因组编辑工具包，可以广泛适用于不同的大豆品种；以及4）传播知识并培训下一代科学家在尖端的植物转化和基因组编辑技术方面。本提案中开发的工具和资源可以很容易地扩展到其他相关的农作物物种，并将公开使用。该奖项反映了NSF的法定任务，并被认为是值得通过基金会的知识分子优点和更广泛的影响评估标准来通过评估来支持的。

项目成果

An extensible vector toolkit and parts library for advanced engineering of plant genomes

• DOI: 10.1002/tpg2.20312
• 发表时间: 2023-03-09
• 期刊: PLANT GENOME
• 影响因子: 4.2
• 作者: Chamness, James C.;Kumar, Jitesh;Voytas, Daniel F.
• 通讯作者: Voytas, Daniel F.