Solution-State High Field DNP on Large Volume Samples: Sensitivity-Enchanced NMR for the Organic Chemist

大体积样品的溶液态高场 DNP：有机化学家的灵敏度增强 NMR

基本信息

批准号：
2203405
负责人：
Sungsool Wi
金额：
$ 45万
依托单位：
Florida State University
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2022
资助国家：
美国
起止时间：
2022-08-01 至 2025-07-31
项目状态：
未结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=2203405&HistoricalAwards=false
关键词：
Solution State Field DNP Large

项目摘要

With support from the Chemical Measurement and Imaging (CMI) Program in the Division of Chemistry, Dr. Sungsool Wi and his team at the National High Magnetic Field Laboratory (NHMFL) at Florida State University are devising new methods to advance the capabilities of nuclear magnetic resonance (NMR) spectroscopy – a leading means of characterizing the structure and dynamics of organic molecules. Complementary innovative paths are being pursued to substantially improve the NMR sensitivity and to provide correspondingly lower limits of detection for a wider range of target molecules. If successful, these methods would provide new opportunities for characterizing molecules in many different areas of scientific and industrial applications, including studies of pharmaceuticals, metabolites in human and animal studies, natural products, food and polymer additives, and small molecules in natural crude oil. Tools developed will be made available to local and remote users of NHMFL. This project provides valuable training opportunities to participating student and postdoctoral trainees, including summer internships offering hands-on experience for undergraduate students. The Wi team has identified two independent, complementary approaches to enhancing the NMR signals of arbitrary molecules. One relies on the use of low-viscosity supercritical solvents to enable electron-to-nucleus Overhauser-based dynamic nuclear polarization (ODNP). The other relies on hitherto unknown relaxation processes arising in biradicals to enable transfers from two J-coupled electrons to one nucleus over a wide variety of fields and correlation times. The Wi group will leverage these technological and conceptual breakthroughs to enable sensitivity-enhanced DNP spectroscopy at high fields on ~100 μL solution-state samples containing dilute organic molecules, with the aim of potentially creating a new paradigm in NMR.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

在化学划分的化学测量和成像（CMI）计划的支持下，佛罗里达州立大学国家高磁场实验室（NHMFL）的Sungsool Wi博士及其团队正在设计新的方法来提高核磁共振的能力（NMR）光谱范围（NMR）的能力，这是有机分类结构和动力学表征结构和动力学的领先手段。正在追求互补的创新路径，以实质上提高NMR敏感性，并为更广泛的目标分子提供相应的较低检测限制。如果成功的话，这些方法将为在许多不同领域的科学和工业应用领域中表征分子提供新的机会，包括对药物的研究，人类和动物研究中的代谢物，天然产物，食品和聚合物添加剂以及天然原油中的小分子。开发的工具将提供给NHMFL的本地和远程用户。该项目为参加学生和博士后学员提供了宝贵的培训机会，包括为本科生提供动手经验的暑期实习。 WI团队已经确定了两种独立的，完整的方法来增强任意分子的NMR信号。人们依靠使用低粘度超临界溶液来实现基于大肝癌的电子到核核能动态核极化（ODNP）。另一个依赖于迄今为止在Biradicals中产生的未知弛豫过程，可以使从两个J耦合电子转移到一个核心，以在各种场和相关时间内传输到一个核。 WI小组将利用这些技术和概念性突破来在〜100μL溶液状态样品上高领域的敏感性增强的DNP光谱范围，以便在NMR中创建一个新的范式，这些奖项在NMR中创建了新的范围，以众所周知的良好范围来评估NSF的众多范围，并有可能创建新的范式。标准。