CAREER: Electrochemiluminescence in Microfluidics for Mechanistic Studies of Redox Reactions and Single Particle Sensing

职业：微流体中的电化学发光用于氧化还原反应和单粒子传感的机理研究

• 批准号: 2145378
• 负责人: Frederique Deiss
• 金额: $ 65.1万
• 依托单位: Indiana University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-02-01 至 2027-01-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2145378&HistoricalAwards=false
• 关键词: CAREER; Electrochemiluminescence; Microfluidics; Mechanistic; Studies

With the support of the Chemical Measurement and Imaging (CMI) Program in the Division of Chemistry and partial funding from the Biosensing Program in the Division of Chemical, Bioengineering, Environmental, and Transport Systems (CBET), Frederique Deiss of Indiana University–Purdue University Indianapolis will study reduction-oxidation (redox) reactions and the detection of single particles with electrochemiluminescence (ECL) and microfluidics. To answer current challenges in analytical chemistry for the identification and measurements of a single entity, Dr. Deiss will integrate electrochemistry, microfluidics, spectroscopy, and materials sciences to produce methods for single-entity electrochemistry using ECL in droplets. ECL is the emission of light by a redox-active molecule after a cascade of electrochemical and chemical reactions. This research will provide new strategies to elucidate chemical mechanisms, with findings applied to making advances toward the goal of detecting single bacteria. If successful, this research will help society by generating a method for rapid and highly sensitive enumeration of bacteria, which is needed in fields such as clinical tests and industrial quality control, and to increase the understanding of chemical and biological systems. A sub-class of microfluidic devices are paper-based devices. Their versatility, low-cost, portability, and user-friendly features have enabled many studies, as well as point-of-care and in-field applications. The educational objective of this project is to transform the current knowledge and research in microfluidic paper-based analytical devices and produce low-cost and widely accessible platforms to teach practical analytical chemistry to students in a variety of settings via instructional videos. The video series of the lab modules will be hosted on free servers and educational websites to ensure its access to a broad audience. The paper-based analytical assay and video modules are expected to help motivate and spark interest in future researchers from all backgrounds, including underrepresented minorities in science-technology-engineering-mathematics (STEM).Concomitant to the development of the ECL single-entity electrochemistry (SEE) platform, the research objectives of this project, under the direction of Frederique Deiss of Indiana University–Purdue University Indianapolis, are to (i) study the different ECL pathways of common luminophore/co-reactant, (ii) explore other redox mechanisms such as the ones involved in bacterial metabolism used in resazurin-based redox indicators, (iii) detect bacteria at ultralow concentration, and (iv) generate paper-based platforms and instructional videos to teach hands-on analytical chemistry modules. Droplets generated on electrodes in a microfluidic device will yield an array of individual nodes to measure individual analytical events electrochemically and spectrally. The simultaneous collection of electrochemical and spectral data with space and time information should confer an edge to this SEE platform and help elucidate redox mechanisms. The mechanistic insights could help guide the development of other SEE droplet-based assays. This research will also ultimately expand the knowledge of electrochemistry at the interface of droplets and the new field of SEE. Additionally, its analytical performance is set to be demonstrated with a reverse ECL assay for the detection of bacterial cells down to the single cell level.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

借助化学测量和成像计划（CMI）计划，从化学，生物工程，环境和运输系统（CBET）的化学和部分资金划分中，印第安纳大学 - 普鲁尔特大学 - 普鲁尔特大学 - 印第安纳波利斯的弗雷德里克·迪斯（Frederique Deiss微流体学。为了应对单个实体的识别和测量分析化学方面的当前挑战，Deiss博士将使用滴水中的ECL整合电化学，微流体，光谱和材料科学，以生成单一实体电化学的方法。 ECL是一系列电化学和化学反应后通过氧化还原活性分子发射光的。这项研究将为阐明化学机制提供新的策略，并采用发现来实现检测单细菌的目标。如果成功，这项研究将通过生成一种快速且高度敏感的细菌列举的方法来帮助社会，这是在临床测试和工业质量控制等领域所需的，并增加对化学和生物系统的理解。微流体设备的子类是基于纸张的设备。它们的多功能性，低成本，可移植性和用户友好的功能使许多研究以及护理点和现场应用程序。该项目的教育目标是改变基于微流体的分析设备的当前知识和研究，并生成低成本且广泛可访问的平台，以通过教学视频在各种环境中向学生讲授实用的分析化学。实验室模块的视频系列将托管在免费服务器和教育网站上，以确保其访问广泛的受众。基于纸张的分析测定和视频模块有望有助于动机并引起对未来研究人员的兴趣，包括代表性不足的科学技术工程学 - 工程学 - 卡理学（STEM）。与ECL单一实体电化学平台（请参阅）计划的发展相关的是，在此项目的研究目标中，在Indian dep of Indistion the Indistion of Indistion the Indional dep dep of Indian dep the Indian dep the Indian dep the Indian dep of Indian dep of Indian dep of Indian dep od dep。 （i）研究常见的发光体/共同反应的不同ECL途径，（ii）探索其他氧化还原机制，例如在基于硫唑嗪的氧化还原指标中使用的细菌代谢涉及的机制，（iii）检测超出型浓度的细菌，以及（iv）（iv）（iv）（iv）生成基于纸张的平台和教学视频，以教授手持式化学分析。在微流体设备上产生的电子的液滴将产生一系列单个节点，以在电化学上和频谱上测量单个分析事件。具有空间和时间信息的电化学和光谱数据的简单集合应赋予该平台的优势，并有助于阐明氧化还原机制。机械见解可以帮助指导其他参见基于液滴的刺客的发展。这项研究还将最终扩大液滴界面和新领域的电化学知识。此外，其分析性能将通过反向ECL测定法证明，以检测细菌细胞至单细胞水平。该奖项反映了NSF的法定任务，并通过使用基金会的智力优点和更广泛的影响来诚实地认为，通过评估诚实地支持了审查标准。