BRITE Fellow: Intelligent Nanoscale 3D Biomanufacturing for Human-on-a-Chip

BRITE 研究员：用于芯片人体的智能纳米级 3D 生物制造

• 批准号: 2135720
• 负责人: Shaochen Chen
• 金额: $ 100万
• 依托单位: University of California-San Diego
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-03-01 至 2027-02-28
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2135720&HistoricalAwards=false
• 关键词: BRITE; Fellow; Intelligent; Nanoscale; 3D; BRITE; Fellow; Intelligent; Nanoscale; 3D

This Boosting Research Ideas for Transformative and Equitable Advances in Engineering (BRITE) Fellow grant will provide a transformative nanoscale biomanufacturing platform powered by artificial intelligence. At the convergence of advanced additive manufacturing, stem cell biology, biomaterials, and biomechanics, biomanufacturing has the potential for creating three-dimensional biomimetic tissue constructs that can not only redefine the clinical capabilities of regenerative medicine but also transform the toolsets available for various applications such as disease modeling, pre-clinical drug screening, space exploratory and deep ocean studies with human-like tissues, and environmental health and safety studies using engineered human tissues. However, current biomanufacturing technologies have critical bottlenecks: a) they lack the resolution to resolve single cells and are too slow for fabricating a functional three-dimensional human tissues and organs, b) they are often conducted by trial and error, resulting in wasting expensive cells, biomaterials, and time, and c) the lack of systemic interactions with other tissues or organs at the human systemic level. This project aims to address fundamental research issues related to these bottlenecks. On the education side, a comprehensive equity, diversity, and inclusion plan will be developed. Graduate student researchers, undergraduate interns, and K-12 students from diverse backgrounds will have the opportunity to participate in this exciting research project and other outreach programs. As an eminent leader, the principal investigator is poised to make long lasting societal, educational, and commercial impacts on advanced manufacturing and nanoengineering. The research objectives of the project are to investigate ultrafast, near-field optics for nanoscale control of photo-polymerization in three-dimensional bioprinting, to investigate the machine learning methods for three-dimensional bioprinting, and to study the biomechanics and tissue functions of the bioprinted human-on-a-chip. To achieve these objectives, both theoretical and experimental investigations will be carried out to understand the nanoscale light control for bioprinting. Machine learning methods will be investigated for optimal control of the three-dimensional bioprinting process. The biomechanics issues and tissue functions in the human-on-a-chip will be studied. This will be the first attempt in the field to explore ultrafast near-field optics for bioprinting with nanoscale resolution. This research on machine learning methods is also quite novel, particularly for bioprinting so that traditional trial and error optimization will no longer be needed. Furthermore, the human-on-a-chip integrating major tissues such as heart, liver, lung, kidney in the microfluidic system will be an enabling platform for various applications. By integrating the emerging disruptive technologies in the multidisciplinary domains of biomanufacturing, artificial intelligence, and nanophotonics, this research supports exceptionally innovative, high risk, original, and unconventional research projects that have the potential to create new paradigms in advanced manufacturing.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

这一促进了工程学的变革性和公平进步的研究思想（BRITE）赠款将提供一个由人工智能提供支持的纳米级生物制造平台。在先进的添加剂制造，干细胞生物学，生物材料和生物力学的融合下，生物制造具有创建三维生物型组织结构的潜力设计的人体组织。 However, current biomanufacturing technologies have critical bottlenecks: a) they lack the resolution to resolve single cells and are too slow for fabricating a functional three-dimensional human tissues and organs, b) they are often conducted by trial and error, resulting in wasting expensive cells, biomaterials, and time, and c) the lack of systemic interactions with other tissues or organs at the human systemic level.该项目旨在解决与这些瓶颈有关的基本研究问题。在教育方面，将制定全面的公平，多样性和包容计划。来自不同背景的研究生研究人员，本科实习生和K-12学生将有机会参加这个令人兴奋的研究项目和其他外展计划。作为杰出的领导者，主要研究人员准备对高级制造和纳米工程产生持久的社会，教育和商业影响。该项目的研究目标是研究三维生物打印中光聚合的纳米级控制超快，近场光学，以研究用于三维生物涂纸的机器学习方法，并研究生物启动的人与A-A-A-Chip的生物力学和组织功能。为了实现这些目标，将进行理论和实验研究，以了解用于生物打印的纳米级光控制。将研究机器学习方法以最佳控制三维生物打印过程。将研究生物力学问题和组织功能。这将是该领域的第一次尝试，以探索超近场光学元件，以通过纳米级分辨率进行生物打印。这项关于机器学习方法的研究也非常新颖，特别是对于生物打印，因此不再需要传统的试验和错误优化。此外，微流体系统中的心脏，肝脏，肺，肾脏等主要组织的人类芯片将是各种应用的有利平台。通过将新兴的破坏性技术整合到生物制造，人工智能和纳米光子学的多学科领域中，这项研究支持了异常创新，高风险，原始和非常规的研究项目，这些项目有可能在高级制造业中创建新的范式，以反映NSF的知识范围，并具有NED STURID奖。影响审查标准。