Elucidating Fundamental Factors Driving Self-assembly with Guided Interactions in Multicomponent Enzyme Systems Using Model Nanostructured Platforms

使用模型纳米结构平台阐明多组分酶系统中通过引导相互作用驱动自组装的基本因素

• 批准号: 2108448
• 负责人: Cindy Berrie
• 金额: $ 50.85万
• 依托单位: University of Kansas Center for Research Inc
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-07-01 至 2025-06-30
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2108448&HistoricalAwards=false
• 关键词: Elucidating; Fundamental; Factors; Driving; Self; Elucidating; Fundamental; Factors; Driving; Self

With the support of the Macromolecular, Supramolecular and Nanochemistry program in the Division of Chemistry and the Established Program to Stimulate Competitive Research (EPSCoR), Professors Cindy L. Berrie and Candan Tamerler at the University of Kansas are investigating factors that govern the assembly and organization of biomolecules at interfaces. Affinity peptide tags will be used to selectively direct the self-assembly of biomolecules, including enzymes, onto material surfaces to create multicomponent bioactive materials organized at the nanoscale. The metal nanostructure platforms being developed are designed to enable an understanding of the role of material specificity, curvature, spacing, and size on the spatially organized self-assembly of biomolecules. The project will allow biohybrid materials to mimic the exquisite functionality nature has evolved for complex tasks, which will enable enhanced biosensing, biocatalysis and biofuel applications as an alternative energy source. In the course of conducting the project, graduate and undergraduate students will be trained in the growing convergence of nanoscience, biomolecules and biomaterials. In addition, the research team will carry out outreach and services to the community at the University of Kansas and local middle and elementary schools through participation in the Engineering EXPO and the Carnival of Chemistry events and the development of the “Science Night” program to engage students in science at an early stage. Public demonstrations on nanolithography and imaging will be conducted with the involvement of the students working on the project. The project focuses on exploring the fundamental factors responsible for peptide guided self-assembly of multi-enzyme systems using model nanostructured platforms to harness their coordinated activity. Nature exquisitely organizes cascades of enzymes to work in tandem; however, attempts to artificially assemble such complex systems are hampered by the complexity and lack of information about the factors governing functional assembly. Emerging applications from biocatalysis to biosensing, to energy harvesting and biofuels would likely benefit from assembly of coupled enzymes with cascade-like activity, and therefore elucidating the factors controlling the assembly of such complex systems would have wide ranging applications. Specifically, the assembly of peptide tags, peptide-labeled enzymes, and the co-assembly of coupled enzyme pairs will be investigated using optical and atomic force microscopy as well as bioactivity assays to determine the distribution, conformation, and orientation of assembled biomolecules and how these are affected by the metal nanostructure composition, spacing, size, and curvature. The scientific broader impacts of the work include the development of design principles for biohybrid materials for applications in biosensing and biocatalysis. There are also important elements of workforce development in the area of nanobiomaterials and of outreach the community.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

在化学系和启发竞争性研究的既定计划（EPSCOR）的大分子，超分子和纳米化学计划的支持下，堪萨斯大学的辛迪·贝里（Cindy L.亲和力胡椒标签将用于选择性地将包括酶在内的生物分子的自组装到材料表面上，以创建在纳米级组织的多组分生物活性材料。开发的金属纳米结构平台旨在了解材料特异性，曲率，间距和大小对生物分子的空间组织自组装的作用。该项目将允许生物杂交材料模仿复杂任务的等效功能性质，这将使增强的生物传感，生物催化和生物燃料应用成为替代能源。在进行该项目的过程中，毕业生和本科生将接受纳米科学，生物分子和生物材料的融合的培训。此外，研究团队将通过参与工程博览会和化学活动的狂欢节以及“科学”之夜”计划的发展，以在早期阶段与学生参与科学的发展，向堪萨斯大学以及当地中学和小学的社区进行外展和服务。将与纳米石像学和成像进行竞争，以竞争项目的参与。使用纳米结构平台的多种酶系统的自我组装来利用其协调性的活动。具有类似级联活性的耦合酶，因此阐明控制这种复杂系统组装的因素将具有广泛的应用。具体而言，将使用光学和原子力显微镜以及生物活性评估来研究肽标签的组装，肽标记的酶以及耦合酶对的共组装，并通过光学和原子力显微镜以及生物活性评估来确定由金属构造和分配的分配，并确定分布，会议和方向，并通过对这些构造的构造构成。这项工作的科学广泛影响包括为生物传感和生物催化中应用生物杂种材料的设计原理的发展。纳米生物材料和推广社区领域也有重要的劳动力发展元素。该奖项反映了NSF的法定使命，并使用基金会的知识分子优点和更广泛的影响审查标准，被认为是通过评估来获得的支持。