Bioprinting of Complex Vascularized Tissues.

Functional vasculature is crucial for the maintenance of living tissues via the transport of oxygen, nutrients, and metabolic waste products. As a result, insufficient vascularization in thick engineered tissues will lead to cell death and necrosis due to mass transport and diffusional constraints. To circumvent these limitations, we describe the development of a microscale continuous optical bioprinting (μCOB) platform for 3D printing complex vascularized tissues with superior resolution and speed. By using the μCOB system, endothelial cells and other supportive cells can be printed directly into hydrogels with precisely controlled distribution and subsequent formation of lumen-like structures in vitro.

功能性脉管系统通过运输氧气、营养物质以及代谢废物产物，对活组织的维持至关重要。因此，在较厚的工程组织中血管化不足会因物质传输和扩散限制导致细胞死亡和坏死。为了规避这些局限性，我们描述了一种微尺度连续光学生物打印（μCOB）平台的开发，用于以超高分辨率和速度3D打印复杂的血管化组织。通过使用μCOB系统，内皮细胞和其他支持细胞能够以精确控制的分布直接打印到水凝胶中，并随后在体外形成管腔样结构。