Collaborative Research: Interactive physiological controls of trait expression, nutrient allocation, and the elemental stoichiometry of Synechococcus

合作研究：聚球藻性状表达、营养分配和元素化学计量的交互式生理控制

• 批准号: 2137339
• 负责人: Adam Martiny
• 金额: $ 62.39万
• 依托单位: University of California-Irvine
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-01 至 2025-08-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2137339&HistoricalAwards=false
• 关键词: Collaborative; Research; Interactive; physiological; controls

The phytoplankton elemental composition is a fundamental property of ocean science as variation in elemental ratios (C:N:P) have widespread implications for marine biodiversity, ecosystem functioning, and atmospheric CO2 levels. There are several competing hypotheses for mechanisms controlling phytoplankton elemental ratios including shifting biodiversity vs. cellular physiological responses to various environmental changes. Due to strong regional co-variance of all the possible drivers, each hypothesis predicts current ocean field observations equally well. To test each hypothesis, the project will employ continuous culture experiments of common phytoplankton strains combined with detailed biochemical analyses. All the data will be integrated into novel genome-based metabolic models. An outreach program aims at describing the importance of marine phytoplankton and ocean ecosystem functions to student groups visiting a local state park. Together, the proposed project will lead to an empirical and mechanistic understanding of how biological processes integrate to control plankton resource allocations in marine ecosystems. The C:N:P of phytoplankton is a fundamental property of ocean science and variation in these ratios have widespread implications for marine biodiversity, biogeochemical functioning and atmospheric CO2 levels. Competing hypotheses for mechanisms controlling marine phytoplankton C:N:P are shifting phytoplankton biodiversity vs. the physiological impacts of either nutrient limitation or temperature. Due to strong regional co-variance, each hypothesis predicts current ocean field observations equally well. The project will use strains representing ecotypes from major ocean biomes within the globally abundant Synechococcus ‘collective’. First, the elemental quotas and stoichiometric ratios will be quantified across a gradient of nutrient supply ratios (and limitations), temperature, and growth rates using chemostats. Second, the macromolecular cellular composition is quantified to directly link the observed elemental differences with changes in biochemical composition. Third, genome-wide protein analyses will be applied to identify how changes in growth conditions and elemental and biochemical composition are linked to the expression of specific cellular subsystems and associated functional traits. Fourth, experimental data will be integrated into a trait-based phytoplankton model to quantify the relative role of acclimation vs. adaptation. Together, the proposed project will lead to an empirical and mechanistic understanding of how processes at different levels of biological organization integrate to control C:N:P among marine phytoplankton.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

浮游植物元素组成是海科学的基本特性，因为元素比（c：n：p）的变化对海洋生物多样性，生态系统功能和大气二氧化碳水平具有广泛的影响。有几种相互竞争的假设，用于控制浮游植物元素比率的机制，包括将生物多样性与细胞生理反应转移到各种环境变化。由于所有可能的驱动因素的强大区域共变性，每个假设预测当前的海洋场观测值同样很好。为了检验每个假设，该项目将采用常见浮游植物菌株的连续培养实验以及详细的生化分析。所有数据将集成到基于基因组的新型代谢模型中。一项外展计划旨在描述海洋浮游植物和海洋生态系统功能对参观当地州立公园的学生团体的重要性。拟议的项目一起将导致对生物过程如何整合以控制海洋生态系统中的浮游生物资源分配的经验和机械理解。浮游植物的C：n：p是海科学的基本特性，这些比率的变化对海洋生物多样性，生物地球化学功能和大气二氧化碳水平具有宽敞的影响。控制海洋浮游植物C：N：P的机制的竞争假设是转移浮游植物生物多样性与营养限制或温度的生理影响。由于强大的区域共同变异，每个假设都可以很好地预测当前的海洋场观测值。该项目将使用代表来自全球丰富的Synechoccus“集体”中主要海洋生物群落的生态型的菌株。首先，将使用化学固醇的养分供应比（以及限制），温度和生长速率来量化元素引号和化学计量比。其次，将大分子细胞组成量化，以将观察到的元素差异与生化组成的变化直接联系起来。第三，全基因组蛋白分析将应用于确定生长条件以及元素和生化组成的变化如何与特定细胞子系统和相关功能性状的表达联系在一起。第四，实验数据将集成到基于特征的浮游植物模型中，以量化适应性与适应的相对作用。拟议的项目一起将导致对不同级别的生物组织整合过程中的过程的经验和机械理解，以控制C：N：P中的C：N：P。该奖项反映了NSF的法定任务，并被认为是通过基金会的知识分子和更广泛的影响审查标准来通过评估来通过评估来支持的。