MRI: Acquisition of an HPLC Coupled Mass Spectrometer for proteomics, metabolomics, and small molecule applications to expand and upgrade the SJSU PROTEIN Lab

MRI：购买用于蛋白质组学、代谢组学和小分子应用的 HPLC 耦合质谱仪，以扩展和升级 SJSU 蛋白质实验室

• 批准号: 2116424
• 负责人: Annalise Van Wyngarden
• 金额: $ 77.22万
• 依托单位: San Jose State University Foundation
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-10-01 至 2024-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2116424&HistoricalAwards=false
• 关键词: MRI; Acquisition; HPLC; Coupled; Mass

An award is made to San José State University (SJSU) to acquire a new HPLC-Mass Spectrometer instrument with improved metabolomics, proteomics and high-resolution small molecule capabilities for the SJSU core PROTEIN lab facility. San Jose State University is a federally recognized minority-serving institution. This instrument will benefit both current and future faculty across multiple disciplines by expanding the research capabilities at SJSU. The broader impacts of this proposal are to stimulate new research activities and strengthen collaboration among research faculty as well as continuing to train undergraduate and masters students and expose them to a state-of-the-art instrumentation in both classroom and research settings. Workshops will be developed to increase the number of faculty that will use the instrument in their research. A course in Chemical Biology will be revamped with a focus on the new instrument to provide valuable research and learning opportunities to students who are underrepresented in STEM fields. The intellectual merit of the supported research will (1) characterize how compounds found in fruits in vegetables play roles in cellular metabolism, (2) identify the molecular mechanisms in mammary gland development, (3) characterize bacteria that are able to metabolize and use rare earth elements for growth, (4) characterize the mechanism of action of combination therapies on inhibiting P. aeruginosa biofilm development, (5) understand how cellular pH plays a role in cellular processes including cell death, differentiation, and tumorigenesis, and (6) characterize small molecule development using a novel p450 biocatalysis platform.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

圣何塞州立大学 (SJSU) 获得奖励，为其核心蛋白质实验室设施购买一台新型 HPLC 质谱仪，该仪器具有改进的代谢组学、蛋白质组学和高分辨率小分子功能。圣何塞州立大学是联邦认可的少数派。该计划将通过扩展 SJSU 的研究能力，使当前和未来的多个学科的教师受益。该提案的更广泛影响是刺激新的研究活动并加强研究教师之间的合作。并将继续培训本科生和硕士生，让他们在课堂和研究环境中接触最先进的仪器，以增加在研究中使用该仪器的教师数量。化学生物学博士项目将进行改进，重点是新仪器，为 STEM 领域代表性不足的学生提供宝贵的研究和学习机会，所支持研究的智力价值将 (1) 描述水果和蔬菜中发现的化合物如何发挥作用。在细胞代谢中，（2）确定乳腺发育的分子机制，(3) 表征能够代谢和利用稀土元素生长的细菌，(4) 表征联合疗法抑制铜绿假单胞菌生物膜发育的作用机制，(5) 了解如何细胞 pH 在细胞过程中发挥作用，包括细胞死亡、分化和肿瘤发生，以及 (6) 使用新型 p450 生物催化平台表征小分子开发。该奖项反映了 NSF 的法定使命，并通过评估被认为值得支持利用基金会的智力优势和更广泛的影响审查标准。