Collaborative Research: Interactive physiological controls of trait expression, nutrient allocation, and the elemental stoichiometry of Synechococcus

合作研究：聚球藻性状表达、营养分配和元素化学计量的交互式生理控制

• 批准号: 2137339
• 负责人: Adam Martiny
• 金额: $ 62.39万
• 依托单位: University of California-Irvine
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-01 至 2025-08-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2137339&HistoricalAwards=false
• 关键词: Collaborative; Research; Interactive; physiological; controls

The phytoplankton elemental composition is a fundamental property of ocean science as variation in elemental ratios (C:N:P) have widespread implications for marine biodiversity, ecosystem functioning, and atmospheric CO2 levels. There are several competing hypotheses for mechanisms controlling phytoplankton elemental ratios including shifting biodiversity vs. cellular physiological responses to various environmental changes. Due to strong regional co-variance of all the possible drivers, each hypothesis predicts current ocean field observations equally well. To test each hypothesis, the project will employ continuous culture experiments of common phytoplankton strains combined with detailed biochemical analyses. All the data will be integrated into novel genome-based metabolic models. An outreach program aims at describing the importance of marine phytoplankton and ocean ecosystem functions to student groups visiting a local state park. Together, the proposed project will lead to an empirical and mechanistic understanding of how biological processes integrate to control plankton resource allocations in marine ecosystems. The C:N:P of phytoplankton is a fundamental property of ocean science and variation in these ratios have widespread implications for marine biodiversity, biogeochemical functioning and atmospheric CO2 levels. Competing hypotheses for mechanisms controlling marine phytoplankton C:N:P are shifting phytoplankton biodiversity vs. the physiological impacts of either nutrient limitation or temperature. Due to strong regional co-variance, each hypothesis predicts current ocean field observations equally well. The project will use strains representing ecotypes from major ocean biomes within the globally abundant Synechococcus ‘collective’. First, the elemental quotas and stoichiometric ratios will be quantified across a gradient of nutrient supply ratios (and limitations), temperature, and growth rates using chemostats. Second, the macromolecular cellular composition is quantified to directly link the observed elemental differences with changes in biochemical composition. Third, genome-wide protein analyses will be applied to identify how changes in growth conditions and elemental and biochemical composition are linked to the expression of specific cellular subsystems and associated functional traits. Fourth, experimental data will be integrated into a trait-based phytoplankton model to quantify the relative role of acclimation vs. adaptation. Together, the proposed project will lead to an empirical and mechanistic understanding of how processes at different levels of biological organization integrate to control C:N:P among marine phytoplankton.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

浮游植物元素组成是海洋科学的一个基本特性，因为元素比率 (C:N:P) 的变化对海洋生物多样性、生态系统功能和大气二氧化碳水平具有广泛的影响，对于控制浮游植物元素比率的机制存在多种相互竞争的假设，包括。生物多样性的变化与细胞对各种环境变化的生理反应由于所有可能的驱动因素具有很强的区域协方差，每个假设都能很好地预测当前的海洋实地观测结果，为了检验每个假设，该项目将采用常见的连续培养实验。浮游植物菌株与详细的生化分析相结合，将整合到基于基因组的新型代谢模型中，旨在向参观当地州立公园的学生团体描述海洋浮游植物和海洋生态系统功能的重要性。该项目将导致对生物过程如何整合以控制海洋生态系统中浮游生物资源分配的实证和机械理解。浮游植物的 C:N:P 是海洋科学的基本属性，并且这些比率的变化广泛存在。对海洋生物多样性、生物地球化学功能和大气二氧化碳水平的影响 控制海洋浮游植物 C:N:P 的机制的相互竞争的假设正在改变浮游植物的生物多样性与营养限制或温度的生理影响。该项目将使用代表全球丰富的聚球藻“集体”中主要海洋生物群落生态型的菌株，即元素配额和化学计量。使用恒化器对营养供应比率（和限制）、温度和生长速率的梯度进行量化；其次，对大分子细胞组成进行量化，以将观察到的元素差异与生化组成的变化直接联系起来。蛋白质分析将用于确定生长条件以及元素和生化成分的变化如何与特定细胞子系统和相关功能特征的表达联系起来。第四，实验数据将被整合到基于性状的浮游植物模型中。量化适应与适应的相对作用，拟议的项目将反映对不同生物组织水平的过程如何整合以控制海洋浮游植物中的 C:N:P 的经验和机械理解。该奖项是 NSF 的法定使命。通过使用基金会的智力价值和更广泛的影响审查标准进行评估，并被认为值得支持。